The Saccharomyces cerevisiae spindle pole body duplication gene MPS1 is part of a mitotic checkpoint

Eric Weiss, Mark Winey

Research output: Contribution to journalArticle

334 Citations (Scopus)

Abstract

M-phase checkpoints inhibit cell division when mitotic spindle function is perturbed. Here we show that the Saccharomyces cerevisiae MPS1 gene product, an essential protein kinase required for spindle pole body (SPB) duplication (Winey et al., 1991; Lauze et al., 1995), is also required for M-phase checkpoint function. In cdc31-2 and mps2-1 mutants, conditional failure of SPB duplication results in cell cycle arrest with high p34(CDC28) kinase activity that depends on the presence of the wild-type MAD1 checkpoint gene, consistent with checkpoint arrest of mitosis. In contrast, mps1 mutant cells fail to duplicate their SPBs and do not arrest division at 37°C, exhibiting a normal cycle of p34(CDC28) kinase activity despite the presence of a monopolar spindle. Double mutant cdc31-2, mps1-1 cells also fail to arrest mitosis at 37°C, despite having SPB structures similar to cdc31-2 single mutants as determined by EM analysis. Arrest of mitosis upon microtubule depolymerization by nocodazole is also conditionally absent in mps1 strains. This is observed in mps1 cells synchronized in S phase with hydroxyurea before exposure to nocodazole, indicating that failure of checkpoint function in mps1 cells is independent of SPB duplication failure. In contrast, hydroxyurea arrest and a number of other cdc mutant arrest phenotypes are unaffected by mps1 alleles. We propose that the essential MPS1 protein kinase functions both in SPB duplication and in a mitotic checkpoint monitoring spindle integrity.

Original languageEnglish (US)
Pages (from-to)111-123
Number of pages13
JournalJournal of Cell Biology
Volume132
Issue number1-2
DOIs
StatePublished - Jan 1 1996
Externally publishedYes

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Spindle Pole Bodies
M Phase Cell Cycle Checkpoints
Gene Duplication
Saccharomyces cerevisiae
Mitosis
Nocodazole
Hydroxyurea
Protein Kinases
Phosphotransferases
Spindle Apparatus
Essential Genes
Cell Cycle Checkpoints
S Phase
Microtubules
Cell Division
Alleles
Phenotype
Genes

ASJC Scopus subject areas

  • Cell Biology

Cite this

The Saccharomyces cerevisiae spindle pole body duplication gene MPS1 is part of a mitotic checkpoint. / Weiss, Eric; Winey, Mark.

In: Journal of Cell Biology, Vol. 132, No. 1-2, 01.01.1996, p. 111-123.

Research output: Contribution to journalArticle

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