The phylogenetic relationships of Chlorobium tepidum and Chloroflexus aurantiacus based upon their RecA sequences

Tanja M. Gruber, Jonathan A Eisen, Kurt Gish, Donald A. Bryant

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Using RecA as the phylogenetic marker, the relationships of the green sulfur bacterium Chlorobium tepidum and the green non-sulfur bacterium Chloroflexus aurantiacus to other eubacteria were investigated. The recA genes of the two organisms were cloned, and the resulting protein sequences aligned with 86 other eubacterial RecA sequences. Cb. tepidum was placed as the nearest relative to the Cytophaga/Flexibacter/Bacteroides group, a relationship supported by results obtained with several phylogenetic markers. Cf aurantiacus was placed near Chlamydia trachomatis and the high-GC Gram- positives; however, this branching pattern was not strongly supported statistically by bootstrap analyses. Possible reasons for this ambiguity are discussed.

Original languageEnglish (US)
Pages (from-to)53-60
Number of pages8
JournalFEMS Microbiology Letters
Volume162
Issue number1
DOIs
StatePublished - May 1 1998
Externally publishedYes

Fingerprint

Flexibacter
Chlorobium
Chlorobi
Chloroflexi
Cytophaga
Bacteroides
Chlamydia trachomatis
Bacteria
Genes
Proteins
Chloroflexus

Keywords

  • Chlorobium tepidum
  • Chloroflexus aurantiacus
  • Green bacterium
  • Molecular systematics
  • Phylogenetics
  • recA

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

The phylogenetic relationships of Chlorobium tepidum and Chloroflexus aurantiacus based upon their RecA sequences. / Gruber, Tanja M.; Eisen, Jonathan A; Gish, Kurt; Bryant, Donald A.

In: FEMS Microbiology Letters, Vol. 162, No. 1, 01.05.1998, p. 53-60.

Research output: Contribution to journalArticle

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