Abstract
To characterize flanking regions of the mbx operon in Moraxella bovis, DNA surrounding mbxCABDtolC was sequenced in haemolytic and nonhaemolytic strains of M. bovis. In two haemolytic strains of M. bovis, the mbx operon, including the adjacent M. bovis tolC orthologue, was flanked by approximately 700 bp imperfect repeats. Nonhaemolytic strains of M. bovis had only one or no such repeats, as well as ORFs identical to those flanking the repeats from haemolytic M. bovis. Two nonhaemolytic strains also contained ORFs with deduced amino acid sequence similarity to bacterial araJ genes. The G + C content of the mbxCABDtolC gene region was lower than the flanking regions. The genetic organization and G + C content of mbxCABDtolC genes, and flanking repeats in haemolytic M. bovis, as well as the presence or absence of flanking repeats in nonhaemolytic M. bovis, suggests that this RTX operon is located on a mobile genetic element, and supports the designation of this region as a pathogenicity island, which is believed to be the first such element demonstrated in M. bovis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 443-449 |
| Number of pages | 7 |
| Journal | Journal of Medical Microbiology |
| Volume | 55 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 2006 |
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ASJC Scopus subject areas
- Microbiology (medical)
- Microbiology
Cite this
The Moraxella bovis RTX toxin locus mbx defines a pathogenicity island. / Hess, John F.; Angelos, John A.
In: Journal of Medical Microbiology, Vol. 55, No. 4, 04.2006, p. 443-449.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The Moraxella bovis RTX toxin locus mbx defines a pathogenicity island
AU - Hess, John F.
AU - Angelos, John A
PY - 2006/4
Y1 - 2006/4
N2 - To characterize flanking regions of the mbx operon in Moraxella bovis, DNA surrounding mbxCABDtolC was sequenced in haemolytic and nonhaemolytic strains of M. bovis. In two haemolytic strains of M. bovis, the mbx operon, including the adjacent M. bovis tolC orthologue, was flanked by approximately 700 bp imperfect repeats. Nonhaemolytic strains of M. bovis had only one or no such repeats, as well as ORFs identical to those flanking the repeats from haemolytic M. bovis. Two nonhaemolytic strains also contained ORFs with deduced amino acid sequence similarity to bacterial araJ genes. The G + C content of the mbxCABDtolC gene region was lower than the flanking regions. The genetic organization and G + C content of mbxCABDtolC genes, and flanking repeats in haemolytic M. bovis, as well as the presence or absence of flanking repeats in nonhaemolytic M. bovis, suggests that this RTX operon is located on a mobile genetic element, and supports the designation of this region as a pathogenicity island, which is believed to be the first such element demonstrated in M. bovis.
AB - To characterize flanking regions of the mbx operon in Moraxella bovis, DNA surrounding mbxCABDtolC was sequenced in haemolytic and nonhaemolytic strains of M. bovis. In two haemolytic strains of M. bovis, the mbx operon, including the adjacent M. bovis tolC orthologue, was flanked by approximately 700 bp imperfect repeats. Nonhaemolytic strains of M. bovis had only one or no such repeats, as well as ORFs identical to those flanking the repeats from haemolytic M. bovis. Two nonhaemolytic strains also contained ORFs with deduced amino acid sequence similarity to bacterial araJ genes. The G + C content of the mbxCABDtolC gene region was lower than the flanking regions. The genetic organization and G + C content of mbxCABDtolC genes, and flanking repeats in haemolytic M. bovis, as well as the presence or absence of flanking repeats in nonhaemolytic M. bovis, suggests that this RTX operon is located on a mobile genetic element, and supports the designation of this region as a pathogenicity island, which is believed to be the first such element demonstrated in M. bovis.
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U2 - 10.1099/jmm.0.46366-0
DO - 10.1099/jmm.0.46366-0
M3 - Article
C2 - 16533993
AN - SCOPUS:33645468020
VL - 55
SP - 443
EP - 449
JO - Journal of Medical Microbiology
JF - Journal of Medical Microbiology
SN - 0022-2615
IS - 4
ER -