The role of atrial natriuretic peptide to attenuate inflammation in a mouse skin wound and individually perfused rat mesenteric microvessels

Fitz Roy E Curry, Joyce F. Clark, Yanyan Jiang, Min Ho Kim, Roger H. Adamson, Scott I. Simon

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We tested the hypothesis that the anti-inflammatory actions of atrial natriuretic peptide (ANP) result from the modulation of leukocyte adhesion to inflamed endothelium and not solely ANP ligation of endothelial receptors to stabilize endothelial barrier function. We measured vascular permeability to albumin and accumulation of fluorescent neutrophils in a full-thickness skin wound on the flank of LysM-EGFP mice 24 h after formation. Vascular permeability in individually perfused rat mesenteric microvessels was also measured after leukocytes were washed out of the vessel lumen. Thrombin increased albumin permeability and increased the accumulation of neutrophils. The thrombin-induced inflammatory responses were attenuated by pretreating the wound with ANP (30 min). During pretreatment ANP did not lower permeability, but transiently increased baseline albumin permeability concomitant with the reduction in neutrophil accumulation. ANP did not attenuate acute increases in permeability to histamine and bradykinin in individually perfused rat microvessels. The hypothesis that anti-inflammatory actions of ANP depend solely on endothelial responses that stabilize the endothelial barrier is not supported by our results in either individually perfused microvessels in the absence of circulating leukocytes or the more chronic skin wound model. Our results conform to the alternate hypothesis that ANP modulates the interaction of leukocytes with the inflamed microvascular wall of the 24 h wound. Taken together with our previous observations that ANP reduces deformability of neutrophils and their strength of attachment, rolling, and transvascular migration, these observations provide the basis for additional investigations of ANP as an anti-inflammatory agent to modulate leukocyte–endothelial cell interactions.

Original languageEnglish (US)
Article numbere12968
JournalPhysiological Reports
Volume4
Issue number18
DOIs
StatePublished - Sep 1 2016

Fingerprint

Atrial Natriuretic Factor
Microvessels
Inflammation
Skin
Wounds and Injuries
Permeability
Neutrophils
Leukocytes
Albumins
Anti-Inflammatory Agents
Capillary Permeability
Thrombin
Bradykinin
Cell Communication
Histamine
Endothelium
Ligation

Keywords

  • Individually perfused microvessels
  • leukocyte deformability
  • leukocyte–endothelial interaction
  • vascular permeability

ASJC Scopus subject areas

  • Physiology (medical)
  • Physiology

Cite this

The role of atrial natriuretic peptide to attenuate inflammation in a mouse skin wound and individually perfused rat mesenteric microvessels. / Curry, Fitz Roy E; Clark, Joyce F.; Jiang, Yanyan; Kim, Min Ho; Adamson, Roger H.; Simon, Scott I.

In: Physiological Reports, Vol. 4, No. 18, e12968, 01.09.2016.

Research output: Contribution to journalArticle

Curry, Fitz Roy E ; Clark, Joyce F. ; Jiang, Yanyan ; Kim, Min Ho ; Adamson, Roger H. ; Simon, Scott I. / The role of atrial natriuretic peptide to attenuate inflammation in a mouse skin wound and individually perfused rat mesenteric microvessels. In: Physiological Reports. 2016 ; Vol. 4, No. 18.
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