The polar T1 interface is linked to conformational changes that open the voltage-gated potassium channel

Daniel L. Minor, Yu-Fung Lin, Bret C. Mobley, Abigail Avelar, Yuh Nung Jan, Lily Y. Jan, James M. Berger

Research output: Contribution to journalArticle

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Abstract

Kv voltage-gated potassium channels share a cytoplasmic assembly domain, T1. Recent mutagenesis of two T1 C-terminal loop residues implicates T1 in channel gating. However, structural alterations of these mutants leave open the question concerning direct involvement of T1 in gating. We find in mammalian Kv1.2 that gating depends critically on residues at complementary T1 surfaces in an unusually polar interface. An isosteric mutation in this interface causes surprisingly little structural alteration while stabilizing the closed channel and increasing the stability of T1 tetramers. Replacing T1 with a tetrameric coiled-coil destabilizes the closed channel. Together, these data suggest that structural changes involving the buried polar T1 surfaces play a key role in the conformational changes leading to channel opening.

Original languageEnglish (US)
Pages (from-to)657-670
Number of pages14
JournalCell
Volume102
Issue number5
StatePublished - Sep 1 2000

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Voltage-Gated Potassium Channels
Mutagenesis
Mutation

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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Minor, D. L., Lin, Y-F., Mobley, B. C., Avelar, A., Jan, Y. N., Jan, L. Y., & Berger, J. M. (2000). The polar T1 interface is linked to conformational changes that open the voltage-gated potassium channel. Cell, 102(5), 657-670.

The polar T1 interface is linked to conformational changes that open the voltage-gated potassium channel. / Minor, Daniel L.; Lin, Yu-Fung; Mobley, Bret C.; Avelar, Abigail; Jan, Yuh Nung; Jan, Lily Y.; Berger, James M.

In: Cell, Vol. 102, No. 5, 01.09.2000, p. 657-670.

Research output: Contribution to journalArticle

Minor, DL, Lin, Y-F, Mobley, BC, Avelar, A, Jan, YN, Jan, LY & Berger, JM 2000, 'The polar T1 interface is linked to conformational changes that open the voltage-gated potassium channel', Cell, vol. 102, no. 5, pp. 657-670.
Minor DL, Lin Y-F, Mobley BC, Avelar A, Jan YN, Jan LY et al. The polar T1 interface is linked to conformational changes that open the voltage-gated potassium channel. Cell. 2000 Sep 1;102(5):657-670.
Minor, Daniel L. ; Lin, Yu-Fung ; Mobley, Bret C. ; Avelar, Abigail ; Jan, Yuh Nung ; Jan, Lily Y. ; Berger, James M. / The polar T1 interface is linked to conformational changes that open the voltage-gated potassium channel. In: Cell. 2000 ; Vol. 102, No. 5. pp. 657-670.
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