The mosquito Anopheles stephensi limits malaria parasite development with inducible synthesis of nitric oxide

Shirley Luckhart, Yoram Vodovotz, Liwang Ciu, Ronald Rosenberg

Research output: Contribution to journalArticle

329 Scopus citations

Abstract

We have discovered that the mosquito Anopheles stephensi, a natural vector of human malaria, limits parasite development with inducible synthesis of nitric oxide (NO). Elevated expression of A. stephensi NO synthase (NOS), which is highly homologous to characterized NOS genes, was detected in the midgut and carcass soon after invasion of the midgut by Plasmodium. Early induction is likely primed by bacterial growth in the blood meal. Later increases in A. stephensi NOS expression and enzyme activity occurred at the beginning of sporozoite release. Circulating levels of nitrite/nitrate, end- products of NO synthesis, were significantly higher in Plasmodium-infected mosquitoes. Dietary provision of the NOS substrate L-arginine reduced Plasmodium infections in A. stephensi. In contrast, dietary provision of a NOS inhibitor significantly increased parasite numbers in infected mosquitoes, confirming that A. stephensi limits Plasmodium development with NO.

Original languageEnglish (US)
Pages (from-to)5700-5705
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume95
Issue number10
DOIs
StatePublished - May 12 1998
Externally publishedYes

ASJC Scopus subject areas

  • Genetics
  • General

Fingerprint Dive into the research topics of 'The mosquito Anopheles stephensi limits malaria parasite development with inducible synthesis of nitric oxide'. Together they form a unique fingerprint.

  • Cite this