Abstract
Avian erythroblastosis virus (AEV) induces both erythroblastosis and fibrosarcomas in susceptible birds. A locus, v-erbB, within the viral genome has been implicated in AEV-mediated oncogenesis. We report here the detection and partial characterization of the protein product of the v-erbB oncogene in AEV-transformed cells. We obtained the antisera necessary for our analysis by expressing a portion of the molecularly cloned v-erbB locus in Escherichia coli and immunizing rabbits with the resulting bacterial erbB polypeptide. Antisera directed against the bacterial polypeptide reacted with v-erbB proteins obtained from virus-infected avian cells. By three criteria-tunicamycin inhibition, lectin binding and metabolic labeling with radioactive sugar precursors-the product of the v-erbB gene appears to be a glycoprotein.
Original language | English (US) |
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Pages (from-to) | 1257-1267 |
Number of pages | 11 |
Journal | Cell |
Volume | 32 |
Issue number | 4 |
DOIs | |
State | Published - 1983 |
ASJC Scopus subject areas
- Cell Biology
- Molecular Biology