The putative E5 open reading frame of cottontail rabbit papillomavirus is dispensable for papilloma formation in domestic rabbits

Janet L. Brandsma, Zhi Hua Yang, Daniel DiMaio, Stephen W Barthold, Elizabeth Johnson, Wei Xiao

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

In the cottontail rabbit papillomavirus (CRPV)-rabbit system, recombinant CRPV DNA can induce papillomas. This investigation was undertaken to evaluate whether the E5 open reading frame (ORF) of CRPV is required for papilloma formation. The CPRV genome we utilized, CRPV-WA, was sequenced in the E5 region and was found to contain one deletion, two insertions, and one transition mutation compared with CRPV-KS, the CRPN genome that has been fully sequenced. Despite these differences, an intact E5 ORF is preserved, supporting the notion that this gene may serve a biological function. One frameshift and two in-frame mutations were constructed in the small region of the 5′ end of the E5 ORF that follows the E2 stop codon and precedes the L2 ORF. Several hundred rabbit skin sites were inoculated with each DNA preparation with a jet injector to test the ability of three CRPV E5 mutant DNAs to induce papillomas. In vivo results showed that each of the mutants induced papillomas, and biochemical analysis demonstrated that the E5 mutations present in DNA inocula were retained in the papillomas. The frequency of papilloma formation, however, was generally lower with each of the CRPV E5 mutants than with wiid-type CRPV DNA, particularly so for the E5 frameshift mutant, suggesting that although the recognized E5 ORF is not required in domestic rabbits for the induction of papillomas by CRPV DNA, it may facilitate their formation.

Original languageEnglish (US)
Pages (from-to)6204-6207
Number of pages4
JournalJournal of Virology
Volume66
Issue number10
StatePublished - Oct 1992
Externally publishedYes

Fingerprint

Cottontail rabbit papillomavirus
papilloma
Papilloma
Open Reading Frames
open reading frames
rabbits
Rabbits
DNA
mutants
mutation
Mutation
Genome
injectors
genome
Terminator Codon
stop codon
skin (animal)
inoculum

ASJC Scopus subject areas

  • Immunology

Cite this

The putative E5 open reading frame of cottontail rabbit papillomavirus is dispensable for papilloma formation in domestic rabbits. / Brandsma, Janet L.; Yang, Zhi Hua; DiMaio, Daniel; Barthold, Stephen W; Johnson, Elizabeth; Xiao, Wei.

In: Journal of Virology, Vol. 66, No. 10, 10.1992, p. 6204-6207.

Research output: Contribution to journalArticle

Brandsma, Janet L. ; Yang, Zhi Hua ; DiMaio, Daniel ; Barthold, Stephen W ; Johnson, Elizabeth ; Xiao, Wei. / The putative E5 open reading frame of cottontail rabbit papillomavirus is dispensable for papilloma formation in domestic rabbits. In: Journal of Virology. 1992 ; Vol. 66, No. 10. pp. 6204-6207.
@article{c93dfcaec9fc423f97d4efefe726e559,
title = "The putative E5 open reading frame of cottontail rabbit papillomavirus is dispensable for papilloma formation in domestic rabbits",
abstract = "In the cottontail rabbit papillomavirus (CRPV)-rabbit system, recombinant CRPV DNA can induce papillomas. This investigation was undertaken to evaluate whether the E5 open reading frame (ORF) of CRPV is required for papilloma formation. The CPRV genome we utilized, CRPV-WA, was sequenced in the E5 region and was found to contain one deletion, two insertions, and one transition mutation compared with CRPV-KS, the CRPN genome that has been fully sequenced. Despite these differences, an intact E5 ORF is preserved, supporting the notion that this gene may serve a biological function. One frameshift and two in-frame mutations were constructed in the small region of the 5′ end of the E5 ORF that follows the E2 stop codon and precedes the L2 ORF. Several hundred rabbit skin sites were inoculated with each DNA preparation with a jet injector to test the ability of three CRPV E5 mutant DNAs to induce papillomas. In vivo results showed that each of the mutants induced papillomas, and biochemical analysis demonstrated that the E5 mutations present in DNA inocula were retained in the papillomas. The frequency of papilloma formation, however, was generally lower with each of the CRPV E5 mutants than with wiid-type CRPV DNA, particularly so for the E5 frameshift mutant, suggesting that although the recognized E5 ORF is not required in domestic rabbits for the induction of papillomas by CRPV DNA, it may facilitate their formation.",
author = "Brandsma, {Janet L.} and Yang, {Zhi Hua} and Daniel DiMaio and Barthold, {Stephen W} and Elizabeth Johnson and Wei Xiao",
year = "1992",
month = "10",
language = "English (US)",
volume = "66",
pages = "6204--6207",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "10",

}

TY - JOUR

T1 - The putative E5 open reading frame of cottontail rabbit papillomavirus is dispensable for papilloma formation in domestic rabbits

AU - Brandsma, Janet L.

AU - Yang, Zhi Hua

AU - DiMaio, Daniel

AU - Barthold, Stephen W

AU - Johnson, Elizabeth

AU - Xiao, Wei

PY - 1992/10

Y1 - 1992/10

N2 - In the cottontail rabbit papillomavirus (CRPV)-rabbit system, recombinant CRPV DNA can induce papillomas. This investigation was undertaken to evaluate whether the E5 open reading frame (ORF) of CRPV is required for papilloma formation. The CPRV genome we utilized, CRPV-WA, was sequenced in the E5 region and was found to contain one deletion, two insertions, and one transition mutation compared with CRPV-KS, the CRPN genome that has been fully sequenced. Despite these differences, an intact E5 ORF is preserved, supporting the notion that this gene may serve a biological function. One frameshift and two in-frame mutations were constructed in the small region of the 5′ end of the E5 ORF that follows the E2 stop codon and precedes the L2 ORF. Several hundred rabbit skin sites were inoculated with each DNA preparation with a jet injector to test the ability of three CRPV E5 mutant DNAs to induce papillomas. In vivo results showed that each of the mutants induced papillomas, and biochemical analysis demonstrated that the E5 mutations present in DNA inocula were retained in the papillomas. The frequency of papilloma formation, however, was generally lower with each of the CRPV E5 mutants than with wiid-type CRPV DNA, particularly so for the E5 frameshift mutant, suggesting that although the recognized E5 ORF is not required in domestic rabbits for the induction of papillomas by CRPV DNA, it may facilitate their formation.

AB - In the cottontail rabbit papillomavirus (CRPV)-rabbit system, recombinant CRPV DNA can induce papillomas. This investigation was undertaken to evaluate whether the E5 open reading frame (ORF) of CRPV is required for papilloma formation. The CPRV genome we utilized, CRPV-WA, was sequenced in the E5 region and was found to contain one deletion, two insertions, and one transition mutation compared with CRPV-KS, the CRPN genome that has been fully sequenced. Despite these differences, an intact E5 ORF is preserved, supporting the notion that this gene may serve a biological function. One frameshift and two in-frame mutations were constructed in the small region of the 5′ end of the E5 ORF that follows the E2 stop codon and precedes the L2 ORF. Several hundred rabbit skin sites were inoculated with each DNA preparation with a jet injector to test the ability of three CRPV E5 mutant DNAs to induce papillomas. In vivo results showed that each of the mutants induced papillomas, and biochemical analysis demonstrated that the E5 mutations present in DNA inocula were retained in the papillomas. The frequency of papilloma formation, however, was generally lower with each of the CRPV E5 mutants than with wiid-type CRPV DNA, particularly so for the E5 frameshift mutant, suggesting that although the recognized E5 ORF is not required in domestic rabbits for the induction of papillomas by CRPV DNA, it may facilitate their formation.

UR - http://www.scopus.com/inward/record.url?scp=0026726881&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026726881&partnerID=8YFLogxK

M3 - Article

C2 - 1326666

AN - SCOPUS:0026726881

VL - 66

SP - 6204

EP - 6207

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 10

ER -