The ovulated ovum of the horse

cytology of nonfertilized ova to pronuclear stage ova.

A. C. Enders, Irwin Liu, J. Bowers, K. C. Lantz, S. Schlafke, S. Suarez

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Fertilization and early development in the horse were studied by recovering oviductal ova at various times after postovulatory mating. Ova collected between 7 and 22 h post coitum (pc) were examined for evidence of fertilizing sperm, cellular changes accompanying fertilization, and pronuclear development. Five ova collected between 7 and 9 h pc contained a marginal metaphase plate, but had no indication of sperm components; three of these, however, showed reduced numbers of cortical granules. Two activated ova (10 and 14 h pc) were in telophase of the second meiotic division, following incorporation of the fertilizing sperm. The fertilizing sperm was situated in a slight elevation; the nucleus was expanding but lacked a nuclear envelope. The pronuclear stage in the horse began as early as 12 h pc, and lasted at least until 21 h pc. Sperm tail remnants were seen in 5 of 7 pronuclear-stage ova, although the crowding of the cytoplasm with clusters of lipid and vacuoles made discerning sperm tail remnants difficult. The spindles of the metaphase stage of the second meiotic division were oriented radially, that is, at right angles to the cell surface, in all but one ovum, so this orientation is not a response to fertilization.

Original languageEnglish (US)
Pages (from-to)453-466
Number of pages14
JournalBiology of Reproduction
Volume37
Issue number2
DOIs
StatePublished - Jan 1 1987

Fingerprint

Horses
Ovum
Cell Biology
Spermatozoa
Fertilization
Sperm Tail
Metaphase
Telophase
Crowding
Nuclear Envelope
Vacuoles
Cytoplasm
Lipids

ASJC Scopus subject areas

  • Cell Biology

Cite this

The ovulated ovum of the horse : cytology of nonfertilized ova to pronuclear stage ova. / Enders, A. C.; Liu, Irwin; Bowers, J.; Lantz, K. C.; Schlafke, S.; Suarez, S.

In: Biology of Reproduction, Vol. 37, No. 2, 01.01.1987, p. 453-466.

Research output: Contribution to journalArticle

Enders, A. C. ; Liu, Irwin ; Bowers, J. ; Lantz, K. C. ; Schlafke, S. ; Suarez, S. / The ovulated ovum of the horse : cytology of nonfertilized ova to pronuclear stage ova. In: Biology of Reproduction. 1987 ; Vol. 37, No. 2. pp. 453-466.
@article{75f35671aeda4a30b2f88193b21fc381,
title = "The ovulated ovum of the horse: cytology of nonfertilized ova to pronuclear stage ova.",
abstract = "Fertilization and early development in the horse were studied by recovering oviductal ova at various times after postovulatory mating. Ova collected between 7 and 22 h post coitum (pc) were examined for evidence of fertilizing sperm, cellular changes accompanying fertilization, and pronuclear development. Five ova collected between 7 and 9 h pc contained a marginal metaphase plate, but had no indication of sperm components; three of these, however, showed reduced numbers of cortical granules. Two activated ova (10 and 14 h pc) were in telophase of the second meiotic division, following incorporation of the fertilizing sperm. The fertilizing sperm was situated in a slight elevation; the nucleus was expanding but lacked a nuclear envelope. The pronuclear stage in the horse began as early as 12 h pc, and lasted at least until 21 h pc. Sperm tail remnants were seen in 5 of 7 pronuclear-stage ova, although the crowding of the cytoplasm with clusters of lipid and vacuoles made discerning sperm tail remnants difficult. The spindles of the metaphase stage of the second meiotic division were oriented radially, that is, at right angles to the cell surface, in all but one ovum, so this orientation is not a response to fertilization.",
author = "Enders, {A. C.} and Irwin Liu and J. Bowers and Lantz, {K. C.} and S. Schlafke and S. Suarez",
year = "1987",
month = "1",
day = "1",
doi = "10.1095/biolreprod37.2.453",
language = "English (US)",
volume = "37",
pages = "453--466",
journal = "Biology of Reproduction",
issn = "0006-3363",
publisher = "Society for the Study of Reproduction",
number = "2",

}

TY - JOUR

T1 - The ovulated ovum of the horse

T2 - cytology of nonfertilized ova to pronuclear stage ova.

AU - Enders, A. C.

AU - Liu, Irwin

AU - Bowers, J.

AU - Lantz, K. C.

AU - Schlafke, S.

AU - Suarez, S.

PY - 1987/1/1

Y1 - 1987/1/1

N2 - Fertilization and early development in the horse were studied by recovering oviductal ova at various times after postovulatory mating. Ova collected between 7 and 22 h post coitum (pc) were examined for evidence of fertilizing sperm, cellular changes accompanying fertilization, and pronuclear development. Five ova collected between 7 and 9 h pc contained a marginal metaphase plate, but had no indication of sperm components; three of these, however, showed reduced numbers of cortical granules. Two activated ova (10 and 14 h pc) were in telophase of the second meiotic division, following incorporation of the fertilizing sperm. The fertilizing sperm was situated in a slight elevation; the nucleus was expanding but lacked a nuclear envelope. The pronuclear stage in the horse began as early as 12 h pc, and lasted at least until 21 h pc. Sperm tail remnants were seen in 5 of 7 pronuclear-stage ova, although the crowding of the cytoplasm with clusters of lipid and vacuoles made discerning sperm tail remnants difficult. The spindles of the metaphase stage of the second meiotic division were oriented radially, that is, at right angles to the cell surface, in all but one ovum, so this orientation is not a response to fertilization.

AB - Fertilization and early development in the horse were studied by recovering oviductal ova at various times after postovulatory mating. Ova collected between 7 and 22 h post coitum (pc) were examined for evidence of fertilizing sperm, cellular changes accompanying fertilization, and pronuclear development. Five ova collected between 7 and 9 h pc contained a marginal metaphase plate, but had no indication of sperm components; three of these, however, showed reduced numbers of cortical granules. Two activated ova (10 and 14 h pc) were in telophase of the second meiotic division, following incorporation of the fertilizing sperm. The fertilizing sperm was situated in a slight elevation; the nucleus was expanding but lacked a nuclear envelope. The pronuclear stage in the horse began as early as 12 h pc, and lasted at least until 21 h pc. Sperm tail remnants were seen in 5 of 7 pronuclear-stage ova, although the crowding of the cytoplasm with clusters of lipid and vacuoles made discerning sperm tail remnants difficult. The spindles of the metaphase stage of the second meiotic division were oriented radially, that is, at right angles to the cell surface, in all but one ovum, so this orientation is not a response to fertilization.

UR - http://www.scopus.com/inward/record.url?scp=0023408381&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023408381&partnerID=8YFLogxK

U2 - 10.1095/biolreprod37.2.453

DO - 10.1095/biolreprod37.2.453

M3 - Article

VL - 37

SP - 453

EP - 466

JO - Biology of Reproduction

JF - Biology of Reproduction

SN - 0006-3363

IS - 2

ER -