The Key Components of Schwann Cell-like Differentiation Medium and their Effects on Gene Expression Pattern of Adipose-Derived Stem Cells

Hakan Orbay, Christopher J. Little, Lee Lankford, Christine A. Olson, David E Sahar

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background Schwann cell-like cells differentiated from adipose-derived stem cells may have an important role in peripheral nerve regeneration. Herein, we document the individual effects of growth factors in Schwann cell-like differentiation medium. Methods There were 6 groups in the study. In the control group, we supplemented the rat adipose-derived stem cells with normal cell culture medium. In group 1, we fed the cells with Schwann cell-like differentiation medium (normal cell culture medium supplemented with platelet-derived growth factor, basic fibroblast growth factor, forskolin, and glial growth factor). In the other groups, we removed the components of the medium one at a time from the differentiation medium so that group 2 lacked glial growth factor, group 3 lacked forskolin, group 4 lacked basic fibroblast growth factor, and group 5 lacked platelet-derived growth factor. We examined the expression of the Schwann cell-specific genes with quantitative reverse transcription polymerase chain reaction and immunofluorescence staining in each group. Results Groups 3 and 4, lacking forskolin and basic fibroblast growth factor, respectively, had the highest expression levels of integrin-β4, and p75. Group 1 showed a 3.2-fold increase in the expression of S100, but the expressions of integrin-β4 and p75 were significantly lower compared to groups 3 and 4. Group 2 [glial growth factor (-)] did not express significant levels of Schwann cell-specific genes. The gene expression profile in group 4 most closely resembled Schwann cells. Immunofluorescence staining results were parallel with the quantitative real-time polymerase chain reaction results. Conclusions Glial growth factor is a key component of Schwann cell-like differentiation medium.

Original languageEnglish (US)
Pages (from-to)584-588
Number of pages5
JournalAnnals of Plastic Surgery
Volume74
Issue number5
DOIs
StatePublished - May 4 2015

Fingerprint

Schwann Cells
Cell Differentiation
Neuregulin-1
Stem Cells
Gene Expression
Fibroblast Growth Factor 2
Colforsin
Platelet-Derived Growth Factor
Integrins
Fluorescent Antibody Technique
Culture Media
Fibroblast Growth Factor 5
Cell Culture Techniques
Staining and Labeling
Nerve Regeneration
Peripheral Nerves
Transcriptome
Genes
Reverse Transcription
Real-Time Polymerase Chain Reaction

Keywords

  • adipose-derived stem cells
  • peripheral nerve injury
  • Schwann cells

ASJC Scopus subject areas

  • Surgery

Cite this

The Key Components of Schwann Cell-like Differentiation Medium and their Effects on Gene Expression Pattern of Adipose-Derived Stem Cells. / Orbay, Hakan; Little, Christopher J.; Lankford, Lee; Olson, Christine A.; Sahar, David E.

In: Annals of Plastic Surgery, Vol. 74, No. 5, 04.05.2015, p. 584-588.

Research output: Contribution to journalArticle

Orbay, Hakan ; Little, Christopher J. ; Lankford, Lee ; Olson, Christine A. ; Sahar, David E. / The Key Components of Schwann Cell-like Differentiation Medium and their Effects on Gene Expression Pattern of Adipose-Derived Stem Cells. In: Annals of Plastic Surgery. 2015 ; Vol. 74, No. 5. pp. 584-588.
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N2 - Background Schwann cell-like cells differentiated from adipose-derived stem cells may have an important role in peripheral nerve regeneration. Herein, we document the individual effects of growth factors in Schwann cell-like differentiation medium. Methods There were 6 groups in the study. In the control group, we supplemented the rat adipose-derived stem cells with normal cell culture medium. In group 1, we fed the cells with Schwann cell-like differentiation medium (normal cell culture medium supplemented with platelet-derived growth factor, basic fibroblast growth factor, forskolin, and glial growth factor). In the other groups, we removed the components of the medium one at a time from the differentiation medium so that group 2 lacked glial growth factor, group 3 lacked forskolin, group 4 lacked basic fibroblast growth factor, and group 5 lacked platelet-derived growth factor. We examined the expression of the Schwann cell-specific genes with quantitative reverse transcription polymerase chain reaction and immunofluorescence staining in each group. Results Groups 3 and 4, lacking forskolin and basic fibroblast growth factor, respectively, had the highest expression levels of integrin-β4, and p75. Group 1 showed a 3.2-fold increase in the expression of S100, but the expressions of integrin-β4 and p75 were significantly lower compared to groups 3 and 4. Group 2 [glial growth factor (-)] did not express significant levels of Schwann cell-specific genes. The gene expression profile in group 4 most closely resembled Schwann cells. Immunofluorescence staining results were parallel with the quantitative real-time polymerase chain reaction results. Conclusions Glial growth factor is a key component of Schwann cell-like differentiation medium.

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