The mismatch repair system contributes to meiotic sterility in an interspecific yeast hybrid

Neil Hunter, Scott R. Chambers, Edward J. Louis, Rhona H. Borts

Research output: Contribution to journalArticle

224 Scopus citations

Abstract

The mismatch repair system is the major barrier to genetic recombination during interspecific sexual conjugation in prokaryotes. The existence of this antirecombination activity has implications for theories of evolution and the isolation of species. To determine if this phenomenon occurs in eukaryotes, the effect of a deficiency of mismatch repair on the meiotic sterility of an interspecific hybrid of Saccharomyces cerevisiae and the closely related species Saccharomyces paradoxus was examined. The results demonstrate that the rare viable spores from these hybrids have high frequencies of aneuploidy and low frequencies of genetic exchange. Hybrids lacking the mismatch repair genes PMS1 or MSH2 display increased meiotic recombination, decreased chromosome non-disjunction and improved spore viability. These observations are consistent with the proposal that the mismatch repair system is an element of the genetic barrier between eukaryotic species. We suggest that an anti-recombination activity during meiosis contributes towards the establishment of post-zygotic species barriers.

Original languageEnglish (US)
Pages (from-to)1726-1733
Number of pages8
JournalEMBO Journal
Volume15
Issue number7
StatePublished - Apr 1 1996
Externally publishedYes

Keywords

  • Meiosis
  • Mismatch repair
  • Non-disjunction
  • Recombination
  • Speciation

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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    Hunter, N., Chambers, S. R., Louis, E. J., & Borts, R. H. (1996). The mismatch repair system contributes to meiotic sterility in an interspecific yeast hybrid. EMBO Journal, 15(7), 1726-1733.