The mouse transition protein 1 gene contains a B1 repetitive element and is located on chromosome 1

Pamela C. Yelick, Christine Kozak, Yunhee K. Kwon, Michael F Seldin, Norman B. Hecht

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The gene for mouse transition protein 1 (mTP1) was isolated, sequenced, and chromosomally mapped. The nucleotide sequence of 1895 bp of a 6.4-kb mTP1 genomic subclone was determined to include 788 bp of 5′ flanking region, 564 bp of coding region including a 396-bp intron and a TAA stop codon, and 543 bp of 3′ flanking region. The mTP1 gene contains a B1 repeat sequence within the only intron of the gene. The transcriptional start site of the mTP1 mRNA was determined to be located 31 bases upstream of the ATG translational start codon. Southern blot analysis demonstrated the presence of sequences homologous to the mTP1 cDNA in the genomes of the rat, hamster, bull, boar, dog, horse, ram, human, and two marsupials (the American opossum and Monodelphis), suggesting that the mTP1 gene sequence is widely conserved. The TP1 gene has been mapped by analysis of restriction fragment length variants (RFLV) in an interspecific backcross to a position 0.7 ± 0.4 cM telomeric of Mylf and 1.2 ± 0.5 cM centromeric of Vil on mouse chromosome 1.

Original languageEnglish (US)
Pages (from-to)687-694
Number of pages8
JournalGenomics
Volume11
Issue number3
DOIs
StatePublished - 1991
Externally publishedYes

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Chromosomes, Human, Pair 1
Genes
Terminator Codon
Introns
Monodelphis
3' Flanking Region
Opossums
Marsupialia
Initiator Codon
5' Flanking Region
Sequence Homology
Southern Blotting
Cricetinae
Horses
spermatid transition proteins
Complementary DNA
Genome
Dogs
Messenger RNA

ASJC Scopus subject areas

  • Genetics

Cite this

The mouse transition protein 1 gene contains a B1 repetitive element and is located on chromosome 1. / Yelick, Pamela C.; Kozak, Christine; Kwon, Yunhee K.; Seldin, Michael F; Hecht, Norman B.

In: Genomics, Vol. 11, No. 3, 1991, p. 687-694.

Research output: Contribution to journalArticle

Yelick, Pamela C. ; Kozak, Christine ; Kwon, Yunhee K. ; Seldin, Michael F ; Hecht, Norman B. / The mouse transition protein 1 gene contains a B1 repetitive element and is located on chromosome 1. In: Genomics. 1991 ; Vol. 11, No. 3. pp. 687-694.
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