The regulatory functions of a new tetrapeptide from the bursa of Fabricius on AIV vaccine immunization and antibody production

Man Man Zong, Chuan Jie Zhou, Yuan Nan Yu, Yang Zheng, Xiuli Feng, Pu Yan Chen, Mei Yang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Understanding the regulatory functions of the biological peptide from the humoral central immune organ bursa of Fabricius on vaccine immune responses and antibody production is of vital importance. Objectives: Here we thoroughly verified the immunomodulatory functions of the new tetrapeptide BP4 from the bursa of Fabricius on vaccine immune responses in mice and chicken immunizaiton model, and on potential intracellular signaling during antibody production. Method: BP4 was isolated and identified by Reverse Phase High Performance Liquid Chromatography and matrix-assisted laser desorption ionization time of flight mass spectrometry. immunomodulatory functions of BP4 was verified by AIV vaccine immunization on mice and chickens regarding roles in vivo, by monitoring the impact of signalling inhibitors in hybridoma cells on antibody production in vitro. Results: Our investigation revealed the strong inducing roles of new isolated BP4 on immune responses in mice immunization, the immunomodulatory effects in the immunized chicken, four potential key intracellular signaling during antibody production in hybrdoma cells. Conclusion: The new bursal-derived peptide BP4 was isolated and identified, and the immunomodulatory effects on antigen-specific immune responses in vivo and in vitro were verified, suggesting BP4 might be highly relevant to the humoral immune responses, and PI3K/Akt, p38 MAPK, NF-κB and tyrosine phosphorylation signaling might be the key activated intracellular signaling during antibody production during BP4 stimulation, which provided a novel potential adjuvant candidate for vaccine immunization improvement and precaution on animal epidemic disease.

Original languageEnglish (US)
Pages (from-to)582-589
Number of pages8
JournalProtein and Peptide Letters
Volume24
Issue number7
DOIs
StatePublished - Jan 1 2017
Externally publishedYes

Fingerprint

Bursa of Fabricius
Immunization
Antibody Formation
Vaccines
Antibodies
Chickens
Animal Diseases
Peptides
Phosphorylation
Histocompatibility Antigens Class II
Hybridomas
High performance liquid chromatography
p38 Mitogen-Activated Protein Kinases
Reverse-Phase Chromatography
Humoral Immunity
Phosphatidylinositol 3-Kinases
Ionization
Mass spectrometry
Tyrosine
Desorption

Keywords

  • Antibody production
  • Bursal tetrapeptide
  • Hybridoma
  • Immune response
  • Immunization model
  • Signal pathways

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry

Cite this

The regulatory functions of a new tetrapeptide from the bursa of Fabricius on AIV vaccine immunization and antibody production. / Zong, Man Man; Zhou, Chuan Jie; Yu, Yuan Nan; Zheng, Yang; Feng, Xiuli; Chen, Pu Yan; Yang, Mei.

In: Protein and Peptide Letters, Vol. 24, No. 7, 01.01.2017, p. 582-589.

Research output: Contribution to journalArticle

Zong, Man Man ; Zhou, Chuan Jie ; Yu, Yuan Nan ; Zheng, Yang ; Feng, Xiuli ; Chen, Pu Yan ; Yang, Mei. / The regulatory functions of a new tetrapeptide from the bursa of Fabricius on AIV vaccine immunization and antibody production. In: Protein and Peptide Letters. 2017 ; Vol. 24, No. 7. pp. 582-589.
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