Abstract
Natural killer cell functions are regulated by signals through activating and inhibitory receptors. These receptors belong to the immunoglobulin superfamily or the lectin superfamily. We have previously identified a lectin-like transcript, LLT1, expressed in human NK cells. In the present study, we have generated a monoclonal antibody, L9.7, that specifically binds LLT1 receptor and studied the functional role of LLT1 in human NK cells. Binding of mAb L9.7 to surface LLT1 induced IFN-γ production, but did not modulate cytotoxicity by YT cells, a human NK cell line. We further demonstrate that in resting NK cells as well as in IL-2 activated NK cells LLT1 induced IFN-γ production, but not cytotoxicity. Excess amounts of L9.7 mAb failed to increase natural or antibody-dependent cell-mediated cytolytic activity, whereas minimal amounts achieved maximal production of IFN-γ by YT and activated NK cells. These findings further support the separation of signaling pathways that regulate cytotoxicity and IFN-γ production in resting as well as activated NK cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1157-1163 |
| Number of pages | 7 |
| Journal | Molecular Immunology |
| Volume | 40 |
| Issue number | 16 |
| DOIs | |
| State | Published - Mar 2004 |
| Externally published | Yes |
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Keywords
- Human
- Immune regulation
- LLT1
- Natural killer cells
ASJC Scopus subject areas
- Molecular Biology
- Immunology
Cite this
The LLT1 receptor induces IFN-γ production by human natural killer cells. / Mathew, Porunelloor A.; Chuang, Samuel S.; Vaidya, Swapnil V.; Kumaresan, Pappanaicken R.; Boles, Kent S.; Pham, Hoang Tuan K.
In: Molecular Immunology, Vol. 40, No. 16, 03.2004, p. 1157-1163.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The LLT1 receptor induces IFN-γ production by human natural killer cells
AU - Mathew, Porunelloor A.
AU - Chuang, Samuel S.
AU - Vaidya, Swapnil V.
AU - Kumaresan, Pappanaicken R.
AU - Boles, Kent S.
AU - Pham, Hoang Tuan K
PY - 2004/3
Y1 - 2004/3
N2 - Natural killer cell functions are regulated by signals through activating and inhibitory receptors. These receptors belong to the immunoglobulin superfamily or the lectin superfamily. We have previously identified a lectin-like transcript, LLT1, expressed in human NK cells. In the present study, we have generated a monoclonal antibody, L9.7, that specifically binds LLT1 receptor and studied the functional role of LLT1 in human NK cells. Binding of mAb L9.7 to surface LLT1 induced IFN-γ production, but did not modulate cytotoxicity by YT cells, a human NK cell line. We further demonstrate that in resting NK cells as well as in IL-2 activated NK cells LLT1 induced IFN-γ production, but not cytotoxicity. Excess amounts of L9.7 mAb failed to increase natural or antibody-dependent cell-mediated cytolytic activity, whereas minimal amounts achieved maximal production of IFN-γ by YT and activated NK cells. These findings further support the separation of signaling pathways that regulate cytotoxicity and IFN-γ production in resting as well as activated NK cells.
AB - Natural killer cell functions are regulated by signals through activating and inhibitory receptors. These receptors belong to the immunoglobulin superfamily or the lectin superfamily. We have previously identified a lectin-like transcript, LLT1, expressed in human NK cells. In the present study, we have generated a monoclonal antibody, L9.7, that specifically binds LLT1 receptor and studied the functional role of LLT1 in human NK cells. Binding of mAb L9.7 to surface LLT1 induced IFN-γ production, but did not modulate cytotoxicity by YT cells, a human NK cell line. We further demonstrate that in resting NK cells as well as in IL-2 activated NK cells LLT1 induced IFN-γ production, but not cytotoxicity. Excess amounts of L9.7 mAb failed to increase natural or antibody-dependent cell-mediated cytolytic activity, whereas minimal amounts achieved maximal production of IFN-γ by YT and activated NK cells. These findings further support the separation of signaling pathways that regulate cytotoxicity and IFN-γ production in resting as well as activated NK cells.
KW - Human
KW - Immune regulation
KW - LLT1
KW - Natural killer cells
UR - http://www.scopus.com/inward/record.url?scp=1142285364&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=1142285364&partnerID=8YFLogxK
U2 - 10.1016/j.molimm.2003.11.024
DO - 10.1016/j.molimm.2003.11.024
M3 - Article
C2 - 15104121
AN - SCOPUS:1142285364
VL - 40
SP - 1157
EP - 1163
JO - Molecular Immunology
JF - Molecular Immunology
SN - 0161-5890
IS - 16
ER -