The mouse muscle creatine kinase promoter faithfully drives reporter gene expression in transgenic Xenopus laevis

Wayland Lim, Eric S. Neff, John Furlow

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Developing Xenopus laevis experience two periods of muscle differentiation, once during embryogenesis and again at metamorphosis. During metamorphosis, thyroid hormone induces both muscle growth in the limbs and muscle death in the tail. In mammals, the muscle creatine kinase (MCK) gene is activated during the differentiation from myoblasts to myocytes and has served as both a marker for muscle development and to drive transgene expression in transgenic mice. Transcriptional control elements are generally highly conserved throughout evolution, potentially allowing mouse promoter use in transgenic X. laevis. This paper compares endogenous X. laevis MCK gene expression and the mouse MCK (mMCK) promoter driving a green fluorescent protein reporter in transgenic X. laevis. The mMCK promoter demonstrated strong skeletal muscle-specific transgene expression in both the juvenile tadpole and adult frog. Therefore, our results clearly demonstrate the functional conservation of regulatory sequences in vertebrate muscle gene promoters and illustrate the utility of using X. laevis transgenesis for detailed comparative study of mammalian promoter activity in vivo.

Original languageEnglish (US)
Pages (from-to)79-86
Number of pages8
JournalPhysiological Genomics
Volume18
DOIs
StatePublished - Oct 1 2004

Fingerprint

MM Form Creatine Kinase
Xenopus laevis
Reporter Genes
Gene Expression
Muscles
Transgenes
Gene Transfer Techniques
Muscle Development
Myoblasts
Green Fluorescent Proteins
Thyroid Hormones
Anura
Muscle Cells
Transgenic Mice
Genes
Embryonic Development
Larva
Vertebrates
Tail
Mammals

Keywords

  • Frog
  • Gene expression
  • Green fluorescent protein
  • Transgenesis

ASJC Scopus subject areas

  • Physiology
  • Genetics

Cite this

The mouse muscle creatine kinase promoter faithfully drives reporter gene expression in transgenic Xenopus laevis. / Lim, Wayland; Neff, Eric S.; Furlow, John.

In: Physiological Genomics, Vol. 18, 01.10.2004, p. 79-86.

Research output: Contribution to journalArticle

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