The lysine-specific demethylase 1 is required for cell proliferation in both p53-dependent and -independent manners

Ariane Scoumanne, Xinbin Chen

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

The lysine-specific demethylase 1 (LSD1), a component of several histone deacetylase complexes, plays an important role in chromatin remodeling and transcriptional regulation. Here, we generated multiple cell lines in which LSD1 is inducibly expressed or knocked down and found that LSD1 is required for cell proliferation. In addition, we found that deficiency in LSD1 leads to a partial cell cycle arrest in G2/M and sensitizes cells to growth suppression induced by DNA damage or MDM2 inhibition in a p53-dependent manner. We also showed that LSD1 deficiency delays p53 stabilization induced by DNA damage, leading to a delayed induction of p21 and MDM2. Finally, we performed a microarray study and identified several novel LSD1 target genes, including S100A8, which encodes a calcium-binding protein, and DEK, a proto-oncogene. Taken together, we uncovered that LSD1 has a pro-oncogenic function by modulating pro-survival gene expression and p53 transcriptional activity.

Original languageEnglish (US)
Pages (from-to)15471-15475
Number of pages5
JournalJournal of Biological Chemistry
Volume282
Issue number21
DOIs
StatePublished - May 25 2007

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Cell proliferation
Lysine
Cell Proliferation
DNA Damage
Cells
G2 Phase Cell Cycle Checkpoints
Calcium-Binding Proteins
Histone Deacetylases
Chromatin Assembly and Disassembly
Proto-Oncogenes
DNA
Microarrays
Gene expression
Chromatin
Stabilization
Genes
Gene Expression
Cell Line
Growth

ASJC Scopus subject areas

  • Biochemistry

Cite this

The lysine-specific demethylase 1 is required for cell proliferation in both p53-dependent and -independent manners. / Scoumanne, Ariane; Chen, Xinbin.

In: Journal of Biological Chemistry, Vol. 282, No. 21, 25.05.2007, p. 15471-15475.

Research output: Contribution to journalArticle

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