The proteins of Japanese encephalitis virus

Daniel Shapiro, Walter E. Brandt, Robert Cardiff, Philip K. Russell

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Polyacrylamide gel electrophoresis of Japanese encephalitis virus (JEV) grown in both LLC-MK2 and chick embryo cell culture revealed three principal polypeptides with molecular weights of 8,700, 13,500, and 53,000 (V-1, V-2, and V-3, respectively). Infected chick cells that were treated with actinomycin D and cycloheximide contained seven polypeptides not present in uninfected cells. In addition to V-2 and V-3, polypeptides with molecular weights of 10,500, 19,000, 45,000, 71,000, and 93,000 (NV-1 through NV-5) were found; V-1 was not regularly detected. A similar pattern of polypeptides was obtained by radioimmune precipitation of soluble antigens from cytoplasmic extracts of infected, actinomycin-D treated, chick cells. When virions were treated with NP-40, a dense, RNA-rich structure was detected which contained V-2. An extracellular, slowly sedimenting, RNA-poor, hemagglutinating particle with a density comparable to the virion was present in virus preparations from cell culture and contained V-1, V-3, and NV-2.

Original languageEnglish (US)
Pages (from-to)108-124
Number of pages17
JournalVirology
Volume44
Issue number1
DOIs
StatePublished - Jan 1 1971
Externally publishedYes

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Japanese Encephalitis Virus
Peptides
Dactinomycin
Virion
Proteins
Cell Culture Techniques
Molecular Weight
RNA
Chick Embryo
Cycloheximide
Polyacrylamide Gel Electrophoresis
Viruses
Antigens

ASJC Scopus subject areas

  • Virology

Cite this

Shapiro, D., Brandt, W. E., Cardiff, R., & Russell, P. K. (1971). The proteins of Japanese encephalitis virus. Virology, 44(1), 108-124. https://doi.org/10.1016/0042-6822(71)90158-9

The proteins of Japanese encephalitis virus. / Shapiro, Daniel; Brandt, Walter E.; Cardiff, Robert; Russell, Philip K.

In: Virology, Vol. 44, No. 1, 01.01.1971, p. 108-124.

Research output: Contribution to journalArticle

Shapiro, D, Brandt, WE, Cardiff, R & Russell, PK 1971, 'The proteins of Japanese encephalitis virus', Virology, vol. 44, no. 1, pp. 108-124. https://doi.org/10.1016/0042-6822(71)90158-9
Shapiro, Daniel ; Brandt, Walter E. ; Cardiff, Robert ; Russell, Philip K. / The proteins of Japanese encephalitis virus. In: Virology. 1971 ; Vol. 44, No. 1. pp. 108-124.
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