The metabotropic glutamate agonist 2-amino-4-phosphonobutyric acid (APB) does not activate currents in postnatal retinal ganglion cells

Lauren C. Liets, Leo M. Chalupa

Research output: Contribution to journalArticlepeer-review

Abstract

WHEREAS in the mature retina the glutamate agonist 2-amino-4-phosphonobutyric acid (APB) selectively activates currents in rod bipolar and ON-cone bipolar cells, recent molecular studies have suggested the possibility of a transient appearance of the APB-sensitive receptor in developing retinal ganglion cells. In the present study the whole-cell and perforated variations of the patch-clamp method were employed to assess the responsivity of postnatal cat retinal ganglion cells to APB. Recently, APB treatment has been shown in our laboratory to block the normal stratification of retinal ganglion cell dendrites into ON and OFF sublaminae of the inner plexiform layer. Although application of this glutamate agonist elicited inward sustained currents, amino acid analysis revealed that the APB product (RBI) was contaminated by 8% glycine. In subsequent experiments applications of uncontaminated APB (Cal Biochem) never yielded responses in postnatal retinal ganglion cells which displayed normal currents to other glutamate agonists. The findings do not support the notion of transient expression of APB receptors in retinal ganglion cells during the development period studied.

Original languageEnglish (US)
Pages (from-to)2873-2877
Number of pages5
JournalNeuroReport
Volume7
Issue number18
DOIs
StatePublished - 1996

Keywords

  • AP-4
  • APB
  • Glutamate
  • Retinal ganglion cell
  • Stratification

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'The metabotropic glutamate agonist 2-amino-4-phosphonobutyric acid (APB) does not activate currents in postnatal retinal ganglion cells'. Together they form a unique fingerprint.

Cite this