The PIAS-like protein Zimp10 is essential for embryonic viability and proper vascular development

Jason Beliakoff, Jane Lee, Hiroo Ueno, Aparna Aiyer, Irving L. Weissman, Gregory S. Barsh, Robert Cardiff, Zijie Sun

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Members of the PIAS (for protein inhibitor of activated STAT) family play critical roles in modulating the activity of a variety of transcriptional regulators. Zimp10, a novel PIAS-like protein, is a transcriptional coregulator and may be involved in the modification of chromatin through interactions with the SWI/SNF chromatin-remodeling complexes. Here, we investigate the biological role of Zimp10 in zimp10-deficient mice. Homozygosity for the Zimp10-targeted allele resulted in developmental arrest at approximately embryonic day 10.5. Analysis of knockout embryos revealed severe defects in the reorganization of the yolk sac vascular plexus. No significant abnormality in hematopoietic potential was observed in zimp10 null mice. Microarray and quantified reverse transcription-PCR analyses showed that the expression of the Fos family member Fra-1, which is involved in extraembryonic vascular development, was reduced in yolk sac tissues of zimp10 null embryos. Using fra-1 promoter/reporter constructs, we further demonstrate the regulatory role of Zimp10 on the transcription of Fra-1. This study provides evidence to demonstrate a crucial role for Zimp10 in vasculogenesis.

Original languageEnglish (US)
Pages (from-to)282-292
Number of pages11
JournalMolecular and Cellular Biology
Volume28
Issue number1
DOIs
StatePublished - Jan 1 2008

Fingerprint

Protein Inhibitors of Activated STAT
Yolk Sac
Blood Vessels
Embryonic Structures
Chromatin Assembly and Disassembly
Reverse Transcription
Chromatin
Proteins
Alleles
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Beliakoff, J., Lee, J., Ueno, H., Aiyer, A., Weissman, I. L., Barsh, G. S., ... Sun, Z. (2008). The PIAS-like protein Zimp10 is essential for embryonic viability and proper vascular development. Molecular and Cellular Biology, 28(1), 282-292. https://doi.org/10.1128/MCB.00771-07

The PIAS-like protein Zimp10 is essential for embryonic viability and proper vascular development. / Beliakoff, Jason; Lee, Jane; Ueno, Hiroo; Aiyer, Aparna; Weissman, Irving L.; Barsh, Gregory S.; Cardiff, Robert; Sun, Zijie.

In: Molecular and Cellular Biology, Vol. 28, No. 1, 01.01.2008, p. 282-292.

Research output: Contribution to journalArticle

Beliakoff, Jason ; Lee, Jane ; Ueno, Hiroo ; Aiyer, Aparna ; Weissman, Irving L. ; Barsh, Gregory S. ; Cardiff, Robert ; Sun, Zijie. / The PIAS-like protein Zimp10 is essential for embryonic viability and proper vascular development. In: Molecular and Cellular Biology. 2008 ; Vol. 28, No. 1. pp. 282-292.
@article{6718b4d8bd40467ab49985e41ee96e0e,
title = "The PIAS-like protein Zimp10 is essential for embryonic viability and proper vascular development",
abstract = "Members of the PIAS (for protein inhibitor of activated STAT) family play critical roles in modulating the activity of a variety of transcriptional regulators. Zimp10, a novel PIAS-like protein, is a transcriptional coregulator and may be involved in the modification of chromatin through interactions with the SWI/SNF chromatin-remodeling complexes. Here, we investigate the biological role of Zimp10 in zimp10-deficient mice. Homozygosity for the Zimp10-targeted allele resulted in developmental arrest at approximately embryonic day 10.5. Analysis of knockout embryos revealed severe defects in the reorganization of the yolk sac vascular plexus. No significant abnormality in hematopoietic potential was observed in zimp10 null mice. Microarray and quantified reverse transcription-PCR analyses showed that the expression of the Fos family member Fra-1, which is involved in extraembryonic vascular development, was reduced in yolk sac tissues of zimp10 null embryos. Using fra-1 promoter/reporter constructs, we further demonstrate the regulatory role of Zimp10 on the transcription of Fra-1. This study provides evidence to demonstrate a crucial role for Zimp10 in vasculogenesis.",
author = "Jason Beliakoff and Jane Lee and Hiroo Ueno and Aparna Aiyer and Weissman, {Irving L.} and Barsh, {Gregory S.} and Robert Cardiff and Zijie Sun",
year = "2008",
month = "1",
day = "1",
doi = "10.1128/MCB.00771-07",
language = "English (US)",
volume = "28",
pages = "282--292",
journal = "Molecular and Cellular Biology",
issn = "0270-7306",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - The PIAS-like protein Zimp10 is essential for embryonic viability and proper vascular development

AU - Beliakoff, Jason

AU - Lee, Jane

AU - Ueno, Hiroo

AU - Aiyer, Aparna

AU - Weissman, Irving L.

AU - Barsh, Gregory S.

AU - Cardiff, Robert

AU - Sun, Zijie

PY - 2008/1/1

Y1 - 2008/1/1

N2 - Members of the PIAS (for protein inhibitor of activated STAT) family play critical roles in modulating the activity of a variety of transcriptional regulators. Zimp10, a novel PIAS-like protein, is a transcriptional coregulator and may be involved in the modification of chromatin through interactions with the SWI/SNF chromatin-remodeling complexes. Here, we investigate the biological role of Zimp10 in zimp10-deficient mice. Homozygosity for the Zimp10-targeted allele resulted in developmental arrest at approximately embryonic day 10.5. Analysis of knockout embryos revealed severe defects in the reorganization of the yolk sac vascular plexus. No significant abnormality in hematopoietic potential was observed in zimp10 null mice. Microarray and quantified reverse transcription-PCR analyses showed that the expression of the Fos family member Fra-1, which is involved in extraembryonic vascular development, was reduced in yolk sac tissues of zimp10 null embryos. Using fra-1 promoter/reporter constructs, we further demonstrate the regulatory role of Zimp10 on the transcription of Fra-1. This study provides evidence to demonstrate a crucial role for Zimp10 in vasculogenesis.

AB - Members of the PIAS (for protein inhibitor of activated STAT) family play critical roles in modulating the activity of a variety of transcriptional regulators. Zimp10, a novel PIAS-like protein, is a transcriptional coregulator and may be involved in the modification of chromatin through interactions with the SWI/SNF chromatin-remodeling complexes. Here, we investigate the biological role of Zimp10 in zimp10-deficient mice. Homozygosity for the Zimp10-targeted allele resulted in developmental arrest at approximately embryonic day 10.5. Analysis of knockout embryos revealed severe defects in the reorganization of the yolk sac vascular plexus. No significant abnormality in hematopoietic potential was observed in zimp10 null mice. Microarray and quantified reverse transcription-PCR analyses showed that the expression of the Fos family member Fra-1, which is involved in extraembryonic vascular development, was reduced in yolk sac tissues of zimp10 null embryos. Using fra-1 promoter/reporter constructs, we further demonstrate the regulatory role of Zimp10 on the transcription of Fra-1. This study provides evidence to demonstrate a crucial role for Zimp10 in vasculogenesis.

UR - http://www.scopus.com/inward/record.url?scp=37549060391&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=37549060391&partnerID=8YFLogxK

U2 - 10.1128/MCB.00771-07

DO - 10.1128/MCB.00771-07

M3 - Article

C2 - 17967885

AN - SCOPUS:37549060391

VL - 28

SP - 282

EP - 292

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

SN - 0270-7306

IS - 1

ER -