The LsVe1L allele provides a molecular marker for resistance to Verticillium dahliae race 1 in lettuce

Patrik Inderbitzin, Marilena Christopoulou, Dean Lavelle, Sebastian Reyes-Chin-Wo, Richard W Michelmore, Krishna V. Subbarao, Ivan Simko

Research output: Contribution to journalArticle

Abstract

Background: Verticillium wilt caused by the fungus Verticillium dahliae race 1 is among the top disease concerns for lettuce in the Salinas and Pajaro Valleys of coastal central California. Resistance of lettuce against V. dahliae race 1 was previously mapped to the single dominant Verticillium resistance 1 (Vr1) locus. Lines of tomato resistant to race 1 are known to contain the closely linked Ve1 and Ve2 genes that encode receptor-like proteins with extracellular leucine-rich repeats; the Ve1 and Ve2 proteins act antagonistically to provide resistance against V. dahliae race 1. The Vr1 locus in lettuce contains a cluster of several genes with sequence similarity to the tomato Ve genes. We used genome sequencing and/or PCR screening along with pathogenicity assays of 152 accessions of lettuce to investigate allelic diversity and its relationship to race 1 resistance in lettuce. Results: This approach identified a total of four Ve genes: LsVe1, LsVe2, LsVe3, and LsVe4. The majority of accessions, however, contained a combination of only three of these LsVe genes clustered on chromosomal linkage group 9 (within ~ 25 kb in the resistant cultivar La Brillante and within ~ 127 kb in the susceptible cultivar Salinas). Conclusions: A single allele, LsVe1L, was present in all resistant accessions and absent in all susceptible accessions. This allele can be used as a molecular marker for V. dahliae race 1 resistance in lettuce. A PCR assay for rapid detection of race 1 resistance in lettuce was designed based on nucleotide polymorphisms. Application of this assay allows identification of resistant genotypes in early stages of plant development or at seed-level without time- and labor-intensive testing in the field.

Original languageEnglish (US)
Article number305
JournalBMC Plant Biology
Volume19
Issue number1
DOIs
StatePublished - Jul 10 2019

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Verticillium dahliae
lettuce
alleles
genetic markers
Verticillium
assays
genes
tomatoes
Verticillium wilt
loci
cultivars
linkage groups
multigene family
leucine
plant development
labor
pathogenicity
valleys
proteins
nucleotides

Keywords

  • Genomics
  • Lactuca sativa
  • Marker-assisted selection
  • Plant breeding
  • Wilt resistance

ASJC Scopus subject areas

  • Plant Science

Cite this

Inderbitzin, P., Christopoulou, M., Lavelle, D., Reyes-Chin-Wo, S., Michelmore, R. W., Subbarao, K. V., & Simko, I. (2019). The LsVe1L allele provides a molecular marker for resistance to Verticillium dahliae race 1 in lettuce. BMC Plant Biology, 19(1), [305]. https://doi.org/10.1186/s12870-019-1905-9

The LsVe1L allele provides a molecular marker for resistance to Verticillium dahliae race 1 in lettuce. / Inderbitzin, Patrik; Christopoulou, Marilena; Lavelle, Dean; Reyes-Chin-Wo, Sebastian; Michelmore, Richard W; Subbarao, Krishna V.; Simko, Ivan.

In: BMC Plant Biology, Vol. 19, No. 1, 305, 10.07.2019.

Research output: Contribution to journalArticle

Inderbitzin, P, Christopoulou, M, Lavelle, D, Reyes-Chin-Wo, S, Michelmore, RW, Subbarao, KV & Simko, I 2019, 'The LsVe1L allele provides a molecular marker for resistance to Verticillium dahliae race 1 in lettuce', BMC Plant Biology, vol. 19, no. 1, 305. https://doi.org/10.1186/s12870-019-1905-9
Inderbitzin P, Christopoulou M, Lavelle D, Reyes-Chin-Wo S, Michelmore RW, Subbarao KV et al. The LsVe1L allele provides a molecular marker for resistance to Verticillium dahliae race 1 in lettuce. BMC Plant Biology. 2019 Jul 10;19(1). 305. https://doi.org/10.1186/s12870-019-1905-9
Inderbitzin, Patrik ; Christopoulou, Marilena ; Lavelle, Dean ; Reyes-Chin-Wo, Sebastian ; Michelmore, Richard W ; Subbarao, Krishna V. ; Simko, Ivan. / The LsVe1L allele provides a molecular marker for resistance to Verticillium dahliae race 1 in lettuce. In: BMC Plant Biology. 2019 ; Vol. 19, No. 1.
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abstract = "Background: Verticillium wilt caused by the fungus Verticillium dahliae race 1 is among the top disease concerns for lettuce in the Salinas and Pajaro Valleys of coastal central California. Resistance of lettuce against V. dahliae race 1 was previously mapped to the single dominant Verticillium resistance 1 (Vr1) locus. Lines of tomato resistant to race 1 are known to contain the closely linked Ve1 and Ve2 genes that encode receptor-like proteins with extracellular leucine-rich repeats; the Ve1 and Ve2 proteins act antagonistically to provide resistance against V. dahliae race 1. The Vr1 locus in lettuce contains a cluster of several genes with sequence similarity to the tomato Ve genes. We used genome sequencing and/or PCR screening along with pathogenicity assays of 152 accessions of lettuce to investigate allelic diversity and its relationship to race 1 resistance in lettuce. Results: This approach identified a total of four Ve genes: LsVe1, LsVe2, LsVe3, and LsVe4. The majority of accessions, however, contained a combination of only three of these LsVe genes clustered on chromosomal linkage group 9 (within ~ 25 kb in the resistant cultivar La Brillante and within ~ 127 kb in the susceptible cultivar Salinas). Conclusions: A single allele, LsVe1L, was present in all resistant accessions and absent in all susceptible accessions. This allele can be used as a molecular marker for V. dahliae race 1 resistance in lettuce. A PCR assay for rapid detection of race 1 resistance in lettuce was designed based on nucleotide polymorphisms. Application of this assay allows identification of resistant genotypes in early stages of plant development or at seed-level without time- and labor-intensive testing in the field.",
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AB - Background: Verticillium wilt caused by the fungus Verticillium dahliae race 1 is among the top disease concerns for lettuce in the Salinas and Pajaro Valleys of coastal central California. Resistance of lettuce against V. dahliae race 1 was previously mapped to the single dominant Verticillium resistance 1 (Vr1) locus. Lines of tomato resistant to race 1 are known to contain the closely linked Ve1 and Ve2 genes that encode receptor-like proteins with extracellular leucine-rich repeats; the Ve1 and Ve2 proteins act antagonistically to provide resistance against V. dahliae race 1. The Vr1 locus in lettuce contains a cluster of several genes with sequence similarity to the tomato Ve genes. We used genome sequencing and/or PCR screening along with pathogenicity assays of 152 accessions of lettuce to investigate allelic diversity and its relationship to race 1 resistance in lettuce. Results: This approach identified a total of four Ve genes: LsVe1, LsVe2, LsVe3, and LsVe4. The majority of accessions, however, contained a combination of only three of these LsVe genes clustered on chromosomal linkage group 9 (within ~ 25 kb in the resistant cultivar La Brillante and within ~ 127 kb in the susceptible cultivar Salinas). Conclusions: A single allele, LsVe1L, was present in all resistant accessions and absent in all susceptible accessions. This allele can be used as a molecular marker for V. dahliae race 1 resistance in lettuce. A PCR assay for rapid detection of race 1 resistance in lettuce was designed based on nucleotide polymorphisms. Application of this assay allows identification of resistant genotypes in early stages of plant development or at seed-level without time- and labor-intensive testing in the field.

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