The one-pot multienzyme (OPME) synthesis of human blood group H antigens and a human milk oligosaccharide (HMOS) with highly active Thermosynechococcus elongatus α1-2-fucosyltransferase

Chao Zhao, Yijing Wu, Hai Yu, Ishita M. Shah, Yanhong Li, Jie Zeng, Bin Liu, David A. Mills, Xi Chen

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A novel α1-2-fucosyltransferase from Thermosynechococcus elongatus BP-1 (Te2FT) with high fucosyltransferase activity and low donor hydrolysis activity was discovered and characterized. It was used in an efficient one-pot multienzyme (OPME) fucosylation system for the high-yield synthesis of human blood group H antigens containing β1-3-linked galactosides and an important human milk oligosaccharide (HMOS) lacto-N-fucopentaose I (LNFP I) on preparative and gram scales. LNFP I was shown to be selectively consumed by Bifidobacterium longum subsp. infantis but not Bifidobacterium animalis subsp. lactis and is a potential prebiotic.

Original languageEnglish (US)
Pages (from-to)3899-3902
Number of pages4
JournalChemical Communications
Volume52
Issue number20
DOIs
StatePublished - 2016

Fingerprint

Oligosaccharides
Antigens
Hydrolysis
Blood
Fucosyltransferases
Galactosides
Prebiotics
lacto-N-fucopentaose I
H antigen
galactoside 2-alpha-L-fucosyltransferase
Human Milk

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

Cite this

The one-pot multienzyme (OPME) synthesis of human blood group H antigens and a human milk oligosaccharide (HMOS) with highly active Thermosynechococcus elongatus α1-2-fucosyltransferase. / Zhao, Chao; Wu, Yijing; Yu, Hai; Shah, Ishita M.; Li, Yanhong; Zeng, Jie; Liu, Bin; Mills, David A.; Chen, Xi.

In: Chemical Communications, Vol. 52, No. 20, 2016, p. 3899-3902.

Research output: Contribution to journalArticle

Zhao, Chao ; Wu, Yijing ; Yu, Hai ; Shah, Ishita M. ; Li, Yanhong ; Zeng, Jie ; Liu, Bin ; Mills, David A. ; Chen, Xi. / The one-pot multienzyme (OPME) synthesis of human blood group H antigens and a human milk oligosaccharide (HMOS) with highly active Thermosynechococcus elongatus α1-2-fucosyltransferase. In: Chemical Communications. 2016 ; Vol. 52, No. 20. pp. 3899-3902.
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AU - Wu, Yijing

AU - Yu, Hai

AU - Shah, Ishita M.

AU - Li, Yanhong

AU - Zeng, Jie

AU - Liu, Bin

AU - Mills, David A.

AU - Chen, Xi

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