The pathogenesis of experimental bluetongue virus infection of calves.

Nigel J Maclachlan, G. Jagels, P. V. Rossitto, Peter F Moore, H. W. Heidner

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Abstract

Eleven seronegative calves were intravenously inoculated with bluetongue virus (BTV) serotype 10, and two calves were inoculated with a placebo. Cellular association of BTV during viremia was investigated in three of the calves by titrating virus present in plasma and different blood cell fractions at weekly intervals after infection. Viremia persisted 35 to 49 days in individual calves. Virus was transiently isolated from blood mononuclear cells and plasma collected from two of the calves but was consistently isolated from erythrocytes throughout infection of all three animals. Titers of BTV present in the erythrocyte fraction were comparable to those of the unseparated blood cell fraction. Tissue tropism of BTV was determined by viral isolation from tissues collected from calves euthanatized at 1, 2, 3, 4, 5, 7, 14, 28, 42, and 56 (2 calves) days after inoculation with BTV. Tropism was also determined by immunohistochemical staining of selected tissues with an avidin-biotin complex immunoperoxidase staining procedure using three BTV-specific monoclonal antibodies. The BTV infected calves remained healthy throughout the study. Virus was isolated from at least one tissue collected from calves euthanatized at 1 through 28 days after inoculation, but not thereafter. High titers of BTV were present in the lungs, prescapular and mesenteric lymph nodes, thymus, and spleen of calves euthanatized at 1 to 4 days after inoculation, whereas BTV was either not isolated or isolated in low titer from bone marrow collected from these animals.(ABSTRACT TRUNCATED AT 250 WORDS)

Original languageEnglish (US)
Pages (from-to)223-229
Number of pages7
JournalVeterinary Pathology
Volume27
Issue number4
StatePublished - Jul 1990

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Bluetongue virus
Virus Diseases
pathogenesis
calves
infection
Blood Cells
Tropism
Viremia
Viruses
viremia
blood cells
viruses
Erythrocytes
erythrocytes
Staining and Labeling
tissue tropism
Avidin
tropisms
avidin
Biotin

ASJC Scopus subject areas

  • veterinary(all)

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The pathogenesis of experimental bluetongue virus infection of calves. / Maclachlan, Nigel J; Jagels, G.; Rossitto, P. V.; Moore, Peter F; Heidner, H. W.

In: Veterinary Pathology, Vol. 27, No. 4, 07.1990, p. 223-229.

Research output: Contribution to journalArticle

Maclachlan, NJ, Jagels, G, Rossitto, PV, Moore, PF & Heidner, HW 1990, 'The pathogenesis of experimental bluetongue virus infection of calves.', Veterinary Pathology, vol. 27, no. 4, pp. 223-229.
Maclachlan, Nigel J ; Jagels, G. ; Rossitto, P. V. ; Moore, Peter F ; Heidner, H. W. / The pathogenesis of experimental bluetongue virus infection of calves. In: Veterinary Pathology. 1990 ; Vol. 27, No. 4. pp. 223-229.
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