The product of the avian erythroblastosis virus erbB locus is a glycoprotein

Martin L. Privalsky; Linda Sealy; J. Michael Bishop; John P. McGrath; Arthur D. Levinson

doi:10.1016/0092-8674(83)90307-0

The product of the avian erythroblastosis virus erbB locus is a glycoprotein

Martin L. Privalsky, Linda Sealy, J. Michael Bishop, John P. McGrath, Arthur D. Levinson

Microbiology

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57 Scopus citations

Abstract

Avian erythroblastosis virus (AEV) induces both erythroblastosis and fibrosarcomas in susceptible birds. A locus, v-erbB, within the viral genome has been implicated in AEV-mediated oncogenesis. We report here the detection and partial characterization of the protein product of the v-erbB oncogene in AEV-transformed cells. We obtained the antisera necessary for our analysis by expressing a portion of the molecularly cloned v-erbB locus in Escherichia coli and immunizing rabbits with the resulting bacterial erbB polypeptide. Antisera directed against the bacterial polypeptide reacted with v-erbB proteins obtained from virus-infected avian cells. By three criteria-tunicamycin inhibition, lectin binding and metabolic labeling with radioactive sugar precursors-the product of the v-erbB gene appears to be a glycoprotein.

Original language	English (US)
Pages (from-to)	1257-1267
Number of pages	11
Journal	Cell
Volume	32
Issue number	4
DOIs	https://doi.org/10.1016/0092-8674(83)90307-0
State	Published - 1983

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ASJC Scopus subject areas

Cell Biology
Molecular Biology

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Privalsky, M. L., Sealy, L., Michael Bishop, J., McGrath, J. P., & Levinson, A. D. (1983). The product of the avian erythroblastosis virus erbB locus is a glycoprotein. Cell, 32(4), 1257-1267. https://doi.org/10.1016/0092-8674(83)90307-0

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abstract = "Avian erythroblastosis virus (AEV) induces both erythroblastosis and fibrosarcomas in susceptible birds. A locus, v-erbB, within the viral genome has been implicated in AEV-mediated oncogenesis. We report here the detection and partial characterization of the protein product of the v-erbB oncogene in AEV-transformed cells. We obtained the antisera necessary for our analysis by expressing a portion of the molecularly cloned v-erbB locus in Escherichia coli and immunizing rabbits with the resulting bacterial erbB polypeptide. Antisera directed against the bacterial polypeptide reacted with v-erbB proteins obtained from virus-infected avian cells. By three criteria-tunicamycin inhibition, lectin binding and metabolic labeling with radioactive sugar precursors-the product of the v-erbB gene appears to be a glycoprotein.",

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AB - Avian erythroblastosis virus (AEV) induces both erythroblastosis and fibrosarcomas in susceptible birds. A locus, v-erbB, within the viral genome has been implicated in AEV-mediated oncogenesis. We report here the detection and partial characterization of the protein product of the v-erbB oncogene in AEV-transformed cells. We obtained the antisera necessary for our analysis by expressing a portion of the molecularly cloned v-erbB locus in Escherichia coli and immunizing rabbits with the resulting bacterial erbB polypeptide. Antisera directed against the bacterial polypeptide reacted with v-erbB proteins obtained from virus-infected avian cells. By three criteria-tunicamycin inhibition, lectin binding and metabolic labeling with radioactive sugar precursors-the product of the v-erbB gene appears to be a glycoprotein.

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10.1016/0092-8674(83)90307-0