The occurrence of subtilase-cytotoxin-encoding genes in environmental Escherichia coli isolated from a Northern California estuary

Maria das Graças C Pereira, Barbara A Byrne, Trân B H Nguyen, David J. Lewis, Edward R Atwill

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The presence of subtilase-cytotoxin-encoding genes was determined in 397 environmental Escherichia coli strains isolated from water, suspended solids, and sediments sampled from different hydrological and environmental conditions in a California estuary. A total of 7 strains (1.76%) were found to harbor subtilase-cytotoxin-encoding genes. Using primers targeting subA only, we generated PCR amplicons from 2 strains; while using primers targeting the 3′ end of SubA downstream to the 5′ end of SubB, amplicons of 232 bp were generated from 5 additional strains. The 556 bp subA sequences were almost identical to that in the subtilase-cytotoxin-positive strain ED 591 (98%), while subAB sequences of 2 non-Shiga-toxigenic strains revealed 100% similarity with the Shiga-toxigenic E. coli O113:H21 strain 98NK2 that was isolated from an outbreak of hemolytic uremic syndrome. Additionally, the serogroup O113:H21 was present in this collection of environmental E. coli, and it was found to harbor stx2d, hra1 that encodes the heat resistant agglutinin 1, and a subAB sequence similar to that in the non-Shiga-toxigenic E. coli subtilase cytotoxin strain ED 591. To further understand potential health risks posed by strains encoding SubAB, future epidemiological studies should consider screening isolates for subAB regardless of the presence of Shiga-toxin-encoding genes.

Original languageEnglish (US)
Pages (from-to)437-441
Number of pages5
JournalCanadian Journal of Microbiology
Volume59
Issue number6
DOIs
StatePublished - Jun 2013

Fingerprint

Estuaries
Cytotoxins
Escherichia coli
Shiga Toxin
Genes
Shiga-Toxigenic Escherichia coli
Hemolytic-Uremic Syndrome
Agglutinins
Disease Outbreaks
Epidemiologic Studies
Hot Temperature
Polymerase Chain Reaction
Water
Health

Keywords

  • Environmental E. coli
  • Non-O157 E. coli
  • Shiga toxin
  • Subtilase cytotoxin
  • Surface water

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology
  • Immunology
  • Genetics
  • Molecular Biology

Cite this

The occurrence of subtilase-cytotoxin-encoding genes in environmental Escherichia coli isolated from a Northern California estuary. / Pereira, Maria das Graças C; Byrne, Barbara A; Nguyen, Trân B H; Lewis, David J.; Atwill, Edward R.

In: Canadian Journal of Microbiology, Vol. 59, No. 6, 06.2013, p. 437-441.

Research output: Contribution to journalArticle

@article{f173927d8dce44578de0d3a6eba7ef22,
title = "The occurrence of subtilase-cytotoxin-encoding genes in environmental Escherichia coli isolated from a Northern California estuary",
abstract = "The presence of subtilase-cytotoxin-encoding genes was determined in 397 environmental Escherichia coli strains isolated from water, suspended solids, and sediments sampled from different hydrological and environmental conditions in a California estuary. A total of 7 strains (1.76{\%}) were found to harbor subtilase-cytotoxin-encoding genes. Using primers targeting subA only, we generated PCR amplicons from 2 strains; while using primers targeting the 3′ end of SubA downstream to the 5′ end of SubB, amplicons of 232 bp were generated from 5 additional strains. The 556 bp subA sequences were almost identical to that in the subtilase-cytotoxin-positive strain ED 591 (98{\%}), while subAB sequences of 2 non-Shiga-toxigenic strains revealed 100{\%} similarity with the Shiga-toxigenic E. coli O113:H21 strain 98NK2 that was isolated from an outbreak of hemolytic uremic syndrome. Additionally, the serogroup O113:H21 was present in this collection of environmental E. coli, and it was found to harbor stx2d, hra1 that encodes the heat resistant agglutinin 1, and a subAB sequence similar to that in the non-Shiga-toxigenic E. coli subtilase cytotoxin strain ED 591. To further understand potential health risks posed by strains encoding SubAB, future epidemiological studies should consider screening isolates for subAB regardless of the presence of Shiga-toxin-encoding genes.",
keywords = "Environmental E. coli, Non-O157 E. coli, Shiga toxin, Subtilase cytotoxin, Surface water",
author = "Pereira, {Maria das Gra{\cc}as C} and Byrne, {Barbara A} and Nguyen, {Tr{\^a}n B H} and Lewis, {David J.} and Atwill, {Edward R}",
year = "2013",
month = "6",
doi = "10.1139/cjm-2012-0606",
language = "English (US)",
volume = "59",
pages = "437--441",
journal = "Canadian Journal of Microbiology",
issn = "0008-4166",
publisher = "National Research Council of Canada",
number = "6",

}

TY - JOUR

T1 - The occurrence of subtilase-cytotoxin-encoding genes in environmental Escherichia coli isolated from a Northern California estuary

AU - Pereira, Maria das Graças C

AU - Byrne, Barbara A

AU - Nguyen, Trân B H

AU - Lewis, David J.

AU - Atwill, Edward R

PY - 2013/6

Y1 - 2013/6

N2 - The presence of subtilase-cytotoxin-encoding genes was determined in 397 environmental Escherichia coli strains isolated from water, suspended solids, and sediments sampled from different hydrological and environmental conditions in a California estuary. A total of 7 strains (1.76%) were found to harbor subtilase-cytotoxin-encoding genes. Using primers targeting subA only, we generated PCR amplicons from 2 strains; while using primers targeting the 3′ end of SubA downstream to the 5′ end of SubB, amplicons of 232 bp were generated from 5 additional strains. The 556 bp subA sequences were almost identical to that in the subtilase-cytotoxin-positive strain ED 591 (98%), while subAB sequences of 2 non-Shiga-toxigenic strains revealed 100% similarity with the Shiga-toxigenic E. coli O113:H21 strain 98NK2 that was isolated from an outbreak of hemolytic uremic syndrome. Additionally, the serogroup O113:H21 was present in this collection of environmental E. coli, and it was found to harbor stx2d, hra1 that encodes the heat resistant agglutinin 1, and a subAB sequence similar to that in the non-Shiga-toxigenic E. coli subtilase cytotoxin strain ED 591. To further understand potential health risks posed by strains encoding SubAB, future epidemiological studies should consider screening isolates for subAB regardless of the presence of Shiga-toxin-encoding genes.

AB - The presence of subtilase-cytotoxin-encoding genes was determined in 397 environmental Escherichia coli strains isolated from water, suspended solids, and sediments sampled from different hydrological and environmental conditions in a California estuary. A total of 7 strains (1.76%) were found to harbor subtilase-cytotoxin-encoding genes. Using primers targeting subA only, we generated PCR amplicons from 2 strains; while using primers targeting the 3′ end of SubA downstream to the 5′ end of SubB, amplicons of 232 bp were generated from 5 additional strains. The 556 bp subA sequences were almost identical to that in the subtilase-cytotoxin-positive strain ED 591 (98%), while subAB sequences of 2 non-Shiga-toxigenic strains revealed 100% similarity with the Shiga-toxigenic E. coli O113:H21 strain 98NK2 that was isolated from an outbreak of hemolytic uremic syndrome. Additionally, the serogroup O113:H21 was present in this collection of environmental E. coli, and it was found to harbor stx2d, hra1 that encodes the heat resistant agglutinin 1, and a subAB sequence similar to that in the non-Shiga-toxigenic E. coli subtilase cytotoxin strain ED 591. To further understand potential health risks posed by strains encoding SubAB, future epidemiological studies should consider screening isolates for subAB regardless of the presence of Shiga-toxin-encoding genes.

KW - Environmental E. coli

KW - Non-O157 E. coli

KW - Shiga toxin

KW - Subtilase cytotoxin

KW - Surface water

UR - http://www.scopus.com/inward/record.url?scp=84878787739&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84878787739&partnerID=8YFLogxK

U2 - 10.1139/cjm-2012-0606

DO - 10.1139/cjm-2012-0606

M3 - Article

C2 - 23750960

AN - SCOPUS:84878787739

VL - 59

SP - 437

EP - 441

JO - Canadian Journal of Microbiology

JF - Canadian Journal of Microbiology

SN - 0008-4166

IS - 6

ER -