The reaction of nitric oxide with ubiquinol: Kinetic properties and biological significance

Juan José Poderoso, María Cecilia Carreras, Francisco Schöpfer, Constanza L. Lisdero, Natalia A. Riobó, Cecilia R Giulivi, Alejandro D. Boveris, Alberto Boveris, Enrique Cadenas

Research output: Contribution to journalArticle

138 Scopus citations

Abstract

The reaction of nitric oxide (·NO) with ubiquinol-0 and ubiquinol-2, short-chain analogs of coenzyme Q, was examined in anaerobic and aerobic conditions in terms of formation of intermediates and stable molecular products. The chemical reactivity of ubiquinol-0 and ubiquinol-2 towards ·NO differed only quantitatively, the reactions of ubiquinol-2 being slightly faster than those of ubiquinol-0. The ubiquinol/·NO reaction entailed oxidation of ubiquinol to ubiquinone and reduction of ·NO to NO-, the latter identified by its reaction with metmyoglobin to form nitroxylmyoglobin and indirectly by measurement of nitrous oxide (N2O) by gas chromatography. Both the rate of ubiquinone accumulation and ·NO consumption were linearly dependent on ubiquinol and ·NO concentrations. The stoichiometry of ·NO consumed per either ubiquinone formed or ubiquinol oxidized was 1.86 Å 0.34. The reaction of ·NO with ubiquinols proceeded with intermediate formation of ubisemiquinones that were detected by direct EPR. The second order rate constants of the reactions of ubiquinol-0 and ubiquinol-2 with ·NO were 0.49 and 1.6 x 104 M-1s-1, respectively. Studies in aerobic conditions revealed that the reaction of ·NO with ubiquinols was associated with O2 consumption. The formation of oxyradicals identified by spin trapping EPR during ubiquinol autoxidation was inhibited by ·NO, thus indicating that the O2 consumption triggered by ·NO could not be directly accounted for in terms of oxyradical formation or H2O2 accumulation. It is suggested that oxyradical formation is inhibited by the rapid removal of superoxide anion by ·NO to yield peroxynitrite, which subsequently may be involved in the propagation of ubiquinol oxidation. The biological significance of the reaction of ubiquinols with ·NO is discussed in terms of the cellular O2 gradients, the steady-state levels of ubiquinols and ·NO, and the distribution of ubiquinone (largely in its reduced form) in biological membranes with emphasis on the inner mitochondrial membrane.

Original languageEnglish (US)
Pages (from-to)925-935
Number of pages11
JournalFree Radical Biology and Medicine
Volume26
Issue number7-8
DOIs
StatePublished - Apr 1999
Externally publishedYes

Keywords

  • Antioxidants
  • Coenzyme Q
  • Free radical
  • Nitric oxide
  • Oxyradicals
  • Peroxynitrite
  • Ubiquinol
  • Ubiquinone

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry

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  • Cite this

    Poderoso, J. J., Carreras, M. C., Schöpfer, F., Lisdero, C. L., Riobó, N. A., Giulivi, C. R., Boveris, A. D., Boveris, A., & Cadenas, E. (1999). The reaction of nitric oxide with ubiquinol: Kinetic properties and biological significance. Free Radical Biology and Medicine, 26(7-8), 925-935. https://doi.org/10.1016/S0891-5849(98)00277-9