Abstract
In the adult rat brain, MAP 2 is a high-molecular weight protein that is highly concentrated in dendrites. Immunoblots of homogenates of developing rat brain have indicated that a low-molecular weight form of MAP 2, MAP 2 c, is transiently expressed as the brain is undergoing morphogenesis. Using MAP 2-specific monoclonal antibodies, we have demonstrated that the compartmentalization of high-molecular weight MAP 2 and the developmental regulation of MAP 2 are conserved in mammalian, avian, and amphibian brain. We have also determined the distribution of MAP 2 c in developing neuronal tissue. MAP 2 c appears before high-molecular weight MAP 2 in developing neurons, and in contrast to the dendrite-specific high-molecular weight forms of MAP 2, MAP 2 c is present in axons and glia. We have also shown that MAP 2 c is present in the adult rat retina, where it is concentrated in regenerative photosensitive cells. The transient expression of MAP 2 c in the developing brain of three species as well as in adult photosensitive cells suggests a role for this protein in neurite growth and plasticity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 195-199 |
| Number of pages | 5 |
| Journal | Protoplasma |
| Volume | 145 |
| Issue number | 2-3 |
| DOIs | |
| State | Published - Jun 1988 |
| Externally published | Yes |
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Keywords
- Development
- Microtubule-associated proteins
- Quail
- Rat
- Xenopus laevis
ASJC Scopus subject areas
- Cell Biology
- Plant Science
Cite this
The ontogeny and phylogenetic conservation of MAP 2 forms. / Tucker, Richard P; Viereck, C.; Matus, A. I.
In: Protoplasma, Vol. 145, No. 2-3, 06.1988, p. 195-199.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The ontogeny and phylogenetic conservation of MAP 2 forms
AU - Tucker, Richard P
AU - Viereck, C.
AU - Matus, A. I.
PY - 1988/6
Y1 - 1988/6
N2 - In the adult rat brain, MAP 2 is a high-molecular weight protein that is highly concentrated in dendrites. Immunoblots of homogenates of developing rat brain have indicated that a low-molecular weight form of MAP 2, MAP 2 c, is transiently expressed as the brain is undergoing morphogenesis. Using MAP 2-specific monoclonal antibodies, we have demonstrated that the compartmentalization of high-molecular weight MAP 2 and the developmental regulation of MAP 2 are conserved in mammalian, avian, and amphibian brain. We have also determined the distribution of MAP 2 c in developing neuronal tissue. MAP 2 c appears before high-molecular weight MAP 2 in developing neurons, and in contrast to the dendrite-specific high-molecular weight forms of MAP 2, MAP 2 c is present in axons and glia. We have also shown that MAP 2 c is present in the adult rat retina, where it is concentrated in regenerative photosensitive cells. The transient expression of MAP 2 c in the developing brain of three species as well as in adult photosensitive cells suggests a role for this protein in neurite growth and plasticity.
AB - In the adult rat brain, MAP 2 is a high-molecular weight protein that is highly concentrated in dendrites. Immunoblots of homogenates of developing rat brain have indicated that a low-molecular weight form of MAP 2, MAP 2 c, is transiently expressed as the brain is undergoing morphogenesis. Using MAP 2-specific monoclonal antibodies, we have demonstrated that the compartmentalization of high-molecular weight MAP 2 and the developmental regulation of MAP 2 are conserved in mammalian, avian, and amphibian brain. We have also determined the distribution of MAP 2 c in developing neuronal tissue. MAP 2 c appears before high-molecular weight MAP 2 in developing neurons, and in contrast to the dendrite-specific high-molecular weight forms of MAP 2, MAP 2 c is present in axons and glia. We have also shown that MAP 2 c is present in the adult rat retina, where it is concentrated in regenerative photosensitive cells. The transient expression of MAP 2 c in the developing brain of three species as well as in adult photosensitive cells suggests a role for this protein in neurite growth and plasticity.
KW - Development
KW - Microtubule-associated proteins
KW - Quail
KW - Rat
KW - Xenopus laevis
UR - http://www.scopus.com/inward/record.url?scp=34250091464&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34250091464&partnerID=8YFLogxK
U2 - 10.1007/BF01349359
DO - 10.1007/BF01349359
M3 - Article
AN - SCOPUS:34250091464
VL - 145
SP - 195
EP - 199
JO - Protoplasma
JF - Protoplasma
SN - 0033-183X
IS - 2-3
ER -