The plasma membrane plays a central role in cells response to mechanical stress

Sandra V. Verstraeten, Gerardo Mackenzie, Patricia I. Oteiza

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The mechanisms by which lymphocytes recognize and interpret mechanical stimuli and translate these into the triggering of select signaling cascades that are critical for lymphocyte function are still not fully understood. In this work, we investigated the association of mechanical stress (MS)-induced changes in membrane physical properties with changes in cytoskeleton dynamics and cell signaling. In Jurkat T cells, MS was associated with the immediate and transient depolymerization of both β-tubulin and F-actin. The fluidity of the plasma membrane measured in the hydrophobic region of the bilayer, increased 0.5min post-MS, recovering the initial value in the following 2min. This effect was accompanied by the rearrangement of lipids in the lateral phase of the plasma membrane, transient lipid rafts' alteration, and membrane hyperpolarization. The consequent increase in cellular [Ca2+] triggered the activation of the transcription factors NFAT, AP-1, and NF-κB. Results indicate that the cytoplasmic membrane, through changes in membrane physical properties, senses MS, and transduces an initial physical stimulus into microtubules rearrangements, Ca2+ mobilization, and the subsequent changes in cell signaling.

Original languageEnglish (US)
Pages (from-to)1739-1749
Number of pages11
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1798
Issue number9
DOIs
StatePublished - Sep 2010

Fingerprint

Mechanical Stress
Cell membranes
Cell Membrane
Cell signaling
Membranes
Lymphocytes
Physical properties
Lipids
Depolymerization
Jurkat Cells
T-cells
Fluidity
Transcription Factor AP-1
Tubulin
Membrane Lipids
Cytoskeleton
Microtubules
Actins
Transcription Factors
Chemical activation

Keywords

  • Calcium
  • Lipid lateral mobility
  • Mechanical stress
  • Membrane fluidity
  • Signal transduction

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biophysics
  • Medicine(all)

Cite this

The plasma membrane plays a central role in cells response to mechanical stress. / Verstraeten, Sandra V.; Mackenzie, Gerardo; Oteiza, Patricia I.

In: Biochimica et Biophysica Acta - Biomembranes, Vol. 1798, No. 9, 09.2010, p. 1739-1749.

Research output: Contribution to journalArticle

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