The metabolic profiles of transgenic cucumber lines vary with different chromosomal locations of the transgene

Norikazu Tagashira, Wojciech Plader, Marcin Filipecki, Zhimin Yin, Anita Wiśniewska, Paweł Gaj, Maria Szwacka, Oliver Fiehn, Yoshikazu Hoshi, Katsuhiko Kondo, Stefan Malepszy

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The metabolic profiles of five transgenic cucumber lines were compared taking into consideration their transgene integration sites. The plants analyzed were homozygous and contained transgenes integrated in a single locus on chromosomes I, II, III or IV. The transgenes were preferentially located in the euchromatic regions. Each of these locations possessed a specific metabolic profile. The number of altered compounds in the transgenic lines varied between 9 and 23 of the 47 metabolites identified. These alterations seem to be specific for each independent transgene integration. However, some changes are common: a decrease in the levels of phenylalanine, aspartate, ethanolamine and pipecolate, and an increase in the level of benzoic acid. The observed effects of transgene introduction are discussed in this paper.

Original languageEnglish (US)
Pages (from-to)697-710
Number of pages14
JournalCellular and Molecular Biology Letters
Volume10
Issue number4
StatePublished - 2005
Externally publishedYes

Fingerprint

Cucumis sativus
Metabolome
Transgenes
Ethanolamine
Benzoic Acid
Chromosomes
Metabolites
Phenylalanine
Aspartic Acid

Keywords

  • Fluorescence in situ Hybridization (FISH)
  • Metabolite Profiling
  • Thaumatin II
  • Transgenic Cucumis sativus

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Tagashira, N., Plader, W., Filipecki, M., Yin, Z., Wiśniewska, A., Gaj, P., ... Malepszy, S. (2005). The metabolic profiles of transgenic cucumber lines vary with different chromosomal locations of the transgene. Cellular and Molecular Biology Letters, 10(4), 697-710.

The metabolic profiles of transgenic cucumber lines vary with different chromosomal locations of the transgene. / Tagashira, Norikazu; Plader, Wojciech; Filipecki, Marcin; Yin, Zhimin; Wiśniewska, Anita; Gaj, Paweł; Szwacka, Maria; Fiehn, Oliver; Hoshi, Yoshikazu; Kondo, Katsuhiko; Malepszy, Stefan.

In: Cellular and Molecular Biology Letters, Vol. 10, No. 4, 2005, p. 697-710.

Research output: Contribution to journalArticle

Tagashira, N, Plader, W, Filipecki, M, Yin, Z, Wiśniewska, A, Gaj, P, Szwacka, M, Fiehn, O, Hoshi, Y, Kondo, K & Malepszy, S 2005, 'The metabolic profiles of transgenic cucumber lines vary with different chromosomal locations of the transgene', Cellular and Molecular Biology Letters, vol. 10, no. 4, pp. 697-710.
Tagashira, Norikazu ; Plader, Wojciech ; Filipecki, Marcin ; Yin, Zhimin ; Wiśniewska, Anita ; Gaj, Paweł ; Szwacka, Maria ; Fiehn, Oliver ; Hoshi, Yoshikazu ; Kondo, Katsuhiko ; Malepszy, Stefan. / The metabolic profiles of transgenic cucumber lines vary with different chromosomal locations of the transgene. In: Cellular and Molecular Biology Letters. 2005 ; Vol. 10, No. 4. pp. 697-710.
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