TY - JOUR
T1 - The linear excisional wound
T2 - An improved model for human ex vivo wound epithelialization studies
AU - Rizzo, Amilcar Ezequiel
AU - Beckett, Laurel A
AU - Baier, Brian S.
AU - Isseroff, Roslyn Rivkah
PY - 2012/2
Y1 - 2012/2
N2 - Background/purpose: Wound healing is a complex process that involves multiple intercellular and intracellular processes and extracellular interactions. Explanted human skin has been used as a model for the re-epithelialization phase of human wound healing. The currently used standard technique uses a circular punch biopsy tool to make the initial wound. Despite its wide use, the geometry of round wounds makes it difficult to measure them reliably. Methods: Our group has designed a linear wounding tool, and compared the variability in ex vivo human linear and circular wounds. Results: An F test for differences in variances demonstrated that the linear wounds provided a population of wound size measurements that was 50% less variable than that obtained from a group of matched circular wounds. This reduction in variability would provide substantial advantages for the linear wound technique over the circular wound punch technique, by reducing the sample sizes required for comparative studies of factors that alter healing. Conclusion: This linear wounding tool thus provides a method for wounding that is standardized, provides minimal error in wound gap measurements, and is easily reproducible. We demonstrate its utility in an ex vivo model for the controlled investigation of human skin wounds.
AB - Background/purpose: Wound healing is a complex process that involves multiple intercellular and intracellular processes and extracellular interactions. Explanted human skin has been used as a model for the re-epithelialization phase of human wound healing. The currently used standard technique uses a circular punch biopsy tool to make the initial wound. Despite its wide use, the geometry of round wounds makes it difficult to measure them reliably. Methods: Our group has designed a linear wounding tool, and compared the variability in ex vivo human linear and circular wounds. Results: An F test for differences in variances demonstrated that the linear wounds provided a population of wound size measurements that was 50% less variable than that obtained from a group of matched circular wounds. This reduction in variability would provide substantial advantages for the linear wound technique over the circular wound punch technique, by reducing the sample sizes required for comparative studies of factors that alter healing. Conclusion: This linear wounding tool thus provides a method for wounding that is standardized, provides minimal error in wound gap measurements, and is easily reproducible. We demonstrate its utility in an ex vivo model for the controlled investigation of human skin wounds.
KW - Ex vivo skin wound model
KW - Explanted skin model
KW - Organotypic human skin culture
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U2 - 10.1111/j.1600-0846.2011.00528.x
DO - 10.1111/j.1600-0846.2011.00528.x
M3 - Article
C2 - 21605167
AN - SCOPUS:83655163926
VL - 18
SP - 125
EP - 132
JO - Skin Research and Technology
JF - Skin Research and Technology
SN - 0909-752X
IS - 1
ER -