The phosphorylation state of an autoregulatory domain controls PACS-1-directed protein traffic

Gregory K. Scott, Feng Gu, Colin M. Crump, Laurel Thomas, Lei Wan, Yang Kevin Xiang, Gary Thomas

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

PACS-1 is a cytosolic sorting protein that directs the localization of membrane proteins in the trans-Golgi network (TGN)/endosomal system. PACS-1 connects the clathrin adaptor AP-1 to acidic cluster sorting motifs contained in the cytoplasmic domain of cargo proteins such as furin, the cation-independent mannose-6-phosphate receptor and in viral proteins such as human immunodeficiency virus type 1 Nef. Here we show that an acidic cluster on PACS-1, which is highly similar to acidic cluster sorting motifs on cargo molecules, acts as an autoregulatory domain that controls PACS-1-directed sorting. Biochemical studies show that Ser278 adjacent to the acidic cluster is phosphorylated by CK2 and dephosphorylated by PP2A. Phosphorylation of Ser278 by CK2 or a Ser278→Asp mutation increased the interaction between PACS-1 and cargo, whereas a Ser278→Ala substitution decreased this interaction. Moreover, the Ser278→Ala mutation yields a dominant-negative PACS-1 molecule that selectively blocks retrieval of PACS-1-regulated cargo molecules to the TGN. These results suggest that coordinated signaling events regulate transport within the TGN/endosomal system through the phosphorylation state of both cargo and the sorting machinery.

Original languageEnglish (US)
Pages (from-to)6234-6244
Number of pages11
JournalEMBO Journal
Volume22
Issue number23
DOIs
StatePublished - Dec 1 2003
Externally publishedYes

Fingerprint

trans-Golgi Network
Picture archiving and communication systems
Phosphorylation
Sorting
Vesicular Transport Adaptor Proteins
Furin
IGF Type 2 Receptor
Mutation
Proteins
Transcription Factor AP-1
Viral Proteins
Protein Transport
Cations
HIV-1
Membrane Proteins
Molecules
Viruses
Machinery
Substitution reactions

Keywords

  • Autoregulatory domain
  • CK2
  • Nef
  • PACS-1
  • Phosphorylation

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

The phosphorylation state of an autoregulatory domain controls PACS-1-directed protein traffic. / Scott, Gregory K.; Gu, Feng; Crump, Colin M.; Thomas, Laurel; Wan, Lei; Xiang, Yang Kevin; Thomas, Gary.

In: EMBO Journal, Vol. 22, No. 23, 01.12.2003, p. 6234-6244.

Research output: Contribution to journalArticle

Scott, GK, Gu, F, Crump, CM, Thomas, L, Wan, L, Xiang, YK & Thomas, G 2003, 'The phosphorylation state of an autoregulatory domain controls PACS-1-directed protein traffic', EMBO Journal, vol. 22, no. 23, pp. 6234-6244. https://doi.org/10.1093/emboj/cdg596
Scott, Gregory K. ; Gu, Feng ; Crump, Colin M. ; Thomas, Laurel ; Wan, Lei ; Xiang, Yang Kevin ; Thomas, Gary. / The phosphorylation state of an autoregulatory domain controls PACS-1-directed protein traffic. In: EMBO Journal. 2003 ; Vol. 22, No. 23. pp. 6234-6244.
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