The promigratory activity of the matricellular protein galectin-3 depends on the activation of pi-3 kinase

Fabiana H M Melo, Diego Butera, Mara Souza de Junqueira, Daniel K. Hsu, Ana Maria Moura da Silva, Fu-Tong Liu, Marinilice F. Santos, Roger Chammas

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Expression of galectin-3 is associated with sarcoma progression, invasion and metastasis. Here we determined the role of extracellular galectin-3 on migration of sarcoma cells on laminin-111. Cell lines from methylcholanthrene-induced sarcomas from both wild type and galectin-3 -/- mice were established. Despite the presence of similar levels of laminin-binding integrins on the cell surface, galectin-3 -/- sarcoma cells were more adherent and less migratory than galectin-3 +/+ sarcoma cells on laminin-111. When galectin-3 was transiently expressed in galectin-3 -/- sarcoma cells, it inhibited cell adhesion and stimulated the migratory response to laminin in a carbohydrate-dependent manner. Extracellular galectin-3 led to the recruitment of SHP-2 phosphatase to focal adhesion plaques, followed by a decrease in the amount of phosphorylated FAK and phospho-paxillin in the lamellipodia of migrating cells. The promigratory activity of extracellular galectin-3 was inhibitable by wortmannin, implicating the activation of a PI-3 kinase dependent pathway in the galectin-3 triggered disruption of adhesion plaques, leading to sarcoma cell migration on laminin-111.

Original languageEnglish (US)
Article numbere29313
JournalPLoS One
Volume6
Issue number12
DOIs
StatePublished - Dec 28 2011

Fingerprint

Galectin 3
sarcoma
laminin
phosphotransferases (kinases)
Phosphotransferases
Chemical activation
Sarcoma
Laminin
Proteins
proteins
Focal Adhesions
cells
adhesion
pseudopodia
Cell Movement
integrins
phosphatidylinositol 3-kinase
Adhesion
cell movement
cell adhesion

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Melo, F. H. M., Butera, D., de Junqueira, M. S., Hsu, D. K., da Silva, A. M. M., Liu, F-T., ... Chammas, R. (2011). The promigratory activity of the matricellular protein galectin-3 depends on the activation of pi-3 kinase. PLoS One, 6(12), [e29313]. https://doi.org/10.1371/journal.pone.0029313

The promigratory activity of the matricellular protein galectin-3 depends on the activation of pi-3 kinase. / Melo, Fabiana H M; Butera, Diego; de Junqueira, Mara Souza; Hsu, Daniel K.; da Silva, Ana Maria Moura; Liu, Fu-Tong; Santos, Marinilice F.; Chammas, Roger.

In: PLoS One, Vol. 6, No. 12, e29313, 28.12.2011.

Research output: Contribution to journalArticle

Melo, FHM, Butera, D, de Junqueira, MS, Hsu, DK, da Silva, AMM, Liu, F-T, Santos, MF & Chammas, R 2011, 'The promigratory activity of the matricellular protein galectin-3 depends on the activation of pi-3 kinase', PLoS One, vol. 6, no. 12, e29313. https://doi.org/10.1371/journal.pone.0029313
Melo, Fabiana H M ; Butera, Diego ; de Junqueira, Mara Souza ; Hsu, Daniel K. ; da Silva, Ana Maria Moura ; Liu, Fu-Tong ; Santos, Marinilice F. ; Chammas, Roger. / The promigratory activity of the matricellular protein galectin-3 depends on the activation of pi-3 kinase. In: PLoS One. 2011 ; Vol. 6, No. 12.
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