The maternal gene skn-1 encodes a protein that is distributed unequally in early C. elegans embryos

Bruce Bowerman, Bruce W. Draper, Craig C. Mello, James R. Priess

Research output: Contribution to journalArticle

189 Citations (Scopus)

Abstract

The autonomous or cell-intrinsic developmental properties of early embryonic blastomeres in nematodes are thought to result from the action of maternally provided determinants. After the first cleavage of the C. elegans embryo, only the posterior blastomere, P1, has a cell-intrinsic ability to produce pharyngeal cells. The product of the maternal gene skn-1 is required for P1 to produce pharyngeal cells. We show here that the Skn-1 protein is nuclear localized and that P1 appears to accumulate markedly higher levels of Skn-1 protein than its sister, the AB blastomere. We have examined the distribution of Skn-1 protein in embryos from mothers with maternal-effect mutations in the genes mex-1, par-1, and pie-1. These results suggest that mex-1(+) and par-1(+) activities are required for the unequal distribution of the Skn-1 protein and that pie-1(+) activity may function to regulate the activity of Skn-1 protein in the descendants of the posterior blastomere P1.

Original languageEnglish (US)
Pages (from-to)443-452
Number of pages10
JournalCell
Volume74
Issue number3
DOIs
StatePublished - Aug 13 1993
Externally publishedYes

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Blastomeres
Embryonic Structures
Genes
Mothers
Proteins
Nuclear Proteins
Mutation

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

The maternal gene skn-1 encodes a protein that is distributed unequally in early C. elegans embryos. / Bowerman, Bruce; Draper, Bruce W.; Mello, Craig C.; Priess, James R.

In: Cell, Vol. 74, No. 3, 13.08.1993, p. 443-452.

Research output: Contribution to journalArticle

Bowerman, Bruce ; Draper, Bruce W. ; Mello, Craig C. ; Priess, James R. / The maternal gene skn-1 encodes a protein that is distributed unequally in early C. elegans embryos. In: Cell. 1993 ; Vol. 74, No. 3. pp. 443-452.
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