Abstract
Natural killer cell functions are regulated by signals through activating and inhibitory receptors. These receptors belong to the immunoglobulin superfamily or the lectin superfamily. We have previously identified a lectin-like transcript, LLT1, expressed in human NK cells. In the present study, we have generated a monoclonal antibody, L9.7, that specifically binds LLT1 receptor and studied the functional role of LLT1 in human NK cells. Binding of mAb L9.7 to surface LLT1 induced IFN-γ production, but did not modulate cytotoxicity by YT cells, a human NK cell line. We further demonstrate that in resting NK cells as well as in IL-2 activated NK cells LLT1 induced IFN-γ production, but not cytotoxicity. Excess amounts of L9.7 mAb failed to increase natural or antibody-dependent cell-mediated cytolytic activity, whereas minimal amounts achieved maximal production of IFN-γ by YT and activated NK cells. These findings further support the separation of signaling pathways that regulate cytotoxicity and IFN-γ production in resting as well as activated NK cells.
Original language | English (US) |
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Pages (from-to) | 1157-1163 |
Number of pages | 7 |
Journal | Molecular Immunology |
Volume | 40 |
Issue number | 16 |
DOIs | |
State | Published - Mar 2004 |
Externally published | Yes |
Keywords
- Human
- Immune regulation
- LLT1
- Natural killer cells
ASJC Scopus subject areas
- Molecular Biology
- Immunology