Abstract
Background Schwann cell-like cells differentiated from adipose-derived stem cells may have an important role in peripheral nerve regeneration. Herein, we document the individual effects of growth factors in Schwann cell-like differentiation medium. Methods There were 6 groups in the study. In the control group, we supplemented the rat adipose-derived stem cells with normal cell culture medium. In group 1, we fed the cells with Schwann cell-like differentiation medium (normal cell culture medium supplemented with platelet-derived growth factor, basic fibroblast growth factor, forskolin, and glial growth factor). In the other groups, we removed the components of the medium one at a time from the differentiation medium so that group 2 lacked glial growth factor, group 3 lacked forskolin, group 4 lacked basic fibroblast growth factor, and group 5 lacked platelet-derived growth factor. We examined the expression of the Schwann cell-specific genes with quantitative reverse transcription polymerase chain reaction and immunofluorescence staining in each group. Results Groups 3 and 4, lacking forskolin and basic fibroblast growth factor, respectively, had the highest expression levels of integrin-β4, and p75. Group 1 showed a 3.2-fold increase in the expression of S100, but the expressions of integrin-β4 and p75 were significantly lower compared to groups 3 and 4. Group 2 [glial growth factor (-)] did not express significant levels of Schwann cell-specific genes. The gene expression profile in group 4 most closely resembled Schwann cells. Immunofluorescence staining results were parallel with the quantitative real-time polymerase chain reaction results. Conclusions Glial growth factor is a key component of Schwann cell-like differentiation medium.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 584-588 |
| Number of pages | 5 |
| Journal | Annals of Plastic Surgery |
| Volume | 74 |
| Issue number | 5 |
| DOIs | |
| State | Published - May 4 2015 |
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Keywords
- adipose-derived stem cells
- peripheral nerve injury
- Schwann cells
ASJC Scopus subject areas
- Surgery
Cite this
The Key Components of Schwann Cell-like Differentiation Medium and their Effects on Gene Expression Pattern of Adipose-Derived Stem Cells. / Orbay, Hakan; Little, Christopher J.; Lankford, Lee; Olson, Christine A.; Sahar, David E.
In: Annals of Plastic Surgery, Vol. 74, No. 5, 04.05.2015, p. 584-588.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The Key Components of Schwann Cell-like Differentiation Medium and their Effects on Gene Expression Pattern of Adipose-Derived Stem Cells
AU - Orbay, Hakan
AU - Little, Christopher J.
AU - Lankford, Lee
AU - Olson, Christine A.
AU - Sahar, David E
PY - 2015/5/4
Y1 - 2015/5/4
N2 - Background Schwann cell-like cells differentiated from adipose-derived stem cells may have an important role in peripheral nerve regeneration. Herein, we document the individual effects of growth factors in Schwann cell-like differentiation medium. Methods There were 6 groups in the study. In the control group, we supplemented the rat adipose-derived stem cells with normal cell culture medium. In group 1, we fed the cells with Schwann cell-like differentiation medium (normal cell culture medium supplemented with platelet-derived growth factor, basic fibroblast growth factor, forskolin, and glial growth factor). In the other groups, we removed the components of the medium one at a time from the differentiation medium so that group 2 lacked glial growth factor, group 3 lacked forskolin, group 4 lacked basic fibroblast growth factor, and group 5 lacked platelet-derived growth factor. We examined the expression of the Schwann cell-specific genes with quantitative reverse transcription polymerase chain reaction and immunofluorescence staining in each group. Results Groups 3 and 4, lacking forskolin and basic fibroblast growth factor, respectively, had the highest expression levels of integrin-β4, and p75. Group 1 showed a 3.2-fold increase in the expression of S100, but the expressions of integrin-β4 and p75 were significantly lower compared to groups 3 and 4. Group 2 [glial growth factor (-)] did not express significant levels of Schwann cell-specific genes. The gene expression profile in group 4 most closely resembled Schwann cells. Immunofluorescence staining results were parallel with the quantitative real-time polymerase chain reaction results. Conclusions Glial growth factor is a key component of Schwann cell-like differentiation medium.
AB - Background Schwann cell-like cells differentiated from adipose-derived stem cells may have an important role in peripheral nerve regeneration. Herein, we document the individual effects of growth factors in Schwann cell-like differentiation medium. Methods There were 6 groups in the study. In the control group, we supplemented the rat adipose-derived stem cells with normal cell culture medium. In group 1, we fed the cells with Schwann cell-like differentiation medium (normal cell culture medium supplemented with platelet-derived growth factor, basic fibroblast growth factor, forskolin, and glial growth factor). In the other groups, we removed the components of the medium one at a time from the differentiation medium so that group 2 lacked glial growth factor, group 3 lacked forskolin, group 4 lacked basic fibroblast growth factor, and group 5 lacked platelet-derived growth factor. We examined the expression of the Schwann cell-specific genes with quantitative reverse transcription polymerase chain reaction and immunofluorescence staining in each group. Results Groups 3 and 4, lacking forskolin and basic fibroblast growth factor, respectively, had the highest expression levels of integrin-β4, and p75. Group 1 showed a 3.2-fold increase in the expression of S100, but the expressions of integrin-β4 and p75 were significantly lower compared to groups 3 and 4. Group 2 [glial growth factor (-)] did not express significant levels of Schwann cell-specific genes. The gene expression profile in group 4 most closely resembled Schwann cells. Immunofluorescence staining results were parallel with the quantitative real-time polymerase chain reaction results. Conclusions Glial growth factor is a key component of Schwann cell-like differentiation medium.
KW - adipose-derived stem cells
KW - peripheral nerve injury
KW - Schwann cells
UR - http://www.scopus.com/inward/record.url?scp=84942825333&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84942825333&partnerID=8YFLogxK
U2 - 10.1097/SAP.0000000000000436
DO - 10.1097/SAP.0000000000000436
M3 - Article
VL - 74
SP - 584
EP - 588
JO - Annals of Plastic Surgery
JF - Annals of Plastic Surgery
SN - 0148-7043
IS - 5
ER -