The role of As60A, a TGF-β homolog, in Anopheles stephensi innate immunity and defense against Plasmodium infection

Andrea Crampton, Shirley Luckhart

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We have examined the constitutive and induced expression of As60A in Anopheles stephensi females. As60A is expressed throughout the body of A. stephensi, including the midgut, fat body and developing eggs. We discovered that As60A is induced in the midgut and carcass of A. stephensi in response to Plasmodium infection. Induction of As60A correlates with periods of parasite motility and reproduction. Further, induction is dependent on the intensity of parasite infection: low numbers of parasites do not induce As60A expression. Thus, we conclude that As60A is a component of the A. stephensi immune response to Plasmodium infection. The involvement of a member of the transforming growth factor beta (TGF-β) super family in the mosquito immune response is analogous to the involvement of TGF-β1 in the mammalian immune response to Plasmodium. The modulation of As60A and A. stephensi nitric oxide synthase (AsNOS) expression in response to Plasmodium indicates that homologs of effector (NOS) and regulator (TGF-β1) gene super families may defend evolutionarily diverse hosts againsts a shared pathogen.

Original languageEnglish (US)
Pages (from-to)131-141
Number of pages11
JournalInfection, Genetics and Evolution
Volume1
Issue number2
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Anopheles stephensi
Anopheles
transforming growth factor beta
Plasmodium
immunity
immune response
Innate Immunity
Transforming Growth Factor beta
Malaria
parasite
transforming growth factor beta 1
Parasites
infection
Parasitic Diseases
Fat Body
growth regulator
parasites
midgut
motility
nitric oxide

Keywords

  • As60A
  • Innate immunity
  • Malaria
  • Mosquito
  • Plasmodium
  • Transforming growth factor beta (TGF-β)

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Microbiology
  • Infectious Diseases

Cite this

The role of As60A, a TGF-β homolog, in Anopheles stephensi innate immunity and defense against Plasmodium infection. / Crampton, Andrea; Luckhart, Shirley.

In: Infection, Genetics and Evolution, Vol. 1, No. 2, 2001, p. 131-141.

Research output: Contribution to journalArticle

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