The L4F3 antigen is expressed by unipotent and multipotent colony-forming cells but not by their precursors

R. G. Andrews, M. Takahashi, G. M. Segal, Jerry S Powell, I. D. Bernstein, J. W. Singer

Research output: Contribution to journalArticle

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Abstract

Antibody L4F3 is a murine monoclonal antibody that recognizes an antigen expressed on in vitro colony-forming cells, including virtually all CFU-GM, CFU-Meg, BFU-E, and CFU-Mix. In the present study we examined whether cells that do not express the L4F3 antigen include precursors of hematopoietic colony-forming cells. Colony-forming cells were depleted from marrow by treatment with L4F3 and complement. The remaining cells generated CFU-GM, BFU-E, and CFU-Mix when cultured in the presence of irradiated adherent cell layers from long-term marrow cultures. Marrow cells not expressing the L4F3 antigen, which were separated by cell-sorting techniques, were depleted of colony-forming cells but nevertheless generated CFU-GM when cultured over irradiated adherent cell layers. These data suggest that there are marrow precursors that do not express the L4F3 antigen and that give rise to colony-forming cells of multiple types. Negative selection techniques should allow the enrichment of these precursors of colony-forming cells, thereby enabling direct studies of these immature stem cells.

Original languageEnglish (US)
Pages (from-to)1030-1035
Number of pages6
JournalBlood
Volume68
Issue number5
StatePublished - 1986
Externally publishedYes

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Antigens
Cells
Granulocyte-Macrophage Progenitor Cells
Stem cells
Bone Marrow
Sorting
Cell culture
Erythroid Precursor Cells
Monoclonal Antibodies
Antibodies
Stem Cells

ASJC Scopus subject areas

  • Hematology

Cite this

Andrews, R. G., Takahashi, M., Segal, G. M., Powell, J. S., Bernstein, I. D., & Singer, J. W. (1986). The L4F3 antigen is expressed by unipotent and multipotent colony-forming cells but not by their precursors. Blood, 68(5), 1030-1035.

The L4F3 antigen is expressed by unipotent and multipotent colony-forming cells but not by their precursors. / Andrews, R. G.; Takahashi, M.; Segal, G. M.; Powell, Jerry S; Bernstein, I. D.; Singer, J. W.

In: Blood, Vol. 68, No. 5, 1986, p. 1030-1035.

Research output: Contribution to journalArticle

Andrews, RG, Takahashi, M, Segal, GM, Powell, JS, Bernstein, ID & Singer, JW 1986, 'The L4F3 antigen is expressed by unipotent and multipotent colony-forming cells but not by their precursors', Blood, vol. 68, no. 5, pp. 1030-1035.
Andrews RG, Takahashi M, Segal GM, Powell JS, Bernstein ID, Singer JW. The L4F3 antigen is expressed by unipotent and multipotent colony-forming cells but not by their precursors. Blood. 1986;68(5):1030-1035.
Andrews, R. G. ; Takahashi, M. ; Segal, G. M. ; Powell, Jerry S ; Bernstein, I. D. ; Singer, J. W. / The L4F3 antigen is expressed by unipotent and multipotent colony-forming cells but not by their precursors. In: Blood. 1986 ; Vol. 68, No. 5. pp. 1030-1035.
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