The L4F3 antigen is expressed by unipotent and multipotent colony-forming cells but not by their precursors

R. G. Andrews, M. Takahashi, G. M. Segal, Jerry S Powell, I. D. Bernstein, J. W. Singer

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83 Scopus citations

Abstract

Antibody L4F3 is a murine monoclonal antibody that recognizes an antigen expressed on in vitro colony-forming cells, including virtually all CFU-GM, CFU-Meg, BFU-E, and CFU-Mix. In the present study we examined whether cells that do not express the L4F3 antigen include precursors of hematopoietic colony-forming cells. Colony-forming cells were depleted from marrow by treatment with L4F3 and complement. The remaining cells generated CFU-GM, BFU-E, and CFU-Mix when cultured in the presence of irradiated adherent cell layers from long-term marrow cultures. Marrow cells not expressing the L4F3 antigen, which were separated by cell-sorting techniques, were depleted of colony-forming cells but nevertheless generated CFU-GM when cultured over irradiated adherent cell layers. These data suggest that there are marrow precursors that do not express the L4F3 antigen and that give rise to colony-forming cells of multiple types. Negative selection techniques should allow the enrichment of these precursors of colony-forming cells, thereby enabling direct studies of these immature stem cells.

Original languageEnglish (US)
Pages (from-to)1030-1035
Number of pages6
JournalBlood
Volume68
Issue number5
StatePublished - 1986
Externally publishedYes

ASJC Scopus subject areas

  • Hematology

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    Andrews, R. G., Takahashi, M., Segal, G. M., Powell, J. S., Bernstein, I. D., & Singer, J. W. (1986). The L4F3 antigen is expressed by unipotent and multipotent colony-forming cells but not by their precursors. Blood, 68(5), 1030-1035.