The role of osmotic resistance on equine spermatozoal function.

Angela C. Pommer, Josep Rutllant, Stuart A Meyers

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Cryopreservation requires exposure of sperm to extreme variations in temperature and osmolality. The goal of this experiment was to determine the osmotic tolerance levels of equine sperm by analyzing motility, viability, mitochondrial membrane potential (MMP), and mean cell volume (MCV). Spermatozoa were incubated at 22 degrees C for 10 min in isosmolal TALP (300 mOsm/kg), or a range of anisosmolal TALP solutions (75-900 mOsm/kg), for initial analysis, and then returned to isosmolal conditions for 10 min for further analysis. Total sperm motility was lower (P < 0.05) in anisosmolal conditions compared to sperm motility in control medium. When cells were returned to isosmolal conditions, only sperm previously incubated in 450 mOsm/kg TALP were able to recover to control levels of motility. Sperm viability and MMP were lower (P < 0.05) when exposed to hypotonic solutions in comparison to control solutions. Sperm suspensions that were returned to isosmolal conditions from 75, 150, and 900 mOsm/kg had lower (P < 0.05) percentages of viable sperm than control suspensions (300 mOsm/kg). MMP was lower (P < 0.05) in cells previously incubated in 75 and 900 mOsm/kg when returned to isosmolal, as compared to control cells. MCV differed (P < 0.05) from control cell volume in all anisosmolal solutions. Cells in all treatments were able to recover initial volume when returned to isosmolal medium. Although most spermatozoa are able to recover initial volume after osmotic stress, irreversible damage to cell membranes may render some sperm incapable of fertilizing an oocyte following cryopreservation.

Original languageEnglish (US)
Pages (from-to)1373-1384
Number of pages12
JournalTheriogenology
Volume58
Issue number7
StatePublished - Oct 15 2002

Fingerprint

Horses
Spermatozoa
spermatozoa
horses
Sperm Motility
Mitochondrial Membrane Potential
membrane potential
Erythrocyte Indices
Cryopreservation
cells
sperm motility
cryopreservation
Suspensions
Hypotonic Solutions
viability
osmotolerance
Osmotic Pressure
Cell Size
osmolality
Osmolar Concentration

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

The role of osmotic resistance on equine spermatozoal function. / Pommer, Angela C.; Rutllant, Josep; Meyers, Stuart A.

In: Theriogenology, Vol. 58, No. 7, 15.10.2002, p. 1373-1384.

Research output: Contribution to journalArticle

Pommer, AC, Rutllant, J & Meyers, SA 2002, 'The role of osmotic resistance on equine spermatozoal function.', Theriogenology, vol. 58, no. 7, pp. 1373-1384.
Pommer, Angela C. ; Rutllant, Josep ; Meyers, Stuart A. / The role of osmotic resistance on equine spermatozoal function. In: Theriogenology. 2002 ; Vol. 58, No. 7. pp. 1373-1384.
@article{23ab4a25574144d1a95dd8fe35b10bf2,
title = "The role of osmotic resistance on equine spermatozoal function.",
abstract = "Cryopreservation requires exposure of sperm to extreme variations in temperature and osmolality. The goal of this experiment was to determine the osmotic tolerance levels of equine sperm by analyzing motility, viability, mitochondrial membrane potential (MMP), and mean cell volume (MCV). Spermatozoa were incubated at 22 degrees C for 10 min in isosmolal TALP (300 mOsm/kg), or a range of anisosmolal TALP solutions (75-900 mOsm/kg), for initial analysis, and then returned to isosmolal conditions for 10 min for further analysis. Total sperm motility was lower (P < 0.05) in anisosmolal conditions compared to sperm motility in control medium. When cells were returned to isosmolal conditions, only sperm previously incubated in 450 mOsm/kg TALP were able to recover to control levels of motility. Sperm viability and MMP were lower (P < 0.05) when exposed to hypotonic solutions in comparison to control solutions. Sperm suspensions that were returned to isosmolal conditions from 75, 150, and 900 mOsm/kg had lower (P < 0.05) percentages of viable sperm than control suspensions (300 mOsm/kg). MMP was lower (P < 0.05) in cells previously incubated in 75 and 900 mOsm/kg when returned to isosmolal, as compared to control cells. MCV differed (P < 0.05) from control cell volume in all anisosmolal solutions. Cells in all treatments were able to recover initial volume when returned to isosmolal medium. Although most spermatozoa are able to recover initial volume after osmotic stress, irreversible damage to cell membranes may render some sperm incapable of fertilizing an oocyte following cryopreservation.",
author = "Pommer, {Angela C.} and Josep Rutllant and Meyers, {Stuart A}",
year = "2002",
month = "10",
day = "15",
language = "English (US)",
volume = "58",
pages = "1373--1384",
journal = "Theriogenology",
issn = "0093-691X",
publisher = "Elsevier Inc.",
number = "7",

}

TY - JOUR

T1 - The role of osmotic resistance on equine spermatozoal function.

AU - Pommer, Angela C.

AU - Rutllant, Josep

AU - Meyers, Stuart A

PY - 2002/10/15

Y1 - 2002/10/15

N2 - Cryopreservation requires exposure of sperm to extreme variations in temperature and osmolality. The goal of this experiment was to determine the osmotic tolerance levels of equine sperm by analyzing motility, viability, mitochondrial membrane potential (MMP), and mean cell volume (MCV). Spermatozoa were incubated at 22 degrees C for 10 min in isosmolal TALP (300 mOsm/kg), or a range of anisosmolal TALP solutions (75-900 mOsm/kg), for initial analysis, and then returned to isosmolal conditions for 10 min for further analysis. Total sperm motility was lower (P < 0.05) in anisosmolal conditions compared to sperm motility in control medium. When cells were returned to isosmolal conditions, only sperm previously incubated in 450 mOsm/kg TALP were able to recover to control levels of motility. Sperm viability and MMP were lower (P < 0.05) when exposed to hypotonic solutions in comparison to control solutions. Sperm suspensions that were returned to isosmolal conditions from 75, 150, and 900 mOsm/kg had lower (P < 0.05) percentages of viable sperm than control suspensions (300 mOsm/kg). MMP was lower (P < 0.05) in cells previously incubated in 75 and 900 mOsm/kg when returned to isosmolal, as compared to control cells. MCV differed (P < 0.05) from control cell volume in all anisosmolal solutions. Cells in all treatments were able to recover initial volume when returned to isosmolal medium. Although most spermatozoa are able to recover initial volume after osmotic stress, irreversible damage to cell membranes may render some sperm incapable of fertilizing an oocyte following cryopreservation.

AB - Cryopreservation requires exposure of sperm to extreme variations in temperature and osmolality. The goal of this experiment was to determine the osmotic tolerance levels of equine sperm by analyzing motility, viability, mitochondrial membrane potential (MMP), and mean cell volume (MCV). Spermatozoa were incubated at 22 degrees C for 10 min in isosmolal TALP (300 mOsm/kg), or a range of anisosmolal TALP solutions (75-900 mOsm/kg), for initial analysis, and then returned to isosmolal conditions for 10 min for further analysis. Total sperm motility was lower (P < 0.05) in anisosmolal conditions compared to sperm motility in control medium. When cells were returned to isosmolal conditions, only sperm previously incubated in 450 mOsm/kg TALP were able to recover to control levels of motility. Sperm viability and MMP were lower (P < 0.05) when exposed to hypotonic solutions in comparison to control solutions. Sperm suspensions that were returned to isosmolal conditions from 75, 150, and 900 mOsm/kg had lower (P < 0.05) percentages of viable sperm than control suspensions (300 mOsm/kg). MMP was lower (P < 0.05) in cells previously incubated in 75 and 900 mOsm/kg when returned to isosmolal, as compared to control cells. MCV differed (P < 0.05) from control cell volume in all anisosmolal solutions. Cells in all treatments were able to recover initial volume when returned to isosmolal medium. Although most spermatozoa are able to recover initial volume after osmotic stress, irreversible damage to cell membranes may render some sperm incapable of fertilizing an oocyte following cryopreservation.

UR - http://www.scopus.com/inward/record.url?scp=0037107668&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037107668&partnerID=8YFLogxK

M3 - Article

C2 - 12387350

AN - SCOPUS:0037107668

VL - 58

SP - 1373

EP - 1384

JO - Theriogenology

JF - Theriogenology

SN - 0093-691X

IS - 7

ER -