Abstract
The mechanisms by which lymphocytes recognize and interpret mechanical stimuli and translate these into the triggering of select signaling cascades that are critical for lymphocyte function are still not fully understood. In this work, we investigated the association of mechanical stress (MS)-induced changes in membrane physical properties with changes in cytoskeleton dynamics and cell signaling. In Jurkat T cells, MS was associated with the immediate and transient depolymerization of both β-tubulin and F-actin. The fluidity of the plasma membrane measured in the hydrophobic region of the bilayer, increased 0.5min post-MS, recovering the initial value in the following 2min. This effect was accompanied by the rearrangement of lipids in the lateral phase of the plasma membrane, transient lipid rafts' alteration, and membrane hyperpolarization. The consequent increase in cellular [Ca2+] triggered the activation of the transcription factors NFAT, AP-1, and NF-κB. Results indicate that the cytoplasmic membrane, through changes in membrane physical properties, senses MS, and transduces an initial physical stimulus into microtubules rearrangements, Ca2+ mobilization, and the subsequent changes in cell signaling.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1739-1749 |
| Number of pages | 11 |
| Journal | Biochimica et Biophysica Acta - Biomembranes |
| Volume | 1798 |
| Issue number | 9 |
| DOIs | |
| State | Published - Sep 2010 |
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Keywords
- Calcium
- Lipid lateral mobility
- Mechanical stress
- Membrane fluidity
- Signal transduction
ASJC Scopus subject areas
- Biochemistry
- Cell Biology
- Biophysics
- Medicine(all)
Cite this
The plasma membrane plays a central role in cells response to mechanical stress. / Verstraeten, Sandra V.; Mackenzie, Gerardo; Oteiza, Patricia I.
In: Biochimica et Biophysica Acta - Biomembranes, Vol. 1798, No. 9, 09.2010, p. 1739-1749.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The plasma membrane plays a central role in cells response to mechanical stress
AU - Verstraeten, Sandra V.
AU - Mackenzie, Gerardo
AU - Oteiza, Patricia I.
PY - 2010/9
Y1 - 2010/9
N2 - The mechanisms by which lymphocytes recognize and interpret mechanical stimuli and translate these into the triggering of select signaling cascades that are critical for lymphocyte function are still not fully understood. In this work, we investigated the association of mechanical stress (MS)-induced changes in membrane physical properties with changes in cytoskeleton dynamics and cell signaling. In Jurkat T cells, MS was associated with the immediate and transient depolymerization of both β-tubulin and F-actin. The fluidity of the plasma membrane measured in the hydrophobic region of the bilayer, increased 0.5min post-MS, recovering the initial value in the following 2min. This effect was accompanied by the rearrangement of lipids in the lateral phase of the plasma membrane, transient lipid rafts' alteration, and membrane hyperpolarization. The consequent increase in cellular [Ca2+] triggered the activation of the transcription factors NFAT, AP-1, and NF-κB. Results indicate that the cytoplasmic membrane, through changes in membrane physical properties, senses MS, and transduces an initial physical stimulus into microtubules rearrangements, Ca2+ mobilization, and the subsequent changes in cell signaling.
AB - The mechanisms by which lymphocytes recognize and interpret mechanical stimuli and translate these into the triggering of select signaling cascades that are critical for lymphocyte function are still not fully understood. In this work, we investigated the association of mechanical stress (MS)-induced changes in membrane physical properties with changes in cytoskeleton dynamics and cell signaling. In Jurkat T cells, MS was associated with the immediate and transient depolymerization of both β-tubulin and F-actin. The fluidity of the plasma membrane measured in the hydrophobic region of the bilayer, increased 0.5min post-MS, recovering the initial value in the following 2min. This effect was accompanied by the rearrangement of lipids in the lateral phase of the plasma membrane, transient lipid rafts' alteration, and membrane hyperpolarization. The consequent increase in cellular [Ca2+] triggered the activation of the transcription factors NFAT, AP-1, and NF-κB. Results indicate that the cytoplasmic membrane, through changes in membrane physical properties, senses MS, and transduces an initial physical stimulus into microtubules rearrangements, Ca2+ mobilization, and the subsequent changes in cell signaling.
KW - Calcium
KW - Lipid lateral mobility
KW - Mechanical stress
KW - Membrane fluidity
KW - Signal transduction
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UR - http://www.scopus.com/inward/citedby.url?scp=77954657439&partnerID=8YFLogxK
U2 - 10.1016/j.bbamem.2010.06.010
DO - 10.1016/j.bbamem.2010.06.010
M3 - Article
C2 - 20599684
AN - SCOPUS:77954657439
VL - 1798
SP - 1739
EP - 1749
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
SN - 0005-2736
IS - 9
ER -