The kinesin-like protein KLP61F is essential for mitosis in Drosophila

M. M S Heck, A. Pereira, Patricia Pesavento, Y. Yannoni, A. C. Spradling, L. S B Goldstein

Research output: Contribution to journalArticle

236 Scopus citations

Abstract

We report here that disruption of a recently discovered kinesin-like protein in Drosophila melanogaster, KLP61F, results in a mitotic mutation lethal to the organism. We show that in the absence of KLP61F function, spindle poles fail to separate, resulting in the formation of monopolar mitotic spindles. The resulting phenotype of metaphase arrest with polyploid cells is reminiscent of that seen in the fungal bimC and cut7 mutations, where it has also been shown that spindle pole bodies are not segregated. KLP61F is specifically expressed in proliferating tissues during embryonic and larval development, consistent with a primary role in cell division. The structural and functional homology of the KLP61F, bimC, cut7, and Eg5 kinesin-like proteins demonstrates the existence of a conserved family of kinesin-like molecules important for spindle pole separation and mitotic spindle dynamics.

Original languageEnglish (US)
Pages (from-to)665-679
Number of pages15
JournalJournal of Cell Biology
Volume123
Issue number3
DOIs
StatePublished - 1993
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Cell Biology

Cite this

Heck, M. M. S., Pereira, A., Pesavento, P., Yannoni, Y., Spradling, A. C., & Goldstein, L. S. B. (1993). The kinesin-like protein KLP61F is essential for mitosis in Drosophila. Journal of Cell Biology, 123(3), 665-679. https://doi.org/10.1083/jcb.123.3.665