The rub family of ubiquitin-like proteins: Crystal structure of arabidopsis RUB1 and expression of multiple rubs in arabidopsis

Chetana Rao-Naik, Wilfred DelaCruz, Jose M. Laplaza, Shirley Tan, Judy Callis, Andrew J Fisher

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Several proteins with significant identity to ubiquitin have been characterized recently. In contrast to ubiquitin's main role in targeting proteins for degradation, a described function of one family of ubiquitin- related proteins, the Rub family, is to serve as a stable post-translational modification of a complex involved in the G1-to-S cell cycle transition. Rub proteins have been found in animals, plants, and fungi and consist of 76 residues with 52-63% identity to ubiquitin. In this study three different RUB proteins within the plant Arabidopsis are identified; two differ by only 1 amino acid, while the third is only 77.6% identical to the other two. Genes encoding all three are expressed in multiple organs. In addition, we report the crystal structure of higher plant RUB1 at 1.7-Å resolution to help elucidate the functional differences between Rub and ubiquitin. RUB1 contains a single globular domain with a flexible COOH-terminal extension. The overall RUB1 structure is very similar to ubiquitin. The majority of the amino acid differences between RUB1 and ubiquitin map to the surface. These changes alter the electrostatic surface potential in two regions and likely confer specificity between ubiquitin and RUB1 and their ubiquitin-activating enzyme (E1) or E1-like activating enzymes.

Original languageEnglish (US)
Pages (from-to)34976-34982
Number of pages7
JournalJournal of Biological Chemistry
Volume273
Issue number52
DOIs
StatePublished - Dec 25 1998

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Ubiquitins
Ubiquitin
Arabidopsis
Crystal structure
Proteins
Ubiquitin-Activating Enzymes
Plant Structures
Amino Acids
Plant Proteins
Gene encoding
Post Translational Protein Processing
Fungi
Static Electricity
Proteolysis
Electrostatics
Cell Cycle
Animals
Cells
Degradation
Enzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

The rub family of ubiquitin-like proteins : Crystal structure of arabidopsis RUB1 and expression of multiple rubs in arabidopsis. / Rao-Naik, Chetana; DelaCruz, Wilfred; Laplaza, Jose M.; Tan, Shirley; Callis, Judy; Fisher, Andrew J.

In: Journal of Biological Chemistry, Vol. 273, No. 52, 25.12.1998, p. 34976-34982.

Research output: Contribution to journalArticle

Rao-Naik, Chetana ; DelaCruz, Wilfred ; Laplaza, Jose M. ; Tan, Shirley ; Callis, Judy ; Fisher, Andrew J. / The rub family of ubiquitin-like proteins : Crystal structure of arabidopsis RUB1 and expression of multiple rubs in arabidopsis. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 52. pp. 34976-34982.
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