The rec102 mutant of yeast is defective in meiotic recombination and chromosome synapsis

J. Bhargava, J. Engebrecht, G. S. Roeder

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

A mutation at the REC102 locus was identified in a screen for yeast mutants that produce inviable spores. rec102 spore lethality is rescued by a spo13 mutation, which causes cells to bypass the meiosis I division. The rec102 mutation completely eliminates meiotically induced gene conversion and crossing over but has no effect on mitotic recombination frequencies. Cytological studies indicate that the rec102 mutant makes axial elements (precursors to the synaptonemal complex), but homologous chromosomes fail to synapse. In addition, meiotic chromosome segregation is significantly delayed in rec102 strains. Studies of double and triple mutants indicate that the REC102 protein acts before the RAD52 gene product in the meiotic recombination pathway. The REC102 gene was cloned based on complementation of the mutant defect and the gene was mapped to chromosome XII between CDC25 and STE11.

Original languageEnglish (US)
Pages (from-to)59-69
Number of pages11
JournalGenetics
Volume130
Issue number1
StatePublished - 1992
Externally publishedYes

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Chromosome Pairing
Genetic Recombination
Chromosomes
Yeasts
Spores
Mutation
Synaptonemal Complex
Genes
Gene Conversion
Chromosome Segregation
Meiosis
Synapses
Proteins

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Bhargava, J., Engebrecht, J., & Roeder, G. S. (1992). The rec102 mutant of yeast is defective in meiotic recombination and chromosome synapsis. Genetics, 130(1), 59-69.

The rec102 mutant of yeast is defective in meiotic recombination and chromosome synapsis. / Bhargava, J.; Engebrecht, J.; Roeder, G. S.

In: Genetics, Vol. 130, No. 1, 1992, p. 59-69.

Research output: Contribution to journalArticle

Bhargava, J, Engebrecht, J & Roeder, GS 1992, 'The rec102 mutant of yeast is defective in meiotic recombination and chromosome synapsis', Genetics, vol. 130, no. 1, pp. 59-69.
Bhargava, J. ; Engebrecht, J. ; Roeder, G. S. / The rec102 mutant of yeast is defective in meiotic recombination and chromosome synapsis. In: Genetics. 1992 ; Vol. 130, No. 1. pp. 59-69.
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