The mouse fas-ligand gene is mutated in gld mice and is part of a TNF family gene cluster

David H. Lynch, Mark L. Watson, Mark R. Alderson, Peter R. Baum, Robert E. Miller, Teresa Tough, Marylou Gibson, Terri Davis-Smith, Craig A. Smiths, Kent Hunter, Deepti Bhat, Wenie Din, Raymond G. Goodwin, Michael F Seldin

Research output: Contribution to journalArticlepeer-review

319 Scopus citations


The gene for the mouse Fas ligand was cloned and its chromosomal position determined. Fasl was tightly linked to gld (no crossovers in 567 meiotic events) on mouse chromosome 1 and closely linked with a novel member of the same TNF family of ligands, the 0x40 ligand (0x401,1 crossover in 567 meiotic events). Southern blot analysis did not reveal any difference between the Fasl gene from gld and wild-type mice and levels of Fasl mRNA transcripts were similar in PMA and ionomycin induced wild-type and coisogenic gld T cells. Sequence analysis of the gld gene indicated a single amino acid change (Phe Leu) in the COOH terminal portion of this type II transmembrane protein, and COS cells transfected with Fasl cDNA from gld mice failed to induce apoptosis of Fas-expressing target cells. Thus, the data demonstrate that the gld phenotype is the result of a point mutation in the Fasl gene and that Fasl is part of a complex of ligands structurally related to TNF mapping within a small region of mouse chromosome.

Original languageEnglish (US)
Pages (from-to)131-136
Number of pages6
Issue number2
StatePublished - 1994
Externally publishedYes

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Infectious Diseases


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