The mr 115 kd fiber cell-specific protein is a component of the Lens cytoskeleton

Paul G FitzGerald, Wayne Gottlieb

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Electron microscope level immunocytochemistry was used to localize a lens fiber cell-specific protein with an Mr of 115 kd. Affinity-purified polyclonal antibodies were utilized on sections of detergent-extracted, acrylic-embedded lens cortical fiber cells. Monoclonal antibodies were utilized for pre-embeddment labelling of a subcellular fraction of lens fiber cells generated by homogenization, and high-speed centrifugation. The results indicate that the Mr 115 kd antigen is a component of the lens fiber cell cytoskeleton, specifically the beaded filament (BF), a cytoskeletal element thought to be unique to the differentiated lens fiber cell.

Original languageEnglish (US)
Pages (from-to)801-811
Number of pages11
JournalCurrent Eye Research
Volume8
Issue number8
DOIs
StatePublished - 1989

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Cytoskeleton
Lenses
Proteins
Subcellular Fractions
Centrifugation
Detergents
Immunohistochemistry
Monoclonal Antibodies
Electrons
Antigens
Antibodies

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

The mr 115 kd fiber cell-specific protein is a component of the Lens cytoskeleton. / FitzGerald, Paul G; Gottlieb, Wayne.

In: Current Eye Research, Vol. 8, No. 8, 1989, p. 801-811.

Research output: Contribution to journalArticle

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