The role of 16S rRNA gene sequencing in identification of microorganisms misidentified by conventional methods

C. A. Petti, Christopher R Polage, P. Schreckenberger

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

Traditional methods for microbial identification require the recognition of differences in morphology, growth, enzymatic activity, and metabolism to define genera and species. Full and partial 16S rRNA gene sequencing methods have emerged as useful tools for identifying phenotypically aberrant microorganisms. We report on three bacterial blood isolates from three different College of American Pathologists-certified laboratories that were referred to ARUP Laboratories for definitive identification. Because phenotypic identification suggested unusual organisms not typically associated with the submitted clinical diagnosis, consultation with the Medical Director was sought and further testing was performed including partial 16S rRNA gene sequencing. All three patients had endocarditis, and conventional methods identified isolates from patients A, B, and C as a Facklamia sp., Eubacterium tenue, and a Bifidobacterium sp. 16S rRNA gene sequencing identified the isolates as Enterococcus faecalis, Cardiobacterium valvarum, and Streptococcus mutans, respectively. We conclude that the initial identifications of these three isolates were erroneous, may have misled clinicians, and potentially impacted patient care. 16S rRNA gene sequencing is a more objective identification tool, unaffected by phenotypic variation or technologist bias, and has the potential to reduce laboratory errors.

Original languageEnglish (US)
Pages (from-to)6123-6125
Number of pages3
JournalJournal of Clinical Microbiology
Volume43
Issue number12
DOIs
StatePublished - Dec 2005
Externally publishedYes

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rRNA Genes
Cardiobacterium
Eubacterium
Physician Executives
Bifidobacterium
Streptococcus mutans
Enterococcus faecalis
Endocarditis
Patient Care
Referral and Consultation
Growth

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

The role of 16S rRNA gene sequencing in identification of microorganisms misidentified by conventional methods. / Petti, C. A.; Polage, Christopher R; Schreckenberger, P.

In: Journal of Clinical Microbiology, Vol. 43, No. 12, 12.2005, p. 6123-6125.

Research output: Contribution to journalArticle

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