The neutrophil granule protein cathepsin G activates murine T lymphocytes and upregulates antigen-specific Ig production in mice

Kenji Tani, William J Murphy, Oleg Chertov, Joost J. Oppenheim, Ji Ming Wang

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Cathepsin G is a neutrophil granule derived antimicrobial chymotrypsin-like enzyme. Our previous study showed that cathepsin G induces chemotactic migration of human phagocytic leukocytes and increases random migration of T lymphocytes. In this study, we investigated the capacity of cathepsin G to activate T lymphocytes and to modulate antigen-specific humoral responses in mice. We found that cathepsin G is mitogenic for and induces production of IFN-γ by murine T cells in vitro. Injection of cathepsin G in BALB/c mice immunized with keyhole limpet hemocyanin (KLH) adsorbed to aluminum hydroxide resulted in a significant]fy increased production of KLH-specific IgG1 and IgG2a antibodies. There was a dose-dependent increase in KLH-specific proliferation of lymphocytes from draining lymph nodes from mice treated with KLH and cathepsin G when compared with those treated with KLH alone. Subsequent analysis of IFN-γ and IL-4 release following in vitro restimulation of draining lymph node lymphocytes obtained from KLH-immunized mice suggested that cathepsin G augments KLH-specific Ig antibody production via activation of T cells, presumably involving both Th1 and Th2 pathways. Thus, neutrophil granule cathepsin G, in addition to its capacity to kill microbes and to enhance leukocyte motility, activates T lymphocytes and modulates humoral immunity.

Original languageEnglish (US)
Pages (from-to)971-976
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume282
Issue number4
DOIs
StatePublished - 2001
Externally publishedYes

Keywords

  • Adjuvant
  • Antibody
  • Cathepsin G
  • T cell

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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