The Ror1 receptor tyrosine kinase plays a critical role in regulating satellite cell proliferation during regeneration of injured muscle

Koki Kamizaki, Ryosuke Doi, Makoto Hayashi, Takeshi Saji, Motoi Kanagawa, Tatsushi Toda, So ichiro Fukada, Hsin-Yi Henry Ho, Michael Eldon Greenberg, Mitsuharu Endo, Yasuhiro Minami

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The Ror family receptor tyrosine kinases, Ror1 and Ror2, play important roles in regulating developmental morphogenesis and tissue- and organogenesis, but their roles in tissue regeneration in adult animals remain largely unknown. In this study, we examined the expression and function of Ror1 and Ror2 during skeletal muscle regeneration. Using an in vivo skeletal muscle injury model, we show that expression of Ror1 and Ror2 in skeletal muscles is induced transiently by the inflammatory cytokines, TNF-α and IL-1β, after injury and that inhibition of TNF-α and IL-1β by neutralizing antibodies suppresses expression of Ror1 and Ror2 in injured muscles. Importantly, expression of Ror1, but not Ror2, was induced primarily in Pax7-posi-tive satellite cells (SCs) after muscle injury, and administration of neutralizing antibodies decreased the proportion of Pax7-positive proliferative SCs after muscle injury. We also found that stimulation of a mouse myogenic cell line, C2C12 cells, with TNF-α or IL-1β induced expression of Ror1 via NF-κB activation and that suppressed expression of Ror1 inhibited their proliferative responses in SCs. Intriguingly, SC-specific depletion of Ror1 decreased the number of Pax7-positive SCs after muscle injury. Collectively, these findings indicate for the first time that Ror1 has a critical role in regulating SC proliferation during skeletal muscle regeneration. We conclude that Ror1 might be a suitable target in the development of diagnostic and therapeutic approaches to manage muscular disorders.

Original languageEnglish (US)
Pages (from-to)15939-15951
Number of pages13
JournalJournal of Biological Chemistry
Volume292
Issue number38
DOIs
StatePublished - Sep 22 2017

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Cell proliferation
Receptor Protein-Tyrosine Kinases
Muscle
Regeneration
Cell Proliferation
Satellites
Skeletal Muscle
Muscles
Interleukin-1
Muscle Cells
Wounds and Injuries
Neutralizing Antibodies
Organogenesis
Morphogenesis
Tissue regeneration
Cytokines
Cell Line
Animals
Chemical activation
Cells

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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The Ror1 receptor tyrosine kinase plays a critical role in regulating satellite cell proliferation during regeneration of injured muscle. / Kamizaki, Koki; Doi, Ryosuke; Hayashi, Makoto; Saji, Takeshi; Kanagawa, Motoi; Toda, Tatsushi; Fukada, So ichiro; Ho, Hsin-Yi Henry; Greenberg, Michael Eldon; Endo, Mitsuharu; Minami, Yasuhiro.

In: Journal of Biological Chemistry, Vol. 292, No. 38, 22.09.2017, p. 15939-15951.

Research output: Contribution to journalArticle

Kamizaki, K, Doi, R, Hayashi, M, Saji, T, Kanagawa, M, Toda, T, Fukada, SI, Ho, H-YH, Greenberg, ME, Endo, M & Minami, Y 2017, 'The Ror1 receptor tyrosine kinase plays a critical role in regulating satellite cell proliferation during regeneration of injured muscle', Journal of Biological Chemistry, vol. 292, no. 38, pp. 15939-15951. https://doi.org/10.1074/jbc.M117.785709
Kamizaki, Koki ; Doi, Ryosuke ; Hayashi, Makoto ; Saji, Takeshi ; Kanagawa, Motoi ; Toda, Tatsushi ; Fukada, So ichiro ; Ho, Hsin-Yi Henry ; Greenberg, Michael Eldon ; Endo, Mitsuharu ; Minami, Yasuhiro. / The Ror1 receptor tyrosine kinase plays a critical role in regulating satellite cell proliferation during regeneration of injured muscle. In: Journal of Biological Chemistry. 2017 ; Vol. 292, No. 38. pp. 15939-15951.
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