The role of gamma interferon in infection of susceptible mice with murine coronavirus, MHV-JHM

Abigail L. Smith, Stephen W Barthold, M. S. de Souza, Kim Bottomly

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Infection of BALB/c mice with mouse hepatitis virus, strain JHM (MHV-JHM), at any of several intervals relative to ovalbumin (OVA) administration resulted in elevated OVA-specific IgG 2 a titers. Since gamma interferon (IFN) has been implicated as an up-regulator of IgG 2 a production, attempts were made to determine whether levels of this cytokine were modified in sera of infected mice. Serum IFN-γ was not detected, but treatment of MHV-JHM-infected mice with monoclonal anti-IFN-γ antibody resulted in high mortality with decreased survival times, enhanced virus titers in liver and spleen, and more severe virus-associated pathology, compared to mock-treated, infected mice. Immunotherapy with recombinant IFN-γ ameliorated disease as reflected by mortality rates and virus titers in target organs.

Original languageEnglish (US)
Pages (from-to)89-100
Number of pages12
JournalArchives of Virology
Volume121
Issue number1-4
DOIs
StatePublished - Mar 1991
Externally publishedYes

Fingerprint

Murine hepatitis virus
Coronavirus
Interferon-gamma
Interferons
Ovalbumin
Infection
Viral Load
Immunoglobulin G
Mortality
Serum
Immunotherapy
Anti-Idiotypic Antibodies
Spleen
Monoclonal Antibodies
Pathology
Cytokines
Viruses
Liver

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Genetics

Cite this

The role of gamma interferon in infection of susceptible mice with murine coronavirus, MHV-JHM. / Smith, Abigail L.; Barthold, Stephen W; de Souza, M. S.; Bottomly, Kim.

In: Archives of Virology, Vol. 121, No. 1-4, 03.1991, p. 89-100.

Research output: Contribution to journalArticle

Smith, Abigail L. ; Barthold, Stephen W ; de Souza, M. S. ; Bottomly, Kim. / The role of gamma interferon in infection of susceptible mice with murine coronavirus, MHV-JHM. In: Archives of Virology. 1991 ; Vol. 121, No. 1-4. pp. 89-100.
@article{f51e073b7eec4ba89268fdb28120868c,
title = "The role of gamma interferon in infection of susceptible mice with murine coronavirus, MHV-JHM",
abstract = "Infection of BALB/c mice with mouse hepatitis virus, strain JHM (MHV-JHM), at any of several intervals relative to ovalbumin (OVA) administration resulted in elevated OVA-specific IgG 2 a titers. Since gamma interferon (IFN) has been implicated as an up-regulator of IgG 2 a production, attempts were made to determine whether levels of this cytokine were modified in sera of infected mice. Serum IFN-γ was not detected, but treatment of MHV-JHM-infected mice with monoclonal anti-IFN-γ antibody resulted in high mortality with decreased survival times, enhanced virus titers in liver and spleen, and more severe virus-associated pathology, compared to mock-treated, infected mice. Immunotherapy with recombinant IFN-γ ameliorated disease as reflected by mortality rates and virus titers in target organs.",
author = "Smith, {Abigail L.} and Barthold, {Stephen W} and {de Souza}, {M. S.} and Kim Bottomly",
year = "1991",
month = "3",
doi = "10.1007/BF01316746",
language = "English (US)",
volume = "121",
pages = "89--100",
journal = "Archives of Virology",
issn = "0304-8608",
publisher = "Springer Wien",
number = "1-4",

}

TY - JOUR

T1 - The role of gamma interferon in infection of susceptible mice with murine coronavirus, MHV-JHM

AU - Smith, Abigail L.

AU - Barthold, Stephen W

AU - de Souza, M. S.

AU - Bottomly, Kim

PY - 1991/3

Y1 - 1991/3

N2 - Infection of BALB/c mice with mouse hepatitis virus, strain JHM (MHV-JHM), at any of several intervals relative to ovalbumin (OVA) administration resulted in elevated OVA-specific IgG 2 a titers. Since gamma interferon (IFN) has been implicated as an up-regulator of IgG 2 a production, attempts were made to determine whether levels of this cytokine were modified in sera of infected mice. Serum IFN-γ was not detected, but treatment of MHV-JHM-infected mice with monoclonal anti-IFN-γ antibody resulted in high mortality with decreased survival times, enhanced virus titers in liver and spleen, and more severe virus-associated pathology, compared to mock-treated, infected mice. Immunotherapy with recombinant IFN-γ ameliorated disease as reflected by mortality rates and virus titers in target organs.

AB - Infection of BALB/c mice with mouse hepatitis virus, strain JHM (MHV-JHM), at any of several intervals relative to ovalbumin (OVA) administration resulted in elevated OVA-specific IgG 2 a titers. Since gamma interferon (IFN) has been implicated as an up-regulator of IgG 2 a production, attempts were made to determine whether levels of this cytokine were modified in sera of infected mice. Serum IFN-γ was not detected, but treatment of MHV-JHM-infected mice with monoclonal anti-IFN-γ antibody resulted in high mortality with decreased survival times, enhanced virus titers in liver and spleen, and more severe virus-associated pathology, compared to mock-treated, infected mice. Immunotherapy with recombinant IFN-γ ameliorated disease as reflected by mortality rates and virus titers in target organs.

UR - http://www.scopus.com/inward/record.url?scp=0026380426&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026380426&partnerID=8YFLogxK

U2 - 10.1007/BF01316746

DO - 10.1007/BF01316746

M3 - Article

C2 - 1662041

AN - SCOPUS:0026380426

VL - 121

SP - 89

EP - 100

JO - Archives of Virology

JF - Archives of Virology

SN - 0304-8608

IS - 1-4

ER -