Abstract
Members of the PIAS (for protein inhibitor of activated STAT) family play critical roles in modulating the activity of a variety of transcriptional regulators. Zimp10, a novel PIAS-like protein, is a transcriptional coregulator and may be involved in the modification of chromatin through interactions with the SWI/SNF chromatin-remodeling complexes. Here, we investigate the biological role of Zimp10 in zimp10-deficient mice. Homozygosity for the Zimp10-targeted allele resulted in developmental arrest at approximately embryonic day 10.5. Analysis of knockout embryos revealed severe defects in the reorganization of the yolk sac vascular plexus. No significant abnormality in hematopoietic potential was observed in zimp10 null mice. Microarray and quantified reverse transcription-PCR analyses showed that the expression of the Fos family member Fra-1, which is involved in extraembryonic vascular development, was reduced in yolk sac tissues of zimp10 null embryos. Using fra-1 promoter/reporter constructs, we further demonstrate the regulatory role of Zimp10 on the transcription of Fra-1. This study provides evidence to demonstrate a crucial role for Zimp10 in vasculogenesis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 282-292 |
| Number of pages | 11 |
| Journal | Molecular and Cellular Biology |
| Volume | 28 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 2008 |
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ASJC Scopus subject areas
- Molecular Biology
- Cell Biology
Cite this
The PIAS-like protein Zimp10 is essential for embryonic viability and proper vascular development. / Beliakoff, Jason; Lee, Jane; Ueno, Hiroo; Aiyer, Aparna; Weissman, Irving L.; Barsh, Gregory S.; Cardiff, Robert; Sun, Zijie.
In: Molecular and Cellular Biology, Vol. 28, No. 1, 01.01.2008, p. 282-292.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The PIAS-like protein Zimp10 is essential for embryonic viability and proper vascular development
AU - Beliakoff, Jason
AU - Lee, Jane
AU - Ueno, Hiroo
AU - Aiyer, Aparna
AU - Weissman, Irving L.
AU - Barsh, Gregory S.
AU - Cardiff, Robert
AU - Sun, Zijie
PY - 2008/1/1
Y1 - 2008/1/1
N2 - Members of the PIAS (for protein inhibitor of activated STAT) family play critical roles in modulating the activity of a variety of transcriptional regulators. Zimp10, a novel PIAS-like protein, is a transcriptional coregulator and may be involved in the modification of chromatin through interactions with the SWI/SNF chromatin-remodeling complexes. Here, we investigate the biological role of Zimp10 in zimp10-deficient mice. Homozygosity for the Zimp10-targeted allele resulted in developmental arrest at approximately embryonic day 10.5. Analysis of knockout embryos revealed severe defects in the reorganization of the yolk sac vascular plexus. No significant abnormality in hematopoietic potential was observed in zimp10 null mice. Microarray and quantified reverse transcription-PCR analyses showed that the expression of the Fos family member Fra-1, which is involved in extraembryonic vascular development, was reduced in yolk sac tissues of zimp10 null embryos. Using fra-1 promoter/reporter constructs, we further demonstrate the regulatory role of Zimp10 on the transcription of Fra-1. This study provides evidence to demonstrate a crucial role for Zimp10 in vasculogenesis.
AB - Members of the PIAS (for protein inhibitor of activated STAT) family play critical roles in modulating the activity of a variety of transcriptional regulators. Zimp10, a novel PIAS-like protein, is a transcriptional coregulator and may be involved in the modification of chromatin through interactions with the SWI/SNF chromatin-remodeling complexes. Here, we investigate the biological role of Zimp10 in zimp10-deficient mice. Homozygosity for the Zimp10-targeted allele resulted in developmental arrest at approximately embryonic day 10.5. Analysis of knockout embryos revealed severe defects in the reorganization of the yolk sac vascular plexus. No significant abnormality in hematopoietic potential was observed in zimp10 null mice. Microarray and quantified reverse transcription-PCR analyses showed that the expression of the Fos family member Fra-1, which is involved in extraembryonic vascular development, was reduced in yolk sac tissues of zimp10 null embryos. Using fra-1 promoter/reporter constructs, we further demonstrate the regulatory role of Zimp10 on the transcription of Fra-1. This study provides evidence to demonstrate a crucial role for Zimp10 in vasculogenesis.
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UR - http://www.scopus.com/inward/citedby.url?scp=37549060391&partnerID=8YFLogxK
U2 - 10.1128/MCB.00771-07
DO - 10.1128/MCB.00771-07
M3 - Article
C2 - 17967885
AN - SCOPUS:37549060391
VL - 28
SP - 282
EP - 292
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
SN - 0270-7306
IS - 1
ER -