The one-pot multienzyme (OPME) synthesis of human blood group H antigens and a human milk oligosaccharide (HMOS) with highly active Thermosynechococcus elongatus α1-2-fucosyltransferase

Chao Zhao, Yijing Wu, Hai Yu, Ishita M. Shah, Yanhong Li, Jie Zeng, Bin Liu, David A. Mills, Xi Chen

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

A novel α1-2-fucosyltransferase from Thermosynechococcus elongatus BP-1 (Te2FT) with high fucosyltransferase activity and low donor hydrolysis activity was discovered and characterized. It was used in an efficient one-pot multienzyme (OPME) fucosylation system for the high-yield synthesis of human blood group H antigens containing β1-3-linked galactosides and an important human milk oligosaccharide (HMOS) lacto-N-fucopentaose I (LNFP I) on preparative and gram scales. LNFP I was shown to be selectively consumed by Bifidobacterium longum subsp. infantis but not Bifidobacterium animalis subsp. lactis and is a potential prebiotic.

Original languageEnglish (US)
Pages (from-to)3899-3902
Number of pages4
JournalChemical Communications
Volume52
Issue number20
DOIs
Publication statusPublished - 2016

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ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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