The response of arabidopsis to co-cultivation with clover

Investigating plant-plant interactions with metabolomics

Hans A. Pedersen, Per Kudsk, Oliver Fiehn, Inge S. Fomsgaard

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Allelopathy contributes to interspecific interference in plants beyond competition for nutrients and sunlight and is of interest in agriculture due to its potential use in weed suppression. In order to study allelopathic effects in a model system, Arabidopsis thaliana and Trifolium repens were co-cultivated on nutrient medium in sterile containers for two weeks and then harvested, extracted and analyzed by GC-TOF-MS. 163 metabolites were identified using the automated database BinBase. Comparing metabolite peak areas in co-cultivated and control seedlings revealed an altered metabolic profile for both species in terms of several metabolite groups including amino acids, phenolics, carbohydrates and lipids. 87 A. thaliana and 53 T. repens metabolites were significantly affected. In A. thaliana 34 metabolites increased relative to the control upon co-cultivation while 54 decreased; in T. repens 20 increased while 33 decreased. To our knowledge, this is the first plant-plant interaction study making use of untargeted library-based metabolomics. The observed decreases in A. thaliana of aromatic and branched-chain amino acids suggest a suppressant effect of T. repens on A. thaliana which may resemble that of herbicides, as the synthesis of these two groups of amino acids is inhibited by glyphosate and sulfonylurea herbicides respectively. While the results are not conclusive, they illustrate the power of untargeted library-based metabolomics in descriptive studies and in generating hypotheses for further study.

Original languageEnglish (US)
Title of host publicationACS Symposium Series
PublisherAmerican Chemical Society
Pages189-201
Number of pages13
Volume1141
ISBN (Print)9780841229006
DOIs
StatePublished - 2013

Publication series

NameACS Symposium Series
Volume1141
ISSN (Print)00976156
ISSN (Electronic)19475918

Fingerprint

Metabolites
Amino acids
glyphosate
Herbicides
Nutrients
Amino Acids
Branched Chain Amino Acids
Carbohydrates
Agriculture
Lipids
Containers
Metabolomics

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Pedersen, H. A., Kudsk, P., Fiehn, O., & Fomsgaard, I. S. (2013). The response of arabidopsis to co-cultivation with clover: Investigating plant-plant interactions with metabolomics. In ACS Symposium Series (Vol. 1141, pp. 189-201). (ACS Symposium Series; Vol. 1141). American Chemical Society. https://doi.org/10.1021/bk-2013-1141.ch013

The response of arabidopsis to co-cultivation with clover : Investigating plant-plant interactions with metabolomics. / Pedersen, Hans A.; Kudsk, Per; Fiehn, Oliver; Fomsgaard, Inge S.

ACS Symposium Series. Vol. 1141 American Chemical Society, 2013. p. 189-201 (ACS Symposium Series; Vol. 1141).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Pedersen, HA, Kudsk, P, Fiehn, O & Fomsgaard, IS 2013, The response of arabidopsis to co-cultivation with clover: Investigating plant-plant interactions with metabolomics. in ACS Symposium Series. vol. 1141, ACS Symposium Series, vol. 1141, American Chemical Society, pp. 189-201. https://doi.org/10.1021/bk-2013-1141.ch013
Pedersen HA, Kudsk P, Fiehn O, Fomsgaard IS. The response of arabidopsis to co-cultivation with clover: Investigating plant-plant interactions with metabolomics. In ACS Symposium Series. Vol. 1141. American Chemical Society. 2013. p. 189-201. (ACS Symposium Series). https://doi.org/10.1021/bk-2013-1141.ch013
Pedersen, Hans A. ; Kudsk, Per ; Fiehn, Oliver ; Fomsgaard, Inge S. / The response of arabidopsis to co-cultivation with clover : Investigating plant-plant interactions with metabolomics. ACS Symposium Series. Vol. 1141 American Chemical Society, 2013. pp. 189-201 (ACS Symposium Series).
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