The role of TIR-NBS and TIR-X proteins in plant basal defense responses

Raja Sekhar Nandety, Jeffery L. Caplan, Keri Cavanaugh, Bertrand Perroud, Tadeusz Wroblewski, Richard W Michelmore, Blake C. Meyers

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Toll/interleukin receptor (TIR) domain-containing proteins encoded in the Arabidopsis (Arabidopsis thaliana) genome include the TIR-nucleotide binding site (TN) and TIR-unknown site/domain (TX) families. We investigated the function of these proteins. Transient overexpression of five TX and TN genes in tobacco (Nicotiana benthamiana) induced chlorosis. This induced chlorosis was dependent on ENHANCED DISEASE RESISTANCE1, a dependency conserved in both tobacco and Arabidopsis. Stable overexpression transgenic lines of TX and TN genes in Arabidopsis produced a variety of phenotypes associated with basal innate immune responses; these were correlated with elevated levels of salicylic acid. The TN protein AtTN10 interacted with the chloroplastic protein phosphoglycerate dehydrogenase in a yeast (Saccharomyces cerevisiae) two-hybrid screen; other TX and TN proteins interacted with nucleotide binding-leucine-rich repeat proteins and effector proteins, suggesting that TN proteins might act in guard complexes monitoring pathogen effectors.

Original languageEnglish (US)
Pages (from-to)1459-1472
Number of pages14
JournalPlant Physiology
Volume162
Issue number3
DOIs
StatePublished - Jul 2013
Externally publishedYes

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Interleukin Receptors
Plant Proteins
interleukins
plant proteins
Arabidopsis
receptors
Tobacco
Hypochromic Anemia
Proteins
proteins
Phosphoglycerate Dehydrogenase
chlorosis
Nucleotides
tobacco
Salicylic Acid
nucleotides
Innate Immunity
Genes
Saccharomyces cerevisiae
Nicotiana benthamiana

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

Cite this

Nandety, R. S., Caplan, J. L., Cavanaugh, K., Perroud, B., Wroblewski, T., Michelmore, R. W., & Meyers, B. C. (2013). The role of TIR-NBS and TIR-X proteins in plant basal defense responses. Plant Physiology, 162(3), 1459-1472. https://doi.org/10.1104/pp.113.219162

The role of TIR-NBS and TIR-X proteins in plant basal defense responses. / Nandety, Raja Sekhar; Caplan, Jeffery L.; Cavanaugh, Keri; Perroud, Bertrand; Wroblewski, Tadeusz; Michelmore, Richard W; Meyers, Blake C.

In: Plant Physiology, Vol. 162, No. 3, 07.2013, p. 1459-1472.

Research output: Contribution to journalArticle

Nandety, RS, Caplan, JL, Cavanaugh, K, Perroud, B, Wroblewski, T, Michelmore, RW & Meyers, BC 2013, 'The role of TIR-NBS and TIR-X proteins in plant basal defense responses', Plant Physiology, vol. 162, no. 3, pp. 1459-1472. https://doi.org/10.1104/pp.113.219162
Nandety RS, Caplan JL, Cavanaugh K, Perroud B, Wroblewski T, Michelmore RW et al. The role of TIR-NBS and TIR-X proteins in plant basal defense responses. Plant Physiology. 2013 Jul;162(3):1459-1472. https://doi.org/10.1104/pp.113.219162
Nandety, Raja Sekhar ; Caplan, Jeffery L. ; Cavanaugh, Keri ; Perroud, Bertrand ; Wroblewski, Tadeusz ; Michelmore, Richard W ; Meyers, Blake C. / The role of TIR-NBS and TIR-X proteins in plant basal defense responses. In: Plant Physiology. 2013 ; Vol. 162, No. 3. pp. 1459-1472.
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