The risk of nosocomial pneumonia is not increased during partial liquid ventilation

Imran Sajan, Frank A. Scannapieco, Bradley P. Fuhrman, David M. Steinhorn

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Objective: To determine whether partial liquid ventilation (PLV) affects the risk of nosocomial pneumonia. Study Design: To assess in vitro bacterial adhesion and viability after liquid perfluorocarbon exposure and to assess bacterial recovery after partial liquid ventilation in vivo in rabbits. Setting: University animal research facility. Subjects: Thirty-six New Zealand White rabbits. Interventions: To assess adhesions, radiolabeled Escherichia coli were exposed to perfluorocarbon, incubated against artificial biosurfaces, and compared with nonexposed controls. Bacterial viability in vitro was assessed by exposing broth suspensions of Pasteurella multocida to perflubron for various times. Controls were run in parallel without exposure. Quantitative cultures were performed to determine viability. We undertook short-term and recovery in vivo investigations. The lungs of treated animals were filled with perflubron (~18 mL/kg), and the control rabbits were ventilated without perflubron in an identical fashion. Cryopreserved aliquots of P. multocida were administered via an endotracheal tube. The short-term study animals were ventilated for 6 hrs before being killed. The recovery animals were ventilated for 2-4 hrs, extubated, and killed 20 hrs later. The lungs were removed, aseptically minced, and homogenized. Serial dilutions of the homogenate were quantitatively cultured by manual counting of colonies on agar plates. The recovered organisms were typed for species by the clinical microbiology laboratory. Measurements and Main Results: The adhesion of bacteria to immobilized bronchoalveolar lavage and human saliva, respectively, was reduced by 65% ± 7% and 66% ± 1% (p < .05; n = 5) after exposure to perflubron and by 63% ± 9% and 68% ± 6% after exposure to FC-77 (p < .05; n = 5); however, adhesion was not affected by exposure to Rimar. There was no difference in bacterial viability between the control and perflubron-exposed bacteria (n = 5). The in vivo study demonstrated a ten-fold or greater reduction in the number of recovered bacteria in the partial liquid ventilated group compared with the control group. Conclusions: This study suggests that different perfluorocarbons affect adhesions differently. Perflubron and FC-77 appear to decrease bacterial adhesion, whereas Rimar does not. Rerflubron does not have a direct bactericidal effect. Furthermore, PLV with perflubron decreased the number of viable bacteria per gram of tissue after an intentional inoculation of the airway, suggesting that the risk of nosocomial pneumonia is unlikely to be increased during PLV and may, in fact, be reduced in patients supported with PLV.

Original languageEnglish (US)
Pages (from-to)2741-2747
Number of pages7
JournalCritical Care Medicine
Volume27
Issue number12
StatePublished - 1999
Externally publishedYes

Fingerprint

Liquid Ventilation
Pneumonia
Microbial Viability
Fluorocarbons
Bacterial Adhesion
Bacteria
Pasteurella multocida
Rabbits
Lung
Bronchoalveolar Lavage
Microbiology
perflubron
Saliva
Agar
Suspensions
Escherichia coli
Control Groups

Keywords

  • Acute lung injury
  • Adult respiratory distress syndrome
  • Bacterial pneumonia
  • Liquid ventilation
  • Mechanical ventilation
  • Nosocomial pneumonia
  • Partial liquid ventilation
  • Pasteurella multocida
  • Perflubron
  • Perfluorocarbon
  • Perfluorooctylbromide

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

Cite this

Sajan, I., Scannapieco, F. A., Fuhrman, B. P., & Steinhorn, D. M. (1999). The risk of nosocomial pneumonia is not increased during partial liquid ventilation. Critical Care Medicine, 27(12), 2741-2747.

The risk of nosocomial pneumonia is not increased during partial liquid ventilation. / Sajan, Imran; Scannapieco, Frank A.; Fuhrman, Bradley P.; Steinhorn, David M.

In: Critical Care Medicine, Vol. 27, No. 12, 1999, p. 2741-2747.

Research output: Contribution to journalArticle

Sajan, I, Scannapieco, FA, Fuhrman, BP & Steinhorn, DM 1999, 'The risk of nosocomial pneumonia is not increased during partial liquid ventilation', Critical Care Medicine, vol. 27, no. 12, pp. 2741-2747.
Sajan I, Scannapieco FA, Fuhrman BP, Steinhorn DM. The risk of nosocomial pneumonia is not increased during partial liquid ventilation. Critical Care Medicine. 1999;27(12):2741-2747.
Sajan, Imran ; Scannapieco, Frank A. ; Fuhrman, Bradley P. ; Steinhorn, David M. / The risk of nosocomial pneumonia is not increased during partial liquid ventilation. In: Critical Care Medicine. 1999 ; Vol. 27, No. 12. pp. 2741-2747.
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abstract = "Objective: To determine whether partial liquid ventilation (PLV) affects the risk of nosocomial pneumonia. Study Design: To assess in vitro bacterial adhesion and viability after liquid perfluorocarbon exposure and to assess bacterial recovery after partial liquid ventilation in vivo in rabbits. Setting: University animal research facility. Subjects: Thirty-six New Zealand White rabbits. Interventions: To assess adhesions, radiolabeled Escherichia coli were exposed to perfluorocarbon, incubated against artificial biosurfaces, and compared with nonexposed controls. Bacterial viability in vitro was assessed by exposing broth suspensions of Pasteurella multocida to perflubron for various times. Controls were run in parallel without exposure. Quantitative cultures were performed to determine viability. We undertook short-term and recovery in vivo investigations. The lungs of treated animals were filled with perflubron (~18 mL/kg), and the control rabbits were ventilated without perflubron in an identical fashion. Cryopreserved aliquots of P. multocida were administered via an endotracheal tube. The short-term study animals were ventilated for 6 hrs before being killed. The recovery animals were ventilated for 2-4 hrs, extubated, and killed 20 hrs later. The lungs were removed, aseptically minced, and homogenized. Serial dilutions of the homogenate were quantitatively cultured by manual counting of colonies on agar plates. The recovered organisms were typed for species by the clinical microbiology laboratory. Measurements and Main Results: The adhesion of bacteria to immobilized bronchoalveolar lavage and human saliva, respectively, was reduced by 65{\%} ± 7{\%} and 66{\%} ± 1{\%} (p < .05; n = 5) after exposure to perflubron and by 63{\%} ± 9{\%} and 68{\%} ± 6{\%} after exposure to FC-77 (p < .05; n = 5); however, adhesion was not affected by exposure to Rimar. There was no difference in bacterial viability between the control and perflubron-exposed bacteria (n = 5). The in vivo study demonstrated a ten-fold or greater reduction in the number of recovered bacteria in the partial liquid ventilated group compared with the control group. Conclusions: This study suggests that different perfluorocarbons affect adhesions differently. Perflubron and FC-77 appear to decrease bacterial adhesion, whereas Rimar does not. Rerflubron does not have a direct bactericidal effect. Furthermore, PLV with perflubron decreased the number of viable bacteria per gram of tissue after an intentional inoculation of the airway, suggesting that the risk of nosocomial pneumonia is unlikely to be increased during PLV and may, in fact, be reduced in patients supported with PLV.",
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AU - Sajan, Imran

AU - Scannapieco, Frank A.

AU - Fuhrman, Bradley P.

AU - Steinhorn, David M.

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N2 - Objective: To determine whether partial liquid ventilation (PLV) affects the risk of nosocomial pneumonia. Study Design: To assess in vitro bacterial adhesion and viability after liquid perfluorocarbon exposure and to assess bacterial recovery after partial liquid ventilation in vivo in rabbits. Setting: University animal research facility. Subjects: Thirty-six New Zealand White rabbits. Interventions: To assess adhesions, radiolabeled Escherichia coli were exposed to perfluorocarbon, incubated against artificial biosurfaces, and compared with nonexposed controls. Bacterial viability in vitro was assessed by exposing broth suspensions of Pasteurella multocida to perflubron for various times. Controls were run in parallel without exposure. Quantitative cultures were performed to determine viability. We undertook short-term and recovery in vivo investigations. The lungs of treated animals were filled with perflubron (~18 mL/kg), and the control rabbits were ventilated without perflubron in an identical fashion. Cryopreserved aliquots of P. multocida were administered via an endotracheal tube. The short-term study animals were ventilated for 6 hrs before being killed. The recovery animals were ventilated for 2-4 hrs, extubated, and killed 20 hrs later. The lungs were removed, aseptically minced, and homogenized. Serial dilutions of the homogenate were quantitatively cultured by manual counting of colonies on agar plates. The recovered organisms were typed for species by the clinical microbiology laboratory. Measurements and Main Results: The adhesion of bacteria to immobilized bronchoalveolar lavage and human saliva, respectively, was reduced by 65% ± 7% and 66% ± 1% (p < .05; n = 5) after exposure to perflubron and by 63% ± 9% and 68% ± 6% after exposure to FC-77 (p < .05; n = 5); however, adhesion was not affected by exposure to Rimar. There was no difference in bacterial viability between the control and perflubron-exposed bacteria (n = 5). The in vivo study demonstrated a ten-fold or greater reduction in the number of recovered bacteria in the partial liquid ventilated group compared with the control group. Conclusions: This study suggests that different perfluorocarbons affect adhesions differently. Perflubron and FC-77 appear to decrease bacterial adhesion, whereas Rimar does not. Rerflubron does not have a direct bactericidal effect. Furthermore, PLV with perflubron decreased the number of viable bacteria per gram of tissue after an intentional inoculation of the airway, suggesting that the risk of nosocomial pneumonia is unlikely to be increased during PLV and may, in fact, be reduced in patients supported with PLV.

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KW - Adult respiratory distress syndrome

KW - Bacterial pneumonia

KW - Liquid ventilation

KW - Mechanical ventilation

KW - Nosocomial pneumonia

KW - Partial liquid ventilation

KW - Pasteurella multocida

KW - Perflubron

KW - Perfluorocarbon

KW - Perfluorooctylbromide

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