The product of the avian erythroblastosis virus erbB locus is a glycoprotein

Martin L. Privalsky, Linda Sealy, J. Michael Bishop, John P. McGrath, Arthur D. Levinson

Research output: Contribution to journalArticle

57 Scopus citations

Abstract

Avian erythroblastosis virus (AEV) induces both erythroblastosis and fibrosarcomas in susceptible birds. A locus, v-erbB, within the viral genome has been implicated in AEV-mediated oncogenesis. We report here the detection and partial characterization of the protein product of the v-erbB oncogene in AEV-transformed cells. We obtained the antisera necessary for our analysis by expressing a portion of the molecularly cloned v-erbB locus in Escherichia coli and immunizing rabbits with the resulting bacterial erbB polypeptide. Antisera directed against the bacterial polypeptide reacted with v-erbB proteins obtained from virus-infected avian cells. By three criteria-tunicamycin inhibition, lectin binding and metabolic labeling with radioactive sugar precursors-the product of the v-erbB gene appears to be a glycoprotein.

Original languageEnglish (US)
Pages (from-to)1257-1267
Number of pages11
JournalCell
Volume32
Issue number4
DOIs
StatePublished - 1983

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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    Privalsky, M. L., Sealy, L., Michael Bishop, J., McGrath, J. P., & Levinson, A. D. (1983). The product of the avian erythroblastosis virus erbB locus is a glycoprotein. Cell, 32(4), 1257-1267. https://doi.org/10.1016/0092-8674(83)90307-0