The metabolome of [2-14C](-)-epicatechin in humans

Implications for the assessment of efficacy, safety, and mechanisms of action of polyphenolic bioactives

Javier I. Ottaviani, Gina Borges, Tony Y. Momma, Jeremy P E Spencer, Carl L Keen, Alan Crozier, Hagen Schroeter

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Diet is a major life style factor affecting human health, thus emphasizing the need for evidence-based dietary guidelines for primary disease prevention. While current recommendations promote intake of fruit and vegetables, we have limited understanding of plant-derived bioactive food constituents other than those representing the small number of essential nutrients and minerals. This limited understanding can be attributed to some extent to a lack of fundamental data describing the absorption, distribution, metabolism and excretion (ADME) of bioactive compounds. Consequently, we selected the flavanol (-)-epicatechin (EC) as an example of a widely studied bioactive food constituent and investigated the ADME of [2-14C](-)-epicatechin (300 μCi, 60 mg) in humans (n = 8). We demonstrated that 82 ± 5% of ingested EC was absorbed. We also established pharmacokinetic profiles and identified and quantified >20 different metabolites. The gut microbiome proved to be a key driver of EC metabolism. Furthermore, we noted striking species-dependent differences in the metabolism of EC, an insight with significant consequences for investigating the mechanisms of action underlying the beneficial effects of EC. These differences need to be considered when assessing the safety of EC intake in humans. We also identified a potential biomarker for the objective assessment of EC intake that could help to strengthen epidemiological investigations.

Original languageEnglish (US)
Article number29034
JournalScientific Reports
Volume6
DOIs
StatePublished - Jul 1 2016

Fingerprint

Metabolome
Catechin
Safety
Food
Nutrition Policy
Primary Prevention
Vegetables
Minerals
Life Style
Fruit
Pharmacokinetics
Biomarkers
Diet
Health

ASJC Scopus subject areas

  • General

Cite this

The metabolome of [2-14C](-)-epicatechin in humans : Implications for the assessment of efficacy, safety, and mechanisms of action of polyphenolic bioactives. / Ottaviani, Javier I.; Borges, Gina; Momma, Tony Y.; Spencer, Jeremy P E; Keen, Carl L; Crozier, Alan; Schroeter, Hagen.

In: Scientific Reports, Vol. 6, 29034, 01.07.2016.

Research output: Contribution to journalArticle

Ottaviani, Javier I. ; Borges, Gina ; Momma, Tony Y. ; Spencer, Jeremy P E ; Keen, Carl L ; Crozier, Alan ; Schroeter, Hagen. / The metabolome of [2-14C](-)-epicatechin in humans : Implications for the assessment of efficacy, safety, and mechanisms of action of polyphenolic bioactives. In: Scientific Reports. 2016 ; Vol. 6.
@article{1109e54152bb45b884894a28aee6cb30,
title = "The metabolome of [2-14C](-)-epicatechin in humans: Implications for the assessment of efficacy, safety, and mechanisms of action of polyphenolic bioactives",
abstract = "Diet is a major life style factor affecting human health, thus emphasizing the need for evidence-based dietary guidelines for primary disease prevention. While current recommendations promote intake of fruit and vegetables, we have limited understanding of plant-derived bioactive food constituents other than those representing the small number of essential nutrients and minerals. This limited understanding can be attributed to some extent to a lack of fundamental data describing the absorption, distribution, metabolism and excretion (ADME) of bioactive compounds. Consequently, we selected the flavanol (-)-epicatechin (EC) as an example of a widely studied bioactive food constituent and investigated the ADME of [2-14C](-)-epicatechin (300 μCi, 60 mg) in humans (n = 8). We demonstrated that 82 ± 5{\%} of ingested EC was absorbed. We also established pharmacokinetic profiles and identified and quantified >20 different metabolites. The gut microbiome proved to be a key driver of EC metabolism. Furthermore, we noted striking species-dependent differences in the metabolism of EC, an insight with significant consequences for investigating the mechanisms of action underlying the beneficial effects of EC. These differences need to be considered when assessing the safety of EC intake in humans. We also identified a potential biomarker for the objective assessment of EC intake that could help to strengthen epidemiological investigations.",
author = "Ottaviani, {Javier I.} and Gina Borges and Momma, {Tony Y.} and Spencer, {Jeremy P E} and Keen, {Carl L} and Alan Crozier and Hagen Schroeter",
year = "2016",
month = "7",
day = "1",
doi = "10.1038/srep29034",
language = "English (US)",
volume = "6",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - The metabolome of [2-14C](-)-epicatechin in humans

T2 - Implications for the assessment of efficacy, safety, and mechanisms of action of polyphenolic bioactives

AU - Ottaviani, Javier I.

AU - Borges, Gina

AU - Momma, Tony Y.

AU - Spencer, Jeremy P E

AU - Keen, Carl L

AU - Crozier, Alan

AU - Schroeter, Hagen

PY - 2016/7/1

Y1 - 2016/7/1

N2 - Diet is a major life style factor affecting human health, thus emphasizing the need for evidence-based dietary guidelines for primary disease prevention. While current recommendations promote intake of fruit and vegetables, we have limited understanding of plant-derived bioactive food constituents other than those representing the small number of essential nutrients and minerals. This limited understanding can be attributed to some extent to a lack of fundamental data describing the absorption, distribution, metabolism and excretion (ADME) of bioactive compounds. Consequently, we selected the flavanol (-)-epicatechin (EC) as an example of a widely studied bioactive food constituent and investigated the ADME of [2-14C](-)-epicatechin (300 μCi, 60 mg) in humans (n = 8). We demonstrated that 82 ± 5% of ingested EC was absorbed. We also established pharmacokinetic profiles and identified and quantified >20 different metabolites. The gut microbiome proved to be a key driver of EC metabolism. Furthermore, we noted striking species-dependent differences in the metabolism of EC, an insight with significant consequences for investigating the mechanisms of action underlying the beneficial effects of EC. These differences need to be considered when assessing the safety of EC intake in humans. We also identified a potential biomarker for the objective assessment of EC intake that could help to strengthen epidemiological investigations.

AB - Diet is a major life style factor affecting human health, thus emphasizing the need for evidence-based dietary guidelines for primary disease prevention. While current recommendations promote intake of fruit and vegetables, we have limited understanding of plant-derived bioactive food constituents other than those representing the small number of essential nutrients and minerals. This limited understanding can be attributed to some extent to a lack of fundamental data describing the absorption, distribution, metabolism and excretion (ADME) of bioactive compounds. Consequently, we selected the flavanol (-)-epicatechin (EC) as an example of a widely studied bioactive food constituent and investigated the ADME of [2-14C](-)-epicatechin (300 μCi, 60 mg) in humans (n = 8). We demonstrated that 82 ± 5% of ingested EC was absorbed. We also established pharmacokinetic profiles and identified and quantified >20 different metabolites. The gut microbiome proved to be a key driver of EC metabolism. Furthermore, we noted striking species-dependent differences in the metabolism of EC, an insight with significant consequences for investigating the mechanisms of action underlying the beneficial effects of EC. These differences need to be considered when assessing the safety of EC intake in humans. We also identified a potential biomarker for the objective assessment of EC intake that could help to strengthen epidemiological investigations.

UR - http://www.scopus.com/inward/record.url?scp=84976897467&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84976897467&partnerID=8YFLogxK

U2 - 10.1038/srep29034

DO - 10.1038/srep29034

M3 - Article

VL - 6

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 29034

ER -