Abstract
We have examined the constitutive and induced expression of As60A in Anopheles stephensi females. As60A is expressed throughout the body of A. stephensi, including the midgut, fat body and developing eggs. We discovered that As60A is induced in the midgut and carcass of A. stephensi in response to Plasmodium infection. Induction of As60A correlates with periods of parasite motility and reproduction. Further, induction is dependent on the intensity of parasite infection: low numbers of parasites do not induce As60A expression. Thus, we conclude that As60A is a component of the A. stephensi immune response to Plasmodium infection. The involvement of a member of the transforming growth factor beta (TGF-β) super family in the mosquito immune response is analogous to the involvement of TGF-β1 in the mammalian immune response to Plasmodium. The modulation of As60A and A. stephensi nitric oxide synthase (AsNOS) expression in response to Plasmodium indicates that homologs of effector (NOS) and regulator (TGF-β1) gene super families may defend evolutionarily diverse hosts againsts a shared pathogen.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 131-141 |
| Number of pages | 11 |
| Journal | Infection, Genetics and Evolution |
| Volume | 1 |
| Issue number | 2 |
| DOIs | |
| State | Published - 2001 |
| Externally published | Yes |
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Keywords
- As60A
- Innate immunity
- Malaria
- Mosquito
- Plasmodium
- Transforming growth factor beta (TGF-β)
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Genetics
- Microbiology
- Infectious Diseases
Cite this
The role of As60A, a TGF-β homolog, in Anopheles stephensi innate immunity and defense against Plasmodium infection. / Crampton, Andrea; Luckhart, Shirley.
In: Infection, Genetics and Evolution, Vol. 1, No. 2, 2001, p. 131-141.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The role of As60A, a TGF-β homolog, in Anopheles stephensi innate immunity and defense against Plasmodium infection
AU - Crampton, Andrea
AU - Luckhart, Shirley
PY - 2001
Y1 - 2001
N2 - We have examined the constitutive and induced expression of As60A in Anopheles stephensi females. As60A is expressed throughout the body of A. stephensi, including the midgut, fat body and developing eggs. We discovered that As60A is induced in the midgut and carcass of A. stephensi in response to Plasmodium infection. Induction of As60A correlates with periods of parasite motility and reproduction. Further, induction is dependent on the intensity of parasite infection: low numbers of parasites do not induce As60A expression. Thus, we conclude that As60A is a component of the A. stephensi immune response to Plasmodium infection. The involvement of a member of the transforming growth factor beta (TGF-β) super family in the mosquito immune response is analogous to the involvement of TGF-β1 in the mammalian immune response to Plasmodium. The modulation of As60A and A. stephensi nitric oxide synthase (AsNOS) expression in response to Plasmodium indicates that homologs of effector (NOS) and regulator (TGF-β1) gene super families may defend evolutionarily diverse hosts againsts a shared pathogen.
AB - We have examined the constitutive and induced expression of As60A in Anopheles stephensi females. As60A is expressed throughout the body of A. stephensi, including the midgut, fat body and developing eggs. We discovered that As60A is induced in the midgut and carcass of A. stephensi in response to Plasmodium infection. Induction of As60A correlates with periods of parasite motility and reproduction. Further, induction is dependent on the intensity of parasite infection: low numbers of parasites do not induce As60A expression. Thus, we conclude that As60A is a component of the A. stephensi immune response to Plasmodium infection. The involvement of a member of the transforming growth factor beta (TGF-β) super family in the mosquito immune response is analogous to the involvement of TGF-β1 in the mammalian immune response to Plasmodium. The modulation of As60A and A. stephensi nitric oxide synthase (AsNOS) expression in response to Plasmodium indicates that homologs of effector (NOS) and regulator (TGF-β1) gene super families may defend evolutionarily diverse hosts againsts a shared pathogen.
KW - As60A
KW - Innate immunity
KW - Malaria
KW - Mosquito
KW - Plasmodium
KW - Transforming growth factor beta (TGF-β)
UR - http://www.scopus.com/inward/record.url?scp=0035683921&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035683921&partnerID=8YFLogxK
U2 - 10.1016/S1567-1348(01)00017-X
DO - 10.1016/S1567-1348(01)00017-X
M3 - Article
C2 - 12798028
AN - SCOPUS:0035683921
VL - 1
SP - 131
EP - 141
JO - Infection, Genetics and Evolution
JF - Infection, Genetics and Evolution
SN - 1567-1348
IS - 2
ER -