Abstract
Accidental contact with caterpillar bristles causes local symptoms such as severe pain, intense heat, edema, erythema, and pruritus. However, there is little functional evidence to indicate a potential mechanism. In this study, we analyzed the biological characteristics of the crude venom from the larval stage of Latoia consocia living in South-West China. Intraplantar injection of the venom into the hind paws of mice induced severe acute pain behaviors in wild type (WT) mice; the responses were much reduced in TRPV1-deficit (TRPV1 KO) mice. The TRPV1-specific inhibitor, capsazepine, significantly attenuated the pain behaviors. Furthermore, the crude venom evoked strong calcium signals in the dorsal root ganglion (DRG) neurons of WT mice but not those of TRPV1 KO mice. Among the pain-related ion channels we tested, the crude venom only activated the TRPV1 channel. To better understand the venom components, we analyzed the transcriptome of the L. consocia sebaceous gland region. Our study suggests that TRPV1 serves as a primary nociceptor in caterpillar-induced pain and forms the foundation for elucidating the pain-producing mechanism.
| Original language | English (US) |
|---|---|
| Journal | Toxins |
| Volume | 11 |
| Issue number | 12 |
| DOIs | |
| State | Published - Nov 27 2019 |
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Keywords
- caterpillar
- Latoia consocia
- pain
- trpv1
- venom
ASJC Scopus subject areas
- Toxicology
- Health, Toxicology and Mutagenesis
Cite this
The Latoia consocia Caterpillar Induces Pain by Targeting Nociceptive Ion Channel TRPV1. / Yao, Zhihao; Kamau, Peter Muiruri; Han, Yalan; Hu, Jingmei; Luo, Anna; Luo, Lei; Zheng, Jie; Tian, Yuhua; Lai, Ren.
In: Toxins, Vol. 11, No. 12, 27.11.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The Latoia consocia Caterpillar Induces Pain by Targeting Nociceptive Ion Channel TRPV1
AU - Yao, Zhihao
AU - Kamau, Peter Muiruri
AU - Han, Yalan
AU - Hu, Jingmei
AU - Luo, Anna
AU - Luo, Lei
AU - Zheng, Jie
AU - Tian, Yuhua
AU - Lai, Ren
PY - 2019/11/27
Y1 - 2019/11/27
N2 - Accidental contact with caterpillar bristles causes local symptoms such as severe pain, intense heat, edema, erythema, and pruritus. However, there is little functional evidence to indicate a potential mechanism. In this study, we analyzed the biological characteristics of the crude venom from the larval stage of Latoia consocia living in South-West China. Intraplantar injection of the venom into the hind paws of mice induced severe acute pain behaviors in wild type (WT) mice; the responses were much reduced in TRPV1-deficit (TRPV1 KO) mice. The TRPV1-specific inhibitor, capsazepine, significantly attenuated the pain behaviors. Furthermore, the crude venom evoked strong calcium signals in the dorsal root ganglion (DRG) neurons of WT mice but not those of TRPV1 KO mice. Among the pain-related ion channels we tested, the crude venom only activated the TRPV1 channel. To better understand the venom components, we analyzed the transcriptome of the L. consocia sebaceous gland region. Our study suggests that TRPV1 serves as a primary nociceptor in caterpillar-induced pain and forms the foundation for elucidating the pain-producing mechanism.
AB - Accidental contact with caterpillar bristles causes local symptoms such as severe pain, intense heat, edema, erythema, and pruritus. However, there is little functional evidence to indicate a potential mechanism. In this study, we analyzed the biological characteristics of the crude venom from the larval stage of Latoia consocia living in South-West China. Intraplantar injection of the venom into the hind paws of mice induced severe acute pain behaviors in wild type (WT) mice; the responses were much reduced in TRPV1-deficit (TRPV1 KO) mice. The TRPV1-specific inhibitor, capsazepine, significantly attenuated the pain behaviors. Furthermore, the crude venom evoked strong calcium signals in the dorsal root ganglion (DRG) neurons of WT mice but not those of TRPV1 KO mice. Among the pain-related ion channels we tested, the crude venom only activated the TRPV1 channel. To better understand the venom components, we analyzed the transcriptome of the L. consocia sebaceous gland region. Our study suggests that TRPV1 serves as a primary nociceptor in caterpillar-induced pain and forms the foundation for elucidating the pain-producing mechanism.
KW - caterpillar
KW - Latoia consocia
KW - pain
KW - trpv1
KW - venom
UR - http://www.scopus.com/inward/record.url?scp=85075776363&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85075776363&partnerID=8YFLogxK
U2 - 10.3390/toxins11120695
DO - 10.3390/toxins11120695
M3 - Article
C2 - 31783580
AN - SCOPUS:85075776363
VL - 11
JO - Toxins
JF - Toxins
SN - 2072-6651
IS - 12
ER -