TY - JOUR
T1 - The metabolic profiles of transgenic cucumber lines vary with different chromosomal locations of the transgene
AU - Tagashira, Norikazu
AU - Plader, Wojciech
AU - Filipecki, Marcin
AU - Yin, Zhimin
AU - Wiśniewska, Anita
AU - Gaj, Paweł
AU - Szwacka, Maria
AU - Fiehn, Oliver
AU - Hoshi, Yoshikazu
AU - Kondo, Katsuhiko
AU - Malepszy, Stefan
PY - 2005
Y1 - 2005
N2 - The metabolic profiles of five transgenic cucumber lines were compared taking into consideration their transgene integration sites. The plants analyzed were homozygous and contained transgenes integrated in a single locus on chromosomes I, II, III or IV. The transgenes were preferentially located in the euchromatic regions. Each of these locations possessed a specific metabolic profile. The number of altered compounds in the transgenic lines varied between 9 and 23 of the 47 metabolites identified. These alterations seem to be specific for each independent transgene integration. However, some changes are common: a decrease in the levels of phenylalanine, aspartate, ethanolamine and pipecolate, and an increase in the level of benzoic acid. The observed effects of transgene introduction are discussed in this paper.
AB - The metabolic profiles of five transgenic cucumber lines were compared taking into consideration their transgene integration sites. The plants analyzed were homozygous and contained transgenes integrated in a single locus on chromosomes I, II, III or IV. The transgenes were preferentially located in the euchromatic regions. Each of these locations possessed a specific metabolic profile. The number of altered compounds in the transgenic lines varied between 9 and 23 of the 47 metabolites identified. These alterations seem to be specific for each independent transgene integration. However, some changes are common: a decrease in the levels of phenylalanine, aspartate, ethanolamine and pipecolate, and an increase in the level of benzoic acid. The observed effects of transgene introduction are discussed in this paper.
KW - Fluorescence in situ Hybridization (FISH)
KW - Metabolite Profiling
KW - Thaumatin II
KW - Transgenic Cucumis sativus
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M3 - Article
C2 - 16341278
AN - SCOPUS:31544444154
VL - 10
SP - 697
EP - 710
JO - Cellular and Molecular Biology Letters
JF - Cellular and Molecular Biology Letters
SN - 1425-8153
IS - 4
ER -