The role of electrical signals in murine corneal wound re-epithelialization

Romana Kucerova, Petr Walczysko, Brian Reid, Jingxing Ou, Lucy J. Leiper, Ann M. Rajnicek, Colin D. Mccaig, Min Zhao, J. Martin Collinson

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Ion flow from intact tissue into epithelial wound sites results in lateral electric currents that may represent a major driver of wound healing cell migration. Use of applied electric fields (EF) to promote wound healing is the basis of Medicare-approved electric stimulation therapy. This study investigated the roles for EFs in wound re-epithelialization, using the Pax6+/- mouse model of the human ocular surface abnormality aniridic keratopathy (in which wound healing and corneal epithelial cell migration are disrupted). Both wild-type (WT) and Pax6+/- corneal epithelial cells showed increased migration speeds in response to applied EFs in vitro. However, only Pax6+/+ cells demonstrated consistent directional galvanotaxis towards the cathode, with activation of pSrc signaling, polarized to the leading edges of cells. In vivo, the epithelial wound site normally represents a cathode, but 43% of Pax6+/- corneas exhibited reversed endogenous wound-induced currents (the wound was an anode). These corneas healed at the same rate as WT. Surprisingly, epithelial migration did not correlate with direction or magnitude of endogenous currents for WT or mutant corneas. Furthermore, during healing in vivo, no polarization of pSrc was observed. We found little evidence that Src-dependent mechanisms of cell migration, observed in response to applied EFs in vitro, normally exist in vivo. It is concluded that endogenous EFs do not drive long-term directionality of sustained healing migration in this mouse corneal epithelial model. Ion flow from wounds may nevertheless represent an important component of wound signaling initiation.

Original languageEnglish (US)
Pages (from-to)1544-1553
Number of pages10
JournalJournal of Cellular Physiology
Volume226
Issue number6
DOIs
StatePublished - Jun 2011

Fingerprint

Re-Epithelialization
Cathodes
Ions
Induced currents
Wounds and Injuries
Electric currents
Wound Healing
Cornea
Cell Movement
Anodes
Electrodes
Chemical activation
Electric fields
Polarization
Tissue
Epithelial Cells
Eye Abnormalities
Electric Stimulation Therapy
Medicare
Epithelium

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Kucerova, R., Walczysko, P., Reid, B., Ou, J., Leiper, L. J., Rajnicek, A. M., ... Collinson, J. M. (2011). The role of electrical signals in murine corneal wound re-epithelialization. Journal of Cellular Physiology, 226(6), 1544-1553. https://doi.org/10.1002/jcp.22488

The role of electrical signals in murine corneal wound re-epithelialization. / Kucerova, Romana; Walczysko, Petr; Reid, Brian; Ou, Jingxing; Leiper, Lucy J.; Rajnicek, Ann M.; Mccaig, Colin D.; Zhao, Min; Collinson, J. Martin.

In: Journal of Cellular Physiology, Vol. 226, No. 6, 06.2011, p. 1544-1553.

Research output: Contribution to journalArticle

Kucerova, R, Walczysko, P, Reid, B, Ou, J, Leiper, LJ, Rajnicek, AM, Mccaig, CD, Zhao, M & Collinson, JM 2011, 'The role of electrical signals in murine corneal wound re-epithelialization', Journal of Cellular Physiology, vol. 226, no. 6, pp. 1544-1553. https://doi.org/10.1002/jcp.22488
Kucerova R, Walczysko P, Reid B, Ou J, Leiper LJ, Rajnicek AM et al. The role of electrical signals in murine corneal wound re-epithelialization. Journal of Cellular Physiology. 2011 Jun;226(6):1544-1553. https://doi.org/10.1002/jcp.22488
Kucerova, Romana ; Walczysko, Petr ; Reid, Brian ; Ou, Jingxing ; Leiper, Lucy J. ; Rajnicek, Ann M. ; Mccaig, Colin D. ; Zhao, Min ; Collinson, J. Martin. / The role of electrical signals in murine corneal wound re-epithelialization. In: Journal of Cellular Physiology. 2011 ; Vol. 226, No. 6. pp. 1544-1553.
@article{143594d7c6024c2dadd16321e52dc7f6,
title = "The role of electrical signals in murine corneal wound re-epithelialization",
abstract = "Ion flow from intact tissue into epithelial wound sites results in lateral electric currents that may represent a major driver of wound healing cell migration. Use of applied electric fields (EF) to promote wound healing is the basis of Medicare-approved electric stimulation therapy. This study investigated the roles for EFs in wound re-epithelialization, using the Pax6+/- mouse model of the human ocular surface abnormality aniridic keratopathy (in which wound healing and corneal epithelial cell migration are disrupted). Both wild-type (WT) and Pax6+/- corneal epithelial cells showed increased migration speeds in response to applied EFs in vitro. However, only Pax6+/+ cells demonstrated consistent directional galvanotaxis towards the cathode, with activation of pSrc signaling, polarized to the leading edges of cells. In vivo, the epithelial wound site normally represents a cathode, but 43{\%} of Pax6+/- corneas exhibited reversed endogenous wound-induced currents (the wound was an anode). These corneas healed at the same rate as WT. Surprisingly, epithelial migration did not correlate with direction or magnitude of endogenous currents for WT or mutant corneas. Furthermore, during healing in vivo, no polarization of pSrc was observed. We found little evidence that Src-dependent mechanisms of cell migration, observed in response to applied EFs in vitro, normally exist in vivo. It is concluded that endogenous EFs do not drive long-term directionality of sustained healing migration in this mouse corneal epithelial model. Ion flow from wounds may nevertheless represent an important component of wound signaling initiation.",
author = "Romana Kucerova and Petr Walczysko and Brian Reid and Jingxing Ou and Leiper, {Lucy J.} and Rajnicek, {Ann M.} and Mccaig, {Colin D.} and Min Zhao and Collinson, {J. Martin}",
year = "2011",
month = "6",
doi = "10.1002/jcp.22488",
language = "English (US)",
volume = "226",
pages = "1544--1553",
journal = "Journal of Cellular Physiology",
issn = "0021-9541",
publisher = "Wiley-Liss Inc.",
number = "6",

}

TY - JOUR

T1 - The role of electrical signals in murine corneal wound re-epithelialization

AU - Kucerova, Romana

AU - Walczysko, Petr

AU - Reid, Brian

AU - Ou, Jingxing

AU - Leiper, Lucy J.

AU - Rajnicek, Ann M.

AU - Mccaig, Colin D.

AU - Zhao, Min

AU - Collinson, J. Martin

PY - 2011/6

Y1 - 2011/6

N2 - Ion flow from intact tissue into epithelial wound sites results in lateral electric currents that may represent a major driver of wound healing cell migration. Use of applied electric fields (EF) to promote wound healing is the basis of Medicare-approved electric stimulation therapy. This study investigated the roles for EFs in wound re-epithelialization, using the Pax6+/- mouse model of the human ocular surface abnormality aniridic keratopathy (in which wound healing and corneal epithelial cell migration are disrupted). Both wild-type (WT) and Pax6+/- corneal epithelial cells showed increased migration speeds in response to applied EFs in vitro. However, only Pax6+/+ cells demonstrated consistent directional galvanotaxis towards the cathode, with activation of pSrc signaling, polarized to the leading edges of cells. In vivo, the epithelial wound site normally represents a cathode, but 43% of Pax6+/- corneas exhibited reversed endogenous wound-induced currents (the wound was an anode). These corneas healed at the same rate as WT. Surprisingly, epithelial migration did not correlate with direction or magnitude of endogenous currents for WT or mutant corneas. Furthermore, during healing in vivo, no polarization of pSrc was observed. We found little evidence that Src-dependent mechanisms of cell migration, observed in response to applied EFs in vitro, normally exist in vivo. It is concluded that endogenous EFs do not drive long-term directionality of sustained healing migration in this mouse corneal epithelial model. Ion flow from wounds may nevertheless represent an important component of wound signaling initiation.

AB - Ion flow from intact tissue into epithelial wound sites results in lateral electric currents that may represent a major driver of wound healing cell migration. Use of applied electric fields (EF) to promote wound healing is the basis of Medicare-approved electric stimulation therapy. This study investigated the roles for EFs in wound re-epithelialization, using the Pax6+/- mouse model of the human ocular surface abnormality aniridic keratopathy (in which wound healing and corneal epithelial cell migration are disrupted). Both wild-type (WT) and Pax6+/- corneal epithelial cells showed increased migration speeds in response to applied EFs in vitro. However, only Pax6+/+ cells demonstrated consistent directional galvanotaxis towards the cathode, with activation of pSrc signaling, polarized to the leading edges of cells. In vivo, the epithelial wound site normally represents a cathode, but 43% of Pax6+/- corneas exhibited reversed endogenous wound-induced currents (the wound was an anode). These corneas healed at the same rate as WT. Surprisingly, epithelial migration did not correlate with direction or magnitude of endogenous currents for WT or mutant corneas. Furthermore, during healing in vivo, no polarization of pSrc was observed. We found little evidence that Src-dependent mechanisms of cell migration, observed in response to applied EFs in vitro, normally exist in vivo. It is concluded that endogenous EFs do not drive long-term directionality of sustained healing migration in this mouse corneal epithelial model. Ion flow from wounds may nevertheless represent an important component of wound signaling initiation.

UR - http://www.scopus.com/inward/record.url?scp=79952729250&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79952729250&partnerID=8YFLogxK

U2 - 10.1002/jcp.22488

DO - 10.1002/jcp.22488

M3 - Article

C2 - 20945376

AN - SCOPUS:79952729250

VL - 226

SP - 1544

EP - 1553

JO - Journal of Cellular Physiology

JF - Journal of Cellular Physiology

SN - 0021-9541

IS - 6

ER -