The PIE-1 protein and germline specification in C. elegans embryos

C. C. Mello, C. Schubert, B. Draper, W. Zhang, R. Lobel, J. R. Priess

Research output: Contribution to journalArticle

248 Citations (Scopus)

Abstract

Totipotent germline blastomeres in Caenorhabditis elegans contain, but do not respond to, factors that promote somatic differentiation in other embryonic cells. Mutations in the maternal gene pie-I result in the germline blastomeres adopting somatic cell fates. Here we show that pie-1 encodes a nuclear protein, PIE-I, that is localized to the germline blastomeres throughout early development. During division of each germline blastomere, PIE-1 initially associates with both centrosomes of the mitotic spindle. However, PIE-1 rapidly disappears from the centrosome destined for the somatic daughter, and persists in the centrosome of the daughter that becomes the next germline blastomere. The PIE-1 protein contains potential zinc- finger motifs also found in the mammalian growth-factor response protein TIS- 11/NUP475. The localization and genetic properties pie-1 provide an example of a repressor-based mechanism for preserving pluripotency within a stem cell lineage.

Original languageEnglish (US)
Pages (from-to)710-712
Number of pages3
JournalNature
Volume382
Issue number6593
DOIs
StatePublished - 1996
Externally publishedYes

Fingerprint

Blastomeres
Embryonic Structures
Centrosome
Proteins
Spindle Apparatus
Zinc Fingers
Caenorhabditis elegans
Cell Lineage
Nuclear Proteins
Intercellular Signaling Peptides and Proteins
Stem Cells
Mothers
Mutation
Genes

ASJC Scopus subject areas

  • General

Cite this

Mello, C. C., Schubert, C., Draper, B., Zhang, W., Lobel, R., & Priess, J. R. (1996). The PIE-1 protein and germline specification in C. elegans embryos. Nature, 382(6593), 710-712. https://doi.org/10.1038/382710a0

The PIE-1 protein and germline specification in C. elegans embryos. / Mello, C. C.; Schubert, C.; Draper, B.; Zhang, W.; Lobel, R.; Priess, J. R.

In: Nature, Vol. 382, No. 6593, 1996, p. 710-712.

Research output: Contribution to journalArticle

Mello, CC, Schubert, C, Draper, B, Zhang, W, Lobel, R & Priess, JR 1996, 'The PIE-1 protein and germline specification in C. elegans embryos', Nature, vol. 382, no. 6593, pp. 710-712. https://doi.org/10.1038/382710a0
Mello CC, Schubert C, Draper B, Zhang W, Lobel R, Priess JR. The PIE-1 protein and germline specification in C. elegans embryos. Nature. 1996;382(6593):710-712. https://doi.org/10.1038/382710a0
Mello, C. C. ; Schubert, C. ; Draper, B. ; Zhang, W. ; Lobel, R. ; Priess, J. R. / The PIE-1 protein and germline specification in C. elegans embryos. In: Nature. 1996 ; Vol. 382, No. 6593. pp. 710-712.
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